Development of ultrahigh resolution FTICR mass spectrometry methods for clinical proteomics

Fourier transform ion cyclotron resonance (FTICR) MS provides (a) ultrahigh mass resolving power that allows the analysis of large proteins and complex mixtures; (b) ultrahigh mass measurement accuracy and precision that allow a more reliable identification of the detected species; (c) a wide dynamic range that is favourable for the detection of low abundant components. Despite these excellent characteristics, MALDI-FTICR MS has been rarely used for peptide and protein profiling of large cohorts of samples. In this thesis, the development and application of novel MALDI-FTICR-MS methods for high-throughput analysis of human serum peptides and proteins is described. Due to the large biological variability of human serum, the analysis of a large cohort is needed to obtain reliable statistical results.UBL - phd migration 201

SPATIAL TRANSFORMATION PATTERN DUE TO COMMERCIAL ACTIVITY IN KAMPONG HOUSE

ABSTRACT Kampung houses are houses in kampung area of the city. Kampung House oftenly transformed into others use as urban dynamics. One of the transfomation is related to the commercial activities addition by the house owner. It make house with full private space become into mixused house with more public spaces or completely changed into full public commercial building. This study investigate the spatial transformation pattern of the kampung houses due to their commercial activities addition. Site observations, interviews and questionnaires were performed to study the spatial transformation. This study found that in kampung houses, the spatial transformation pattern was depend on type of commercial activities and owner perceptions, and there are several steps of the spatial transformation related the commercial activity addition. Keywords: spatial transformation pattern; commercial activity; owner perception, kampung house; adaptabilit