New Electronic Interface Circuits for Humidity Measurement Based on the Current Processing Technique

The paper describes a new electronic conditioning circuit based on the current-processing technique for accurate and reliable humidity measurement, without post-processing requirements. Pseudobrookite nanocrystalline (Fe2TiO5) thick film was used as capacitive humidity transducer in the proposed design. The interface integrated circuit was realized in TSMC 0.18 mu m CMOS technology, but commercial devices were used for practical realization. The sensing principle of the sensor was obtained by converting the information on environment humidity into a frequency variable square-wave electric current signal. The proposed solution features high linearity, insensitivity to temperature, as well as low power consumption. The sensor has a linear function with relative humidity in the range of Relative Humidity (RH) 30-90 %, error below 1.5 %, and sensitivity 8.3 x 10(14) Hz/F evaluated over the full range of changes. A fast recovery without the need of any refreshing methods was observed with a change in RH. The total power dissipation of readout circuitry was 1 mW

Biomedical integrated circuit design for an electro-therapy device : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Electronics and Computer Engineering (Bioelectronics) at School of Engineering and Advanced Technology, Massey University, Albany Campus, New Zealand

Journal articles in Appendix A removed for copyright reasons. Chapters 3, 4 and 5 published respectively as: Abbas Al-Darkazly, Ibtisam A., & Hasan, S. M. Rezaul. (2016). A waveform generator circuit for extra low‐frequency CMOS micro‐power applications, International Journal of Circuit Theory and Applications, 44, 266-279. https://doi-org.ezproxy.massey.ac.nz/10.1002/cta.2074 Abbas Al-Darkazly, Ibtisam A., & Hasan, S. M. Rezaul. (2016). Dual-band waveform generator with ultra-wide low-frequency tuning-range, IEEE Access, 4, 3169-3181. DOI: 10.1109/ACCESS.2016.2557843 Abbas Al-Darkazly, Ibtisam A., & Hasan, S. M. Rezaul. (2017). Optimized low-power CMOS active-electrode-pair for low-frequency multi-channel biomedical stimulation, Microelectronics Journal, 66, 18-24. https://doi-org.ezproxy.massey.ac.nz/10.1016/j.mejo.2017.05.014A biomedical integrated circuit design (IC) is utilized for the development of a novel non-invasive electro-therapy device, for low frequency multi-channel biomedical stimulation to transform immune activity and induce anti-viral state. Biomedical integrated circuit design is an important branch of modern electronic engineering that uses the application of electronic engineering principles for biomedical disciplines, to develop bioelectronics devices that are implanted within the body and for non-invasive devices to improve patient’s lives. These devices use the application of an electric field to stimulate reactions to restore normal cell functions and activate the cells to treat a variety of disorders or disease conditions. Bioelectronics devices can be designed for use as alternative treatments to overcome the deficiencies of several conventional medical treatments. It could potentially assist as drug-free relief when therapeutic drugs become ineffective, costly, with serious side effects and cannot be replaced, loss of future treatment options, and hence, life threatening, as for drug resistant Human immunodeficiency virus (HIV-1) patients. Since the underlying mechanisms of the biological system and disease state is dominated by electrostatic interactions, specifically, the interaction between HIV-1 and the host cell that is predominantly by electrostatic interactions (protein charge-charge interaction) has an important role in its life cycle replication. At given pulses, the charge distribution and polarization of the electro-active protein molecules takes place, inducing conformation change which can enhance immune activity and inhibit the interaction of HIV-1 and host cells, disturbing its life cycle, leading to the mechanisms of the inactivation signal-induced virus death. These electrically induced protein transformations is used in this research as blood-cell treatment and as anti-HIV-1 electrotherapy. Advances in bioelectronics technology, which involve new CMOS IC design, and in bio-electrochemistry science, which include cellular function, electro-active biomolecules and their responses, have contributed to this project to develop the concept of a novel electro-therapy device, for biomedical treatment applications. This involves understanding of the underlying mechanisms of the biological system and disease condition from an electronic engineer’s point of view as well as the interface between the electronic signal and the biological cells, and how electronic devices and circuitry directly communicate with the electro-active body tissue and blood cells. This research project addresses the design and development of a novel energy efficient miniature biomedical device using a new CMOS technology. It can generate, deliver and control an appropriate periodical low frequency electrical pulses, through the low-resistance skin surface to a patient’s blood. The notable feature of such a smart device is its cellular specificity: the parameters of the generated electrical pulse which are designed and selected in order to stimulate only one particular type of tissue (blood) leaving the others unaffected. The device comprises a mixed-signal low power dualband waveform generator (WFG) chip along with a novel two band tuning system. It was fabricated using Global Foundries (GF) 8RF-DM 130-nm CMOS process with a supply voltage of ±1V for the analog circuit and +1V for logic circuits. The WFG core (band I) can be tuned in the range 6.44 kHz - 1003 kHz