Acoustical structured illumination for super-resolution ultrasound imaging.

Structured illumination microscopy is an optical method to increase the spatial resolution of wide-field fluorescence imaging beyond the diffraction limit by applying a spatially structured illumination light. Here, we extend this concept to facilitate super-resolution ultrasound imaging by manipulating the transmitted sound field to encode the high spatial frequencies into the observed image through aliasing. Post processing is applied to precisely shift the spectral components to their proper positions in k-space and effectively double the spatial resolution of the reconstructed image compared to one-way focusing. The method has broad application, including the detection of small lesions for early cancer diagnosis, improving the detection of the borders of organs and tumors, and enhancing visualization of vascular features. The method can be implemented with conventional ultrasound systems, without the need for additional components. The resulting image enhancement is demonstrated with both test objects and ex vivo rat metacarpals and phalanges

Enhancement of Underwater Video Mosaics for Post-Processing

Mosaics of seafloor created from still images or video acquired underwater have proved to be useful for construction of maps of forensic and archeological sites, species\u27 abundance estimates, habitat characterization, etc. Images taken by a camera mounted on a stable platform are registered (at first pair-wise and then globally) and assembled in a high resolution visual map of the surveyed area. While this map is usually sufficient for a human orientation and even quantitative measurements, it often contains artifacts that complicate an automatic post-processing (for example, extraction of shapes for organism counting, or segmentation for habitat characterization). The most prominent artifacts are inter-frame seams caused by inhomogeneous artificial illumination, and local feature misalignments due to parallax effects - result of an attempt to represent a 3D world on a 2D map. In this paper we propose two image processing techniques for mosaic quality enhancement - median mosaic-based illumination correction suppressing appearance of inter-frame seams, and micro warping decreasing influence of parallax effects

Full-field structured-illumination super-resolution X-ray transmission microscopy

Modern transmission X-ray microscopy techniques provide very high resolution at low and medium X-ray energies, but suffer from a limited field-of-view. If sub-micrometre resolution is desired, their field-of-view is typically limited to less than one millimetre. Although the field-of-view increases through combining multiple images from adjacent regions of the specimen, so does the required data acquisition time. Here, we present a method for fast full-field super-resolution transmission microscopy by structured illumination of the specimen. This technique is well-suited even for hard X-ray energies above 30 keV, where efficient optics are hard to obtain. Accordingly, investigation of optically thick specimen becomes possible with our method combining a wide field-of-view spanning multiple millimetres, or even centimetres, with sub-micron resolution and hard X-ray energies