Arbuscular mycorrhizal fungal communities change among three stages of primary sand dune succession but do not alter plant growth

Plant interactions with soil biota could have a significant impact on plant successional trajectory by benefiting plants in a particular successional stage over others. The influence of soil mutualists such as mycorrhizal fungi is thought to be an important feedback component, yet they have shown benefits to both early and late successional plants that could either retard or accelerate succession. Here we first determine if arbuscular mycorrhizal (AM) fungi differ among three stages of primary sand dune succession and then if they alter growth of plants from particular successional stages. We isolated AM fungal inoculum from early, intermediate or late stages of a primary dune succession and compared them using cloning and sequencing. We then grew eight plant species that dominate within each of these successional stages with each AM fungal inoculum. We measured fungal growth to assess potential AM functional differences and plant growth to determine if AM fungi positively or negatively affect plants. AM fungi isolated from early succession were more phylogenetically diverse relative to intermediate and late succession while late successional fungi consistently produced more soil hyphae and arbuscules. Despite these differences, inocula from different successional stages had similar effects on the growth of all plant species. Host plant biomass was not affected by mycorrhizal inoculation relative to un-inoculated controls. Although mycorrhizal communities differ among primary dune successional stages and formed different fungal structures, these differences did not directly affect the growth of plants from different dune successional stages in our experiment and therefore may be less likely to directly contribute to plant succession in sand dunes

Light effects on seedling growth in simulated forest canopy gaps vary across species from different successional stages

Tropical forests continue to suffer from various kinds of disturbances in the Anthropocene. An immediate impact of disturbances on forest ecosystems is the creation of numerous large and small canopy gaps, which dramatically affect forest structure and function. Yet, we know little about the effect of canopy gaps on forest successional trajectory. More specifically, the responses of seedlings from different successional stages to increased light intensity under large and small canopy gaps in understory remain unclear. In this study, dominant tree seedlings from early-, mid-, and late-successional stages were selected, respectively from a tropical montane forest in Hainan Island, China to study their growth rate, biomass and traits. Our results showed that the light condition under small canopy gaps (SG, 10–15% of full sunlight) and large canopy gaps (LG, 40–50% of full sunlight) induced greater increment of relative growth rates for seedlings from early- and mid-successional stages relative to that in late-successional stage. Both SG and LG also significantly increased photosynthesis rate, leaf area (LA), light saturation point (LSP), root mass ratio (RMR) and root: shoot ratio, but decreased specific leaf area (SLA) of seedlings across successional stages. Tree seedlings from the earlysuccessional stage displayed the greatest decrease in leaf mass ratio, increase in LA, LSP, and RMR, in comparison to those from mid- and late- successional stages. Light condition and SLA were the most important factors for seedlings’ relative growth rate across successional stages. SLA connected the interaction between the light condition and successional stage on seedlings’ growth, thereby jointly explaining the 93% variation of seedlings’ growth, combining with area-based light saturated rate of CO2 assimilation. Our study highlights the distinct effect of disturbance-induced canopy gaps on seedling regeneration in the understory in tropical forest due to the variation of light intensity. We suspect that the seedlings from late-successional stage will recover relatively slow after disturbances causing canopy losses, which can have detrimental impacts on structure feature an

Microalgal species variation at different successional stages in biological soil crusts of the Gurbantunggut Desert, Northwestern China

Biological soil crusts (BSC), most notably lichen crusts, develop and diversify in the Gurbantunggut Desert, the largest fixed and semi-fixed desert in China. Four different successional stages of BSC, including bare sand, microalgal crusts, lichen crusts, and moss crusts, were selected to determine successional changes in microalgal species composition and biomass and formation of BSC. A 10 x 10-m observation plot was established in an interdune region of the Gurbantunggut Desert and data were collected over an 8-year study period. The main results were: (1) different successional stages of BSC significantly affected the content of soil organic C and total and available N but not the total and available P and K content of soil; (2) composition of microalgal communities differed among the four successional stages; (3) significant differences in microalgal biomass were observed among the four successional stages; (4) bare sand was mainly uncompacted sand gains; (5) filamentous cyanobacteria, particularly Microcoleus vaginatus, were the dominant species in the early phase of crust succession. The presence of fungal mycelium and moss rhizoids prevented water and wind erosion

Exploring approaches to improve the performance for separating successional vegetation stages in the Brazilian Amazon with remote sensing data.