through bias current adjustment, while a lower frequency (band II) in the range 0.1 Hz to 502 kHz can be provided digitally. Two WFG approaches, that comprise relaxation oscillators with different relaxation timing networks, have been developed for comparison. Since the aim of this work is to transfer electrical signal in a specifically controlled fashion through the tissue, a novel low power active electrode-pair signal delivery system, compatible with human skin with high signal integrity, is developed. The circuit was fabricated in a 130-nm CMOS process using a low supply-voltage of +1.2V to deliver bi-phase square waveform signals from 16 selectable low-frequency channels. The individual active electrode can also be used to deliver mono-phase square/triangular waveform output signals. Accuracy, safety, low power, light-weight, miniature and low-cost characteristics are the main concerns. Being a miniature bioelectronics component with low power consumption, the proposed device is suitable both as a non-invasive and as an implantable biomedical device, in which WFG and electrodes circuitry can communicate with the electro-active biomolecule, strongly stimulating certain events in a complex biological system. A theoretical analysis, experiment design and performance are carried out in invitro environments to examine the effect of the designed signal on human blood cellular proteins. Proteins that display a heterogeneous structure have various conductivities and permittivity (determining the interaction with the electrical field) and possess dielectric properties with a large conformation change, undergoing structural rearrangements in response to cellular signals. The frequency-dependent dielectric present in proteins involves the redistribution and alignment of the proteins charged molecule and its polar molecule in response to an applied external electrical field can also induce conformation change. Interference polarization within proteins could interrupt the interaction between both sides of predominantly host cell proteins and of the HIV-1 infective envelope and its protein particles. This could disturb the signalling proteins for cell activation, and, hence, the virus cannot conjugate with the target cells and control the host cell protein activity. Since the virus is unable to reproduce out of a host cell, hence the virus cannot mutate and develop resistance easily, and use alternative binding and entry mechanisms as in the pharmacological approaches. After carefully studying the interaction of the HIV-1 virus and the host cell, with respect to signal transfer, CD4 receptor, co-receptors CCR5 and nuclear transport factor nucleoporins FGNup153 proteins of the lymphatic system, which are essential targets for HIV-1 infection and its life cycle replication represent an attractive target to investigate in this research project. The activities of the underlying mechanism of the target cell are then examined utilizing immunofluorescence microscopy technique with specific fluorescent labelled antibodies, and accurate results are obtained with relatively low cost. The results demonstrated that the low frequency electrical pulse could inhibit virus attachment and fusion. It is also could provide a permeability barrier, that prevents the import and export of large macromolecule virus particles through the nuclear pore complex. These effects could induce an antiviral state for a period of time, and stope HIV-1 virus replication, with no potential risks and harm to the host cells, compared to the common drugs. This is promising for the conception of HIV-1 treatment in vivo. Although further investigations are required in order to fully use the application of electrical stimulation in vivo for treatment, the result is provides the necessary impetus for the applications of low frequency electrical stimulation on human immune response. This might offer important antiviral therapy against the most devastating pathogens in human history. This doctoral research is not only of academic interest but also highly relevant to medical applications. It is considered potentially beneficial in the development of knowledge in advanced technology for electro-medical treatment devices, their design, structure and applications to extend life, and for future growth in the biotechnology industry, therefore beneficial for the patients, physicians and for humanity

Design of Signal Generators Using Active Elements Developed in I3T25 CMOS Technology Single IC Package for Illuminance to Frequency Conversion

This paper presents a compact and simple design of adjustable triangular and square wave functional generators employing fundamental cells fabricated on a single integrated circuit (IC) package. Two solutions have electronically tunable repeating frequency. The linear adjustability of repeating frequency was verified in the range between 17 and 264 kHz. The main benefits of the proposed generator are the follows: A simple adjustment of the repeating frequency by DC bias current, Schmitt trigger (threshold voltages) setting by DC driving voltage, and output levels in hundreds of mV when the complementary metal-oxide semiconductor (CMOS) process with limited supply voltage levels is used. These generators are suitable to provide a simple conversion of illuminance to frequency of oscillation that can be employed for illuminance measurement and sensing in the agriculture applications. Experimental measurements proved that the proposed concept is usable for sensing of illuminance in the range from 1 up to 500 lx. The change of illuminance within this range causes driving of bias current between 21 and 52 mu A that adjusts repeating frequency between 70 and 154 kHz with an error up to 10% between the expected and real cases

