Effects of synbiotic supplement on human gut microbiota, body composition and weight loss in obesity

Targeting gut microbiota with synbiotics (probiotic supplements containing prebiotic components) is emerging as a promising intervention in the comprehensive nutritional approach to reducing obesity. Weight loss resulting from low-carbohydrate high-protein diets can be significant but has also been linked to potentially negative health effects due to increased bacterial fermentation of undigested protein within the colon and subsequent changes in gut microbiota composition. Correcting obesity-induced disruption of gut microbiota with synbiotics can be more effective than supplementation with probiotics alone because prebiotic components of synbiotics support the growth and survival of positive bacteria therein. The purpose of this placebo-controlled intervention clinical trial was to evaluate the effects of a synbiotic supplement on the composition, richness and diversity of gut microbiota and associations of microbial species with body composition parameters and biomarkers of obesity in human subjects participating in a weight loss program. The probiotic component of the synbiotic used in the study contained Lactobacillus acidophilus, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium bifidum and the prebiotic component was a galactooligosaccharide mixture. The results showed no statistically significant differences in body composition (body mass, BMI, body fat mass, body fat percentage, body lean mass, and bone mineral content) between the placebo and synbiotic groups at the end of the clinical trial (3-month intervention, 20 human subjects participating in weight loss intervention based on a low-carbohydrate, high-protein, reduced energy diet). Synbiotic supplementation increased the abundance of gut bacteria associated with positive health effects, especially Bifidobacterium and Lactobacillus, and it also appeared to increase the gut microbiota richness. A decreasing trend in the gut microbiota diversity in the placebo and synbiotic groups was observed at the end of trial, which may imply the effect of the high-protein low-carbohydrate diet used in the weight loss program. Regression analysis performed to correlate abundance of species following supplementation with body composition parameters and biomarkers of obesity found an association between a decrease over time in blood glucose and an increase in Lactobacillus abundance, particularly in the synbiotic group. However, the decrease over time in body mass, BMI, waist circumstance, and body fat mass was associated with a decrease in Bifidobacterium abundance. The results obtained support the conclusion that synbiotic supplement used in this clinical trial modulates human gut microbiota by increasing abundance of potentially beneficial microbial species

Assessment of dry whey powder as prebiotic in the feeding of laying hens and broilers

Durante las últimas décadas se ha observado un incremento en la búsqueda de alternativas alimenticias diferentes a los antibióticos que mejoren el rendimiento productivo de gallinas de puesta y pollos de engorde mediante la modulación de las poblaciones microbianas del tracto gastrointestinal. Desde la perspectiva del manejo alimenticio, uno de los enfoques se centra en la inclusión de prebióticos en la dieta que actúen modulando la composición microbiana de manera beneficiosa, y por ende el estatus sanitario y productivo de los animales. De este modo, el principal objetivo de la presente tesis fue evaluar el potencial prebiótico del lactosuero en polvo (LP), como fuente de lactosa, en gallinas de puesta y pollos de engorde. Para ello se desarrollaron cinco estudios con el fin de determinar la factibilidad de incluir LP en dietas con matrices cereales de maíz-soja y trigo-cebada. Además, la presente tesis también evaluó alternativas para aumentar la efectividad del LP mediante su suplementación simultánea con otros aditivos de naturaleza no antibiótica.El primer estudio determinó el efecto de la inclusión de LP, Pediococus acidilactici (PA), y la combinación de los dos como simbiótico (DWP-PA) en dietas para gallinas ponedoras con base cereal de maíz y soja sobre el rendimiento productivo, la calidad de los huevos y los recuento microbianos