The rapid growth of successional vegetation has played an important role in reducing the carbon emission to atmosphere. However, lack of spatial data sets of successional vegetation with different stages has been regarded as an important source causing the uncertainty in carbon estimation in the Amazon. The complex vegetation stand structure and associated abundant tree species often induce difficulty in vegetation classification in the moist tropical regions with remotely sensed data. With the NASA LBA-ECO funding support (1998 - 2008), we have examined successional vegetation classification in Rondonia, Brazil, through comparative analyses of different image procedures. Different sensor data, i.e., Landsat TM/ETM+, Terra ASTER, and SPOT HRG, were used. Wavelet-merging approach was used to integrate different sensor or resolution data. The roles of textures based on grey-level co-occurrence matrix were examined. Different classification approaches, such as maximum likelihood, decision tree, and ECHO were examined. Meanwhile, we explored sub-pixel based approach and a new approach based on forest stand structure. This research indicates that data fusion cannot effectively improve successional vegetation classification, but use of textures is helpful in improving classification accuracy. Overall, SPOT HRG data provided better classification accuracy than ASTER and TM/ETM+ data. However, successional vegetation classification, especially for intermediate and advanced successional vegetation stages, is still very difficult based on the direct use of remotely sensed data. Subpixel based approach improved the classification performance. In particular, the approach based on forest stand structure can successfully distinguish three successional stages (i.e., initial, intermediate, and advanced successional stages) and provide much improved classification accuracies comparing with the result from other classification approaches. These data sets will be useful for conducting the scaling-up research from local to regional successional vegetation mapping in the Amazon basin. Tipo de Apresentação: Ora

Small herbivores slow down species loss up to 22 years but only at early successional stage

The long-term influence of persistent small herbivores on successional plant community configuration is rarely studied. We used a herbivore exclusion experiment along the successional gradient in a salt-marsh system, to investigate the effects of hares and geese, and hares alone, on plant diversity at five successional stages (the earliest, two early, the intermediate and the late successional stages) in the short and long term, i.e. 7 and 22 years, respectively. Plant diversity declined over time at all successional stages except for the earliest one. Small herbivores slowed down species decline, but only at one early successional stage. Small herbivores slowed down species decline via decreasing dominance of preferred grass Festuca rubra in the short term, and less preferred Elytrigia atherica in the long term. The effects of hares and geese were more pronounced than hares alone, indicating an important additive role of geese, especially in the long term. Synthesis. Small herbivores can have a strong and long-lasting impact on plant diversity, but it highly depends on the abundance of small herbivores, which in turn depends on the quality and abundance of forage plants. A diverse herbivore community may have more positive effects on regulating plant communities

Biological significance of dead biomass retention trait in Mediterranean Basin species: an analysis between different successional niches and regeneration strategies as functional groups

Standing dead biomass retention is considered one of the most relevant fuel structural traits to affect plant flammability. However, very little is known about the biological significance of this trait and its distribution between different functional groups. Our aim was to analyse how the proportion of dead biomass produced in Mediterranean species is related to the successional niche of species (early-, mid- and late-successional stages) and the regeneration strategy of species (seeders and resprouters). We evaluated biomass distribution by size classes and standing dead biomass retention in nine dominant species from the Mediterranean Basin in different development stages (5, 9, 14 and 26 years since the last fire). The results revealed significant differences in the standing dead biomass retention of species that presented a distinct successional niche or regeneration strategy. These differences were restricted to the oldest ages studied (>9 years). Tree and small tree resprouters, typical in late-successional stages, presented slight variations with age and a less marked trend to retain dead biomass, while seeder shrubs and dwarf shrubs, characteristic of early-successional stages, showed high dead biomass loads. Our results suggest that the species that tend to retain more dead branches are colonising species that may promote fire in early-successional stages.This research has been partially financed by programme FORESTERRA ERA-Net (Medwildfirelab, PCIN-2013-140-C04-03), PROMETEO II (Desestrés/2014/038) and the Spanish Ministry of Education (Resilience CGL 2011-30515-C02-02). CEAM is supported by the Generalitat Valenciana (Regional Valencian Government)

Ant Assemblage Structure in a Secondary Tropical Dry Forest: The Role of Ecological Succession and Seasonality