Integrated chaos generators

This paper surveys the different design issues, from mathematical model to silicon, involved on the design of integrated circuits for the generation of chaotic behavior.Comisión Interministerial de Ciencia y Tecnología 1FD97-1611(TIC)European Commission ESPRIT 3110

Uncalibrated operational amplifier-based sensor interface for capacitive/resistive sensor applications

In this paper, a new configuration of operational amplifier -based square-wave oscillator is proposed. The circuit performs an impedance-to-period (Z–T) conversion that, instead of a voltage integration typically performed by other solutions presented in the literature, is based on a voltage differentiation. This solution is suitable as first analogue uncalibrated front-end for capacitive and resistive (e.g. relative humidity and gas) sensors, working also, in the case of capacitive devices, for wide variation ranges (up to six capacitive variation decades). Moreover, through the setting of passive components, its sensitivity can be easily regulated. Experimental measurements, conducted on a prototype printed circuit board, with sample passive components and using the commercial capacitive humidity sensor Honeywell HCH-1000, have shown good linearity and accuracy in the estimation of capacitances, having a baseline or reaching a value ranging in a wide interval [picofarads–microfarads], as well as, with a lower accuracy, in the evaluation of more reduced variations of resistances, ranging from kiloohms to megaohms, also when compared with other solutions presented in the literature

Design of ASIC Based Electrical Impedance Tomography Microendoscopic System for Prostate Cancer Surgical Marginal Assessment

Prostate cancer is the second most common cancer in the United States. It is typically treated by surgically excising the cancerous section of the prostate. Because there is not always a visible distinction between the healthy and cancerous sections, surgery often leaves some cancerous tissue behind. This is referred to as a positive surgical margin and it requires adjuvant treatment with adverse side effects. Electrical impedance tomography (EIT) is a low-cost low-form-factor method that can be used to assess surgical marginal intraoperatively to ensure that no cancerous tissue is left behind. EIT-based surgical margin assessment works on the principle that the electrical properties of cancerous tissue are different from those of healthy tissue. These differences are small at lower frequencies but become more pronounced at frequencies of 1 MHz and higher. Unfortunately, previous EIT solutions for surgical marginal assessment have been limited to operating frequencies of less than 1 MHz. This thesis presents a custom application-specific integrated circuit (ASIC) analog front end for performing EIT with a signal-to-noise ratio of 75 dB up to an operating frequency of 10 MHz. The custom ASIC was integrated into a 16-electrode EIT system for surgical marginal assessment. The entire system was tested on a saline phantom with a 2 mm bead that represented a cancerous lesion. The EIT system produced single-frequency and multi-frequency images showing the presence of the inclusion

Robust low power CMOS methodologies for ISFETs instrumentation

I have developed a robust design methodology in a 0.18 [Mu]m commercial CMOS process to circumvent the performance issues of the integrated Ions Sensitive Field Effect Transistor (ISFET) for pH detection. In circuit design, I have developed frequency domain signal processing, which transforms pH information into a frequency modulated signal. The frequency modulated signal is subsequently digitized and encoded into a bit-stream of data. The architecture of the instrumentation system consists of a) A novel front-end averaging amplifier to interface an array of ISFETs for converting pH into a voltage signal, b) A high linear voltage controlled oscillator for converting the voltage signal into a frequency modulated signal, and c) Digital gates for digitizing and differentiating the frequency modulated signal into an output bit-stream. The output bit stream is indistinguishable to a 1st order sigma delta modulation, whose noise floor is shaped by +20dB/decade. The fabricated instrumentation system has a dimension of 1565 [Mu] m 1565 [Mu] m. The chip responds linearly to the pH in a chemical solution and produces a digital output, with up to an 8-bit accuracy. Most importantly, the fabricated chips do not need any post-CMOS processing for neutralizing any trapped-charged effect, which can modulate on-chip ISFETs’ threshold voltages into atypical values. As compared to other ISFET-related works in the literature, the instrumentation system proposed in this thesis can cope with the mismatched ISFETs on chip for analogue-to-digital conversions. The design methodology is thus very accurate and robust for chemical sensing

Design and Analysis of CCII-Based Oscillator with Amplitude Stabilization Employing Optocouplers for Linear Voltage Control of the Output Frequency

