Remote Sensing for Precision Nitrogen Management

This book focuses on the fundamental and applied research of the non-destructive estimation and diagnosis of crop leaf and plant nitrogen status and in-season nitrogen management strategies based on leaf sensors, proximal canopy sensors, unmanned aerial vehicle remote sensing, manned aerial remote sensing and satellite remote sensing technologies. Statistical and machine learning methods are used to predict plant-nitrogen-related parameters with sensor data or sensor data together with soil, landscape, weather and/or management information. Different sensing technologies or different modelling approaches are compared and evaluated. Strategies are developed to use crop sensing data for in-season nitrogen recommendations to improve nitrogen use efficiency and protect the environment

Leaf nitrogen determination using non-destructive techniques–A review

© 2017 Taylor & Francis Group, LLC. The optimisation of plant nitrogen-use-efficiency (NUE) has a direct impact on increasing crop production by optimising use of nitrogen fertiliser. Moreover, it protects environment from negative effects of nitrate leaching and nitrous oxide production. Accordingly, nitrogen (N) management in agriculture systems has been major focus of many researchers. Improvement of NUE can be achieved through several methods including more accurate measurement of foliar N contents of crops during different growth phases. There are two types of methods to diagnose foliar N status: destructive and non-destructive. Destructive methods are expensive and time-consuming, as they require tissue sampling and subsequent laboratory analysis. Thus, many farmers find destructive methods to be less attractive. Non-destructive methods are rapid and less expensive but are usually less accurate. Accordingly, improving the accuracy of non-destructive N estimations has become a common goal of many researchers, and various methods varying in complexity and optimality have been proposed for this purpose. This paper reviews various commonly used non-destructive methods for estimating foliar N status of plants

High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms

Crop yields need to be improved in a sustainable manner to meet the expected worldwide increase in population over the coming decades as well as the effects of anticipated climate change. Recently, genomics-assisted breeding has become a popular approach to food security; in this regard, the crop breeding community must better link the relationships between the phenotype and the genotype. While high-throughput genotyping is feasible at a low cost, highthroughput crop phenotyping methods and data analytical capacities need to be improved. High-throughput phenotyping offers a powerful way to assess particular phenotypes in large-scale experiments, using high-tech sensors, advanced robotics, and imageprocessing systems to monitor and quantify plants in breeding nurseries and field experiments at multiple scales. In addition, new bioinformatics platforms are able to embrace large-scale, multidimensional phenotypic datasets. Through the combined analysis of phenotyping and genotyping data, environmental responses and gene functions can now be dissected at unprecedented resolution. This will aid in finding solutions to currently limited and incremental improvements in crop yields

Monitoring Agronomic Parameters of Winter Wheat Crops with Low-Cost UAV Imagery

Monitoring the dynamics in wheat crops requires near-term observations with high spatial resolution due to the complex factors influencing wheat growth variability. We studied the prospects for monitoring the biophysical parameters and nitrogen status in wheat crops with low-cost imagery acquired from unmanned aerial vehicles (UAV) over an 11 ha field. Flight missions were conducted at approximately 50 m in altitude with a commercial copter and camera system—three missions were performed between booting and maturing of the wheat plants and one mission after tillage. Ultra-high resolution orthoimages of 1.2 cm·px−1 and surface models were generated for each mission from the standard red, green and blue (RGB) aerial images. The image variables were extracted from image tone and surface models, e.g., RGB ratios, crop coverage and plant height. During each mission, 20 plots within the wheat canopy with 1 × 1 m2 sample support were selected in the field, and the leaf area index, plant height, fresh and dry biomass and nitrogen concentrations were measured. From the generated UAV imagery, we were able to follow the changes in early senescence at the individual plant level in the wheat crops. Changes in the pattern of the wheat canopy varied drastically from one mission to the next, which supported the need for instantaneous observations, as delivered by UAV imagery. The correlations between the biophysical parameters and image variables were highly significant during each mission, and the regression models calculated with the principal components of the image variables yielded R2 values between 0.70 and 0.97. In contrast, the models of the nitrogen concentrations yielded low R2 values with the best model obtained at flowering (R2 = 0.65). The nitrogen nutrition index was calculated with an accuracy of 0.10 to 0.11 NNI for each mission. For all models, information about the surface models and image tone was important. We conclude that low-cost RGB UAV imagery will strongly aid farmers in observing biophysical characteristics, but it is limited for observing the nitrogen status within wheat crops