cecales. Los resultados mostraron que los recuentos cecales de Bifidobacterium spp. incrementaron con la inclusion de LP. Del mismo modo, se observó una interacción entre los niveles de LP y PA en la producción de huevos y en los recuentos cecales de Clostridium perfringens, ya que la adición de LP incrementó la producción de huevos y redujo las unidades formadoras de colonia de C. perfringens sólo cuando PA no fue adicionado a la dieta. De acuerdo con estos resultados, se puede inferir que la adición de LP moduló la composición bacteriana cecal e incrementó la producción de huevos.El segundo estudio fue llevado a cabo empleando los mismos animales del primer estudio. En este caso, el objetivo fue la evaluar la composición microbiana cecal mediante la secuenciación del gen ribosomal RNA 16S, así como el perfil funcional microbiano mediante la secuenciación completa del ADN bacteriano de gallinas de puesta alimentadas con LP, PA y LP-PA. Los resultados mostraron que las comunidades microbianas de gallinas alimentadas con la dieta control y PA fueron diferentes de aquellas alimentadas con LP y LP-PA, mientras que no se observaron diferencias entre la dieta control y PA, ni entre LP y LP-PA. La suplementación de LP y LP-PA en la dieta incrementó la presencia de Olsenella spp. Lactobacillus crispatus, y Megamonas spp. en comparación con los tratamientos restantes. En relación con el perfil funcional, se observó que los todos los metagenomas bacterianos comparten un núcleo de funciones comunes (45.5%). Sin embargo, fue evidente que la adición de LP en la dieta causó que la microbiota codificara más funciones únicas (22,5%) en comparación con la dieta control, la cual mostró el porcentaje más bajo (1.6%). La dieta que contenía LP favoreció el incremento de las funciones microbianas relacionadas con el metabolismo del butanoato, propanoato, galactosa e inositol fosfato. Los resultados de este experimento indican que cada uno de los suplementos empleados influenció la comunidad microbiana cecal, pero que estos cambios no implicaron una alteración en los principales roles funcionales de la misma. Sin embargo, algunas funciones metabólicas microbianas estuvieron presentes o ausentes dependiendo de la fuente de suplementación.En el tercer estudio se evaluó la digestibilidad ileal aparente (DIA), el pH del contenido gastrointestinal de varios segmentos, la histomorfometría duodenal, los recuentos microbianos cecales y el rendimiento productivo de pollos de engorde en respuesta a la adición de LP y butirato de calcio recubierto con grasa (BCa) en dietas con base de maíz y soja. Los resultados muestran que con la adición de LP se incrementó la DIA de la materia seca, proteína cruda, Ca y P, y se redujo el pH cecal sólo cuando BCa también fue adicionado a la dieta. También observamos que la suplementación de LP incrementó la altura de las vellosidades, el ratio altura de la vellosidad: profundidad de la cripta, y el área de la superficie de las vellosidades solamente cuando BCa fue suplementado de manera conjunta, mientras que la suplementación de LP incrementó los recuentos de colonias cecales de Bifidobacterium spp. sólo cuando BCa no fue suplementado. En relación con los resultados de rendimiento productivo, se observó que la ganancia media diaria (GMD) y la ingestión de alimento (IA) incrementó durante los periodos de arranque y finalización, y durante todo el periodo de alimentación cuando LP fue suministrado de manera conjunta con BCa. Sin embargo, el índice de conversión alimenticia (ICA) se redujo con la adición de LP en ausencia de BCa y viceversa. Estos resultados sugieren que la suplementación de LP en ausencia de BCa, o de BCa en ausencia de LP mejora el ICA de los pollos de engorde. Sin embargo, la suplementación conjunta de LP y BCa mejoró el desarrollo duodenal, incrementó la digestibilidad de los nutrientes, y el peso e ingestión alimenticia de los animales. El cuarto estudio fue desarrollado con el fin de evaluar la inclusión de LP y concentrado proteico de lactosuero (CPL) en dietas para pollos de engorde con base cereal de maíz y soja, sobre la DIA, el rendimiento productivo y la composición de la microbiota cecal empleando la secuenciación del gen ribosomal RNA 16S en la plataforma de Illumina. Los resultados mostraron que la dieta 60-LP