This paper shows the topology design of a simple second-order oscillator based on two three-port current conveyors, two resistors, and two grounded capacitors, as well as its modification to a voltage-controlled oscillator (VCO). In comparison with many previous works, the following useful conceptual novelties and improvements were made in this study. Both resistors presented in the topology can be employed to tune of the oscillation frequency by the simultaneous driving of two optocouplers with resistive output stage. The current gain of the current conveyor ensures the control of the oscillation condition. The proposed solution offers advantages (in comparison with many standard so-called single-resistance-controllable types) of improved dependence of the frequency of oscillation (FO) on a driving force (extended tuning of the FO), constant ratio of amplitudes of generated waveforms when the FO is tuned, low complexity (taking into account auxiliary circuitry for optocouplers), and comfortable tuning of the FO by a single control voltage. The oscillator produces waveforms with tunable frequency having a constant 45-degree phase shift between them. The relative sensitivities of the proposed solution achieve typical values for these second-order systems (0.5). Experimental verification confirmed the expected behavior in the operational band between 1 and 10 MHz tuned by a DC voltage from 1.7 to 5 V. This indicates a significant reduction of the driving force ratio (3:1 in our case) in comparison with standard tuning approaches required for a ratio of 10:1 for FO adjustment. Output amplitudes reached 100 and 150 mV in the observed tunability range with distortion ranging between 0.7 and 3.3

An electrocardiogram readout circuit based on CMOS operational floating current conveyor

Electrocardiogram (ECG) is used in diagnosing heart diseases. It is designed as integration between current-mode instrumentation amplifiers (CMIA) and low pass filter (LPF). Normal heart behavior can be identified simply by normal ECG that consists of signal while heart disorder can be recognized by having differences in the features of their corresponding ECG waveform. A novel integrated CMOS-based operational floating current conveyor (OFCC) circuit is proposed. OFCC is a five port general purpose analog building block which combines all the features of different current mode devices such as the second generation current conveyor (CCII), the current feedback operational amplifier (CFA), and the operational floating conveyor (OFC). The OFFC is modeled and simulated using UMC 130nm CMOS technology kit in Cadence with a supply voltage 1.2V. The ECG readout circuit has been designed using the proposed OFCC as a building block. The advantages of this: it is integrated, noise factor is small as the proposed OFCC has the lowest input noise voltage and the layout is simple as it is a single block that can be repeated several times

A 256-input micro-electrode array with integrated cmos amplifiers for neural signal recording

Thesis (Ph.D.)--Boston UniversityThe nervous system communicates and processes information through its basic structural units -- individual neurons (nerve cells). Neurons convey neural information via electrical and chemical signals, which makes electrophysiological recording techniques very important in the study of neurophysiology. Specifically, active microelectrode arrays (MEAs) with amplifiers integrated on the same substrate are used because they provide a very powerful neural electrical recording technique that can be directly interfaced to acute slices and cell cultures. 2D planer electrodes are typically used for recording from neural cultures in vitro, while in vivo recording in live animals invariably requires the use of 3D electrodes. I have designed an active MEA with neural amplifiers and 3D electrodes, all integrated on a single chip. The electrodes are commercially available 3D C4 (Controlled Collapse Chip Connect) flip-chip bonding solder balls that have a diameter of 100 µm and a pitch of 200 µm. An active MEA neural recording chip -- the Multiple-Input Neural Sensor (MINS) chip -- was designed and fabricated using the IBM BiCMOS 8HP 0.13 µm technology. The MINS IC has 256 input channels that are time-division multiplexed into two output pads. Each channel was designed to work at a 20 kHz frame rate with a total voltage gain of 60 dB per channel with an input-referred noise voltage of 5.3 µVrms over 10 Hz to 10 kHz. The entire MINS chip has an area of 4 x 4 mm^2 with 256 input C4s plus 20 wire-bond pads on two adjacent edges of the chip for power, control, and outputs. The fabricated MINS chips are wire-bonded to standard pin grid array (PGA), open-top PGA, and custom-designed printed circuit board (PCB) packages for electrical, in vitro, and in vivo testing, respectively. After process variation correction, the voltage gain of the 256 neural amplifiers, measured in vitro across several chips, has a mean value of 58.7 dB and a standard deviation of 0.37 dB. Measurements done with the electrical testing package demonstrate that the MINS IC has a flat frequency response from 0.05 Hz to 1.4 MHz, an input-referred noise voltage of 4.6 µVrms over 10 Hz to 10 kHz, an output voltage swing as large as 1.5 V peak-to-peak, and a total power consumption of 11.25 mW, or 43.9 µW per input channel