Use of an Active Canopy Sensor Mounted on an Unmanned Aerial Vehicle to Monitor the Growth and Nitrogen Status of Winter Wheat

Using remote sensing to rapidly acquire large-area crop growth information (e.g., shoot biomass, nitrogen status) is an urgent demand for modern crop production; unmanned aerial vehicle (UAV) acts as an effective monitoring platform. In order to improve the practicability and efficiency of UAV based monitoring technique, four field experiments involving different nitrogen (N) rates (0–360 kg N ha−1 ) and seven winter wheat (Triticum aestivum L.) varieties were conducted at different eco-sites (Sihong, Rugao, and Xinghua) during 2015–2019. A multispectral active canopy sensor (RapidSCAN CS-45; Holland Scientific Inc., Lincoln, NE, USA) mounted on a multirotor UAV platform was used to collect the canopy spectral reflectance data of winter wheat at key growth stages, three growth parameters (leaf area index (LAI), leaf dry matter (LDM), plant dry matter (PDM)) and three N indicators (leaf N accumulation (LNA), plant N accumulation (PNA) and N nutrition index (NNI)) were measured synchronously. The quantitative linear relationships between spectral data and six growth indices were systematically analyzed. For monitoring growth and N nutrition status at Feekes stages 6.0–10.0, 10.3–11.1 or entire growth stages, red edge ratio vegetation index (RERVI), red edge chlorophyll index (CIRE) and difference vegetation index (DVI) performed the best among the red edge band-based and red-based vegetation indices, respectively. Across all growth stages, DVI was highly correlated with LAI (R2 = 0.78), LDM (R2 = 0.61), PDM (R2 = 0.63), LNA (R2 = 0.65) and PNA (R2 = 0.73), whereas the relationships between RERVI (R2 = 0.62), CIRE (R2 = 0.62) and NNI had high coefficients of determination. The developed models performed better in monitoring growth indices and N status at Feekes stages 10.3–11.1 than Feekes stages 6.0–10.0. To sum it up, the UAV-mounted active sensor system is able to rapidly monitor the growth and N nutrition status of winter wheat and can be deployed for UAV-based remote-sensing of crops

Earth Observations and Integrative Models in Support of Food and Water Security

Global food production depends upon many factors that Earth observing satellites routinely measure about water, energy, weather, and ecosystems. Increasingly sophisticated, publicly-available satellite data products can improve efficiencies in resource management and provide earlier indication of environmental disruption. Satellite remote sensing provides a consistent, long-term record that can be used effectively to detect large-scale features over time, such as a developing drought. Accuracy and capabilities have increased along with the range of Earth observations and derived products that can support food security decisions with actionable information. This paper highlights major capabilities facilitated by satellite observations and physical models that have been developed and validated using remotely-sensed observations. Although we primarily focus on variables relevant to agriculture, we also include a brief description of the growing use of Earth observations in support of aquaculture and fisheries

Digital phenotyping and genotype-to-phenotype (G2P) models to predict complex traits in cereal crops

The revolution in digital phenotyping combined with the new layers of omics and envirotyping tools offers great promise to improve selection and accelerate genetic gains for crop improvement. This chapter examines the latest methods involving digital phenotyping tools to predict complex traits in cereals crops. The chapter has two parts. In the first part, entitled “Digital phenotyping as a tool to support breeding programs”, the secondary phenotypes measured by high-throughput plant phenotyping that are potentially useful for breeding are reviewed. In the second part, “Implementing complex G2P models in breeding programs”, the integration of data from digital phenotyping into genotype to phenotype (G2P) models to improve the prediction of complex traits using genomic information is discussed. The current status of statistical models to incorporate secondary traits in univariate and multivariate models, as well as how to better handle longitudinal (for example light interception, biomass accumulation, canopy height) traits, is reviewe