This study identified the main biological mechanisms governing the diversity of ants on different ecological time scales. Ants were sampled in 15 plots distributed in early, intermediate and late stages of succession (five plots per stage) at the Parque Estadual da Mata Seca, Brazil. At each sample point, unbaited pitfall traps were installed in hypogaeic, epigaeic and arboreal strata. We collected 95 ant species from 26 genera and nine subfamilies. Our results indicated that there was an increase in species richness in advanced stages of succession. We also observed that ant assemblages were different among successional stages. For the arboreal and epigaeic strata, species richness did not change with succession progression, but species composition of these two strata differed among successional stages. Unlike to arboreal and epigaeic ants, hypogaiec ant species richness was higher in the intermediate and late stages of succession and the composition of hypogaeic ants differed among successional stages. Similarity between ant species foraging in arboreal and epigaeic strata decreases with succession progression and β-diversity was higher in advanced successional stages. Additionally, species richness was higher in the dry season, whereas the composition of ant assemblages did not change between seasons. A considerable fraction of the ant assemblage was found only in advanced stages of succession, demonstrating the importance of secondary habitats in maintaining biodiversity in dry forests

Fine-root dynamics in mixed boreal conifer-broad-leafed forest stands at different successional stages after fire

Fine-root (diameter ≤ 10 mm) standing biomass, length, distribution, production, and decomposition were studied in mixed conifer broad-leafed forest stands 48, 122, and 232 years after fire on clay soils in the southern boreal forest of Quebec. A combination of ingrowth bags, soil cores, and root litter bags was used. Forest composition changed from trembling aspen- (Populus tremuloides Michx.) dominated stands in the youngest stage to balsam fir (Abies balsamea (L.) Mill.), and then to eastern white-cedar (Thuja occidentalis L.) stands in the oldest stage. The fine- and small-root standing biomass did not differ significantly between the forest successional stages. However, the total root length was significantly more developed in the 48-year-old successional stand than in the 232-year-old stand. Within the investigated soil profile (depth = 30 cm) most roots (>80%) were found in the 5 to 8 cm thick organic layer and the top 10 cm of the mineral soil. Root biomass in the organic layer increased significantly after fire, and a simultaneous increase in thickness of the organic layer was observed. The ingrowth of roots into ingrowth bags during one and two growing seasons was more than twice as high m the youngest stand as in the oldest one. However, the differences were not statistically significant because of high variation. Fine roots of aspen decomposed significantly faster than those of balsam fir and cedar in all forest stands. The results suggest that root production, the rate of decomposition, and presumably the rate of turnover are higher in forest stands dominated by early successional broad-leafed species such as aspen than in stands composed of late successional coniferous species such as fir, spruce, and cedar. Differences in root dynamics may contribute significantly to the change in the carbon and nutrient cycling often reported with succession in the boreal forest

The use of chronosequences in studies of ecological succession and soil development

1. Chronosequences and associated space-for-time substitutions are an important and often necessary tool for studying temporal dynamics of plant communities and soil development across multiple time-scales. However, they are often used inappropriately, leading to false conclusions about ecological patterns and processes, which has prompted recent strong criticism of the approach. Here, we evaluate when chronosequences may or may not be appropriate for studying community and ecosystem development. 2. Chronosequences are appropriate to study plant succession at decadal to millennial time-scales when there is evidence that sites of different ages are following the same trajectory. They can also be reliably used to study aspects of soil development that occur between temporally linked sites over time-scales of centuries to millennia, sometimes independently of their application to shorter-term plant and soil biological communities. 3. Some characteristics of changing plant and soil biological communities (e.g. species richness, plant cover, vegetation structure, soil organic matter accumulation) are more likely to be related in a predictable and temporally linear manner than are other characteristics (e.g. species composition and abundance) and are therefore more reliably studied using a chronosequence approach. 4. Chronosequences are most appropriate for studying communities that are following convergent successional trajectories and have low biodiversity, rapid species turnover and low frequency and severity of disturbance. Chronosequences are least suitable for studying successional trajectories that are divergent, species-rich, highly disturbed or arrested in time because then there are often major difficulties in determining temporal linkages between stages. 5. Synthesis. We conclude that, when successional trajectories exceed the life span of investigators and the experimental and observational studies that they perform, temporal change can be successfully explored through the judicious use of chronosequences