Influence of nitrogen and water supply on evapotranspiration, yield and agronomic water use efficiency of winter wheat

The predicted climate change for North West Europe, which will be characterized by higher temperatures and lower rainfall during the summer months, will challenge future food production. Against this background, the question is how nitrogen supply influences the evapotranspiration (ETa) and the agronomic water-use-efficiency (WUEy). Literature regarding drought stress reactions of winter wheat (Triticum aestivum, L.) grown in the temperate humid climate of North West Europe is scarce. Because of this a field trial with variable nitrogen and water supply was conducted during 2013-2015 at the Research Center for Crop Nutrition Hanninghof in Dülmen. To induce different water regimes (fully watered control, early drought, early and late drought) rain-out-shelter and drip irrigation systems were installed. Different N-supply treatments (unfertilized control, 120 and 230 kg N ha-1) were induced using calcium ammonium nitrate (27% N). A realistic calculation of ETa is the key for the quantification of the impact of nitrogen on WUEy. Therefore two different calculation approaches for estimating ETa according to the FAO 56 method were compared. ETa was calculated based on handspectrometer measurements converted to crop coefficients (Kc-values) (NDVI-approach) and on the other hand based on published Kc-values and Kc duration periods (tabulated-approach) for plots that were not water and nitrogen limited. In general, it could be concluded that the method for estimating ETa according to FAO 56 showed realistic results. Furthermore, the data showed that the NDVI-approach, in contrast to the tabulated-approach, allowed a realistic calculation of ETa. Tabulated values could only be used retrospectively for estimating ETa, whereas the NDVI-approach can take growth influencing parameters (weather, pests, lack of nutrients) into account and can therefore be used to quantify ETa during the vegetation season. For this reason we used the NDVI-approach to calculate the ETa for plots that were limited in their water and nitrogen supply. If water was not limited a higher nitrogen rate increased grain yields of wheat more than ETa. The positive effect of nitrogen fertilization on WUEy was mainly caused by a reduction of soil evaporation. This effect was more pronounced under wet than under drought conditions. The results of this study also showed that the NDVI-approach can be used not only to quantify ETa but also to measure yield reductions caused by drought stress (Ky-value) during the vegetation period. Drought stress during booting also caused grain yield reductions. This study showed that a high N-supply compared to moderate N-supply under early drought and continuous drought conditions increased both grain yields and ETa in a more water use efficient way. We refer this positive N-effect to the fully water saturated soils at the start of the vegetation period in spring.Die Prognose der klimatischen Veränderungen in Nordwesteuropa, welche im Vergleich zur derzeitigen Situation erhöhte Temperaturen und geringere Niederschläge in den Sommer-monaten vorhersagt, wird die zukünftige Nahrungsmittelproduktion vor neue Heraus-forderungen stellen. Vor diesem Hintergrund stellt sich die Frage, welchen Einfluss die Stickstoffdüngung auf die Evapotranspiration (ETa) und die agronomische Effizienz der Wassernutzung (WUEY) hat. Da aus der Literatur wenig über Trockenstressreaktionen von Winterweizen (Triticum aestivum, L.) in temperat humiden Klimaten in Nord-West Europa bekannt ist, wurde in den Jahren 2013-2015 Feldversuche mit unterschiedlichem Stickstoff- und Wasserangebot am Institut für Pflanzenernährung und Umweltforschung in Dülmen durchgeführt. Zur Einstellung der unterschiedlichen Wasserversorgung (voll gewässerte Kontrolle, früher Trockenstress, früher und später Trockenstress) wurden Regenabdeckungen und Tropfbewässerungssysteme eingesetzt. Eine unterschiedliche N-Versorgung (ungedüngte Kontrolle, 120 und 230 kg N ha-1) wurde mittels Düngung mit Kalkammonsalpeter (27% N) eingestellt. Eine möglichst realistische Berechnung der ETa ist für die Quantifizierung des Einflusses der Stickstoffversorgung auf die WUEy von zentraler Bedeutung. Zunächst wurden deshalb zwei Berechnungsmethoden für die Abschätzung der ETa nach Methode FAO 56 verglichen. Die ETa wurde anhand von handspektrometrisch gemessenen Pflanzen-koeffizienten (Kc-Werten) (NDVI-Ansatz) und auf Grundlage publizierter Kc-Werte und Kc-Phasenlängen (tabellierter-Ansatz) für nicht Wasser- und Stickstoff-limitierte Weizenbestände berechnet. Insgesamt zeigte sich, dass die ETa-Berechnungsmethode nach FAO 56 die Wasserverbräuche der Pflanzenbestände sehr realistisch abbildete. Die Daten zeigen weiterhin, dass der NDVI-Ansatz im Vergleich mit dem tabellierten-Ansatz eine realistische Berechnung der ETa ermöglicht. Tabellierte Werte können lediglich retrospektiv für eine Berechnung der ETa herangezogen werden, währenddessen der NDVI-Ansatz auch während der Vegetationsperiode zur Abschätzung der ETa genutzt werden kann und wachstums-beeinflussende Faktoren (Wetter, Nährstoffmangel, Krankheiten) berücksichtigt. Deshalb erfolgte die Berechnung der ETa für Stickstoff- und Wasser-limitierte Prüfglieder mit dem NDVI-Ansatz. Bei ausreichendem Wasserangebot erhöhte die Stickstoffdüngung den Korn-ertrag vergleichsweise stärker als die ETa. Der positive Effekt der Stickstoffdüngung auf die WUEy beruhte hauptsächlich auf einer relativen Verminderung der Bodenevaporation. Dieser Effekt war unter Bedingungen ausreichender Wasserversorgung ausgeprägter als unter Wasser-limitierten Bedingungen. Die Ergebnisse zeigen, dass mittels des NDVI-Ansatzes nicht nur die ETa, sondern auch das Ausmaß der Trockenstress bedingten Ertragsreduktion (Ky-Wert) quantifiziert werden konnten. Weiterhin stellte sich heraus, dass auch ein Trockenstress während des Schossens den Ertrag negativ beeinflusste. Die Ergebnisse dieser Arbeit zeigten, dass eine hohe N-Versorgung im Vergleich zu einer moderaten N-Düngung unter frühem und andauernden Trockenstress zu höheren Kornerträgen führte und gleichzeitig eine höhere ETa verursachte, was in einer höheren WUEy resultierte. Wir schreiben den positiven N-Effekt den nahezu wassergesättigten Böden zu Vegetationsbeginn nach Winter zu

Combining remote sensing and crop modeling techniques to derive a nitrogen fertilizer application strategy

The crucial question in this thesis was how can remote sensing data and crop models be used to derive a N fertilizer strategy that is capable to lower the environmental side effects of N fertilizer application. This raised the following detailed objectives: The first objective (i) how N content determination via spectral reflectance is influenced by different leaves and positions on the leaf was investigated in Publication I. Different wheat plants were cultivated under different N levels and under drought stress in two hydroponic greenhouse trials. Spectral reflectance measurements were taken from three leaves and at three positions on the leaf for each plant. In total, 16 vegetation indices broadly used in the literature were calculated based on the spectral reflectance for each combination of leaf and position. The plant N content was determined by lab analyses. Neither the position on the leaf nor leaf number had an impact on the accuracy of plant N determination via spectral reflectance measurements. Therefore measurements taken at the canopy level seem to be a valid approach. However, if other stress symptoms like drought or disease infection occur, a differentiation between leaves and positions on the leaf might play a more crucial role. Publication II dealt with the second objective on (ii), how to incorporate leaf disease into the DSSAT wheat model to enable the simulation of the impact of leaf disease on yield. An integration of sensor information in crop growth models requires the update of model state variables. A model extension was developed by adding a pest damage module to the existing wheat model. The approach was tested on a two-year dataset from Argentina with different wheat cultivars and on a one-year dataset from Germany with different inoculum levels of septoria tritici blotch (STB). After the integration of disease infection, the accuracy of the simulated yield and leaf area index (LAI) was improved. The Root mean squared error (RMSE) values for yield (1144 kg ha−1) and LAI (1.19 m2 m−2) were reduced by half (499 kg ha−1) for yield and LAI (0.69 m2 m−2). A sensitivity analysis also showed a strong responsiveness of the model by the integration of different STB disease infection scenarios. Increasing the modeling accuracy even further a MM approach seems to be suitable. Assembling more models increases the complexity of the simulation and the involved calibration procedure especially if the user is not familiar with all models. To avoid these conflicts, Publication III evaluated the third objective (iii) if an automatic calibration procedure in a MM approach for winter wheat can eliminate the subjectivity factor in model calibration. The model calibration was performed on a 4-yr N wheat fertilizer trial in southwest Germany. The evaluation mean showed satisfying results for the calibration (d-Index 0.93) and evaluation dataset (d-Index 0.81). This lead to the fourth (iv) objective to use a MM approach to improve the overall modeling accuracy. The evaluation of a fertilizer trial showed an improved modeling accuracy in most cases, especially in the drought season 2018. Based on the combination of a MM approach and the incorporation of sensor data, a Nitrogen Application Prescription System (NAPS) was developed. The initial NAPS setup requires long term recorded data (yield, weather, and soil) to ensure proper MM calibration. After calibration, the current growing season conditions are required (weather, management information) until the N application date. Afterward, the NAPS incorporates remote sensing information and generated weather for running future N application scenarios. The selection of the proper amount of N is determined by economic and ecological criteria. Furthermore, in order to account for differences in in-field variabilities and to deliver a N prescription site-specifically, the NAPS concept has to be applied on a geospatial scale by adjusting soil parameters spatially. The NAPS concept has the potential to adjust the N application more economically and ecologically by using current sensor data, historical yield records, and future weather prediction to derive a more precise N application strategy. Finally, this concept exhibits the potential for reconciliation of the issue of an economic, agricultural production without harming the environment.In dieser Arbeit wurde eruiert, ob mit Hilfe von Sensordaten und Pflanzenwachstumsmodellen eine N-Düngemittelstrategie abgeleitet werden kann, die in der Lage ist die ökologischen Belastung zu verringern. Dies umfasste die Evaluation folgender Fragestellungen: (I) Wird die spektrale Reflexion und somit die Bestimmung der N-Konzentration durch die Messung an verschiedenen Blattetagen und -Positionen beeinflusst (Publikation I)? Für die Klärung dieser ersten Frage wurden in zwei hydroponischen Gewächshausversuchen Weizenpflanzen bei unterschiedlicher N-Exposition und Trockenstress kultiviert. Für jede Pflanze wurden spektrale Reflexionsmessungen an drei Blattetagen und an drei Positionen auf dem Blatt durchgeführt. Insgesamt wurden die 16 üblichsten auf spektraler Reflexion basierenden Vegetationsindizes für jede Kombination von Blattetage und -Position berechnet. Die N-Konzentration der Pflanze wurde durch Laboranalysen bestimmt. Weder die Position auf dem Blatt noch die Blattetage hatten einen Einfluss auf die Genauigkeit der Bestimmung der N-Konzentration der Pflanze durch spektrale Reflexionsmessungen. Daher sind Messungen auf Bestandsebene ausreichend. Falls jedoch weitere Stressfaktoren wie Trockenheit oder Krankheitsbefall auftreten, kann eine Differenzierung zwischen verschiedenen Blattetagen notwendig oder von Vorteil sein. In der nächsten Fragestellung (Publikation II) wurde untersucht, wie Blattkrankheiten in ein DSSAT-Weizenmodell integriert werden können, um so die Auswirkungen von Blattkrankheiten auf den Ertrag zu simulieren. Eine Modellerweiterung wurde entwickelt, durch die Integration eines Blattkrankheitsmoduls in das bestehende DSSAT Weizenmodell. Das Modul simuliert die Auswirkungen des täglichen Schadens durch die Krankheit auf die Photosynthese und den Blattflächenindex. Der Ansatz wurde an einem zweijährigen Datensatz aus Argentinien mit verschiedenen Weizensorten und an einem einjährigen Datensatz aus Deutschland mit verschiedenen Inokulumniveaus von Septoria tritici-Blotch (STB) getestet. Die Sensitivitätsanalyse zeigte die Möglichkeit des Modells, den Ertrag in einer exponentiellen Beziehung mit zunehmendem Infektionsgrad (0-70%) zu reduzieren. Das erweiterte Modell stellt somit eine Möglichkeit dar, STB-Infektionen standortspezifisch in Verbindung mit verfügbaren Sensordaten zu simulieren. Um die Modellierungsgenauigkeit noch weiter zu erhöhen, wurde der Einsatz eines MM-Ansatz geprüft. Die Kombination von verschiedenen Modellen erhöht die Komplexität der Simulation und des damit verbundenen Kalibrierungsverfahrens, insbesondere wenn der Benutzer nicht mit allen Modellen vertraut ist. Die dritte Fragestellung (iii) untersuchte daher, ob objektive Kalibrierungsergebnisse gewährleitet werden könnten, wenn die cultivar coefficients im Modell auf Basis tatsächlich gemessener Daten mittels eines neu entwickelten automatischen Calibrator-Programms optimiert wurden. Die Modellkalibrierung wurde an einem 4-jährigen-Weizendüngungsversuch in Südwestdeutschland durchgeführt. Die statistische Auswertung des Kalibrierverfahrens zeigte zufriedenstellende Ergebnisse und führte zur vierten Fragestellung. Die vierte Fragestellung befasste sich mit dem Thema, ob ein MM-Ansatz die Gesamtmodelliergenauigkeit verbessern kann. Die Auswertung des Düngemittelversuchs zeigte in den meisten Fällen eine verbesserte Modellierungsgenauigkeit, insbesondere in einem durch Wasserstress geprägten Versuchsjahr wie 2018. Unter Verwendung eines MM-Ansatzes, durch Anpassung der Modellvariablen und durch die Integration von Sensordaten wurde ein Nitrogen Application Prescription System (NAPS) entwickelt. Eine Voraussetzung für das NAPS-Konzepts ist das Vorhandensein von Langzeit-Daten (Ertrag, Klima- und Bodenbedingungen), um eine korrekte MM-Kalibrierung zu gewährleisten. Nach der Kalibrierung werden die Bedingungen der aktuellen Wachstumssaison (Wetter, Managementinformationen) bis zum Düngetermin benötigt. Anschließend berechnet das NAPS basierend auf Sensorinformationen und simulierten Wetterbedingungen verschiedene Düngeszenarien. Ökonomische und ökologische Kriterien bestimmen die optimierte Düngemenge. Darüber hinaus muss das NAPS-Konzept auf räumlicher Ebene arbeiten, indem es die Bodenparameter berücksichtigt. So kann unter Beachtung der Feldvariabilität eine standortspezifische N-Ausbringung gewährleistet werden. In Summe zeigte sich, dass NAPS die Düngung an ökonomische und ökologische Faktoren anpasst, indem es aktuelle Sensordaten, historische Ertragsaufzeichnungen und zukünftige Wettervorhersagen zur Ermittlung einer präziseren N-Ausbringung nutzt. Das Konzept hat so das Potenzial, die nachteiligen Auswirkungen einer Überdüngung zu begrenzen, so dass eine umweltfreundlichere Agrarproduktion gewährleistet wird