incrementó la DIA del Ca, mientras que la dieta 80-CPL mejoró la DIA del Ca y P en comparación con la dieta control. Del mismo modo, las dietas 60-L y 80-CP incrementaron el peso vivo (PV), la GMD y la IA durante los periodos de arranque, de crecimiento y finalización, y durante el periodo completo de alimentación. Las dietas 60-LP y 80-CP redujeron el ICA durante el periodo de arranque, mientras que 60-LP redujo este parámetro durante el periodo completo de alimentación. Las composición de comunidades microbianas cecales de los pollos alimentados con 60-LP y 80-CP fueron diferentes de aquellos alimentados con la dieta control. La abundancia de Bacteroides fragilis, Bacteroides spp., Escherichia coli/Shigella flexneri y Megamonas furniformis incrementó por alimentar a los animales con 60-LP y 80-CP, mientras que la presencia de Helicobacter pullorum se vio disminuida. La abundancia de Lactobacillus salivarius incrementó de manera consistente en los pollos con mayor ICA, que fueron aquellos alimentados con 60-LP. Los resultados de este estudio permiten concluir que el crecimiento de los pollos se puede mejorar por la suplementación con 60-LP y 80-CP debido a la mayor digestibilidad de los minerales, al incrementó en la IA y a la modulación en la composición de la microbiota cecal.El quinto y último estudio se realizó con el fin de investigar el efecto de suplementar 60-LP, quitosano (5-CHIT), DWP-CHIT e inulina (20-INU) en dietas para pollos de engorde con base cereal de trigo y cebada, sobre la histomorfometría duodenal, el rendimiento productivo y la composición de la microbiota cecal a los 21 y 42 días de vida, mediante la secuenciación del gen ribosomal RNA 16S en la plataforma Illumina. Los resultados de este estudio indicaron que la alimentación de los animales con cualquiera de los aditivos suministrados redujo el PV, la GMD y la IA durante el periodo de arranque. Esto también fue observado durante todo el periodo de alimentación, excepto para el tratamiento 20-INU, que mostró valores similares al grupo control. En relación a las comunidades microbianas cecales, no se observaron diferencias entre el grupo control, 60-LP, 5-QUIT y 20-INU al día 21 de vida, en los cuales el ciego estuvo principalmente colonizado por Lactobacillus gallinarum, aunque sólo el control promovió un mayor PV, GMD e IA. El grupo control y 60-LP no difirieron en sus comunidades cecales al día 42, aunque sólo el tratamiento control incrementó el PV, la GMD, y la IA. En ambos casos, el ciego tuvo una alta abundancia de Lactobacillus gallinarum and Bacteroides vulgatus, y una baja de Escherichia coli/Shigella flexneri and Bacteroides fragilis. Estos resultados indican que el crecimiento de los pollos de engorde se redujo por la suplementación de LP, QUIT, LP más QUIT e INU a las dosis evaluadas, como consecuencia de una disminución en la IA. Además, los resultados ponen en evidencia que la composición de la microbiota cecal fue modulado por la dieta en cada una de las edades muestreadas, aunque no se observó una asociación clara entre esta y el rendimiento productivoDuring the last decades, the interest to search for non-antibiotic fed alternatives to improve the performance of laying hens and broilers through gastrointestinal microbial modulations without causing antimicrobial resistance has increased considerably. From the nutritional perspective, one of the approaches is based on the dietary inclusion of prebiotics to benefit the intestinal microbial composition, and therefore animal health and performance. The main objective of the present thesis was to evaluate the prebiotic potential of the inclusion of dry whey powder (DWP), as a lactose source, in the formulation of laying hens and broilers’ feed. In this context, five studies were conducted to determine the feasibility of the use of DWP when added to corn-soybean or whey-barley based diets. Moreover, DWP effectiveness through its simultaneous supplementation with other non-antibiotic feed additives was also assessed. The first study determined the effect of supplementing corn-soybean based diets of laying hens with DWP, Pediococus acidilactici (PA), and the combination of both as synbiotic (DWP-PA) on the productive performance, egg quality traits, and cecal microbial counts. The results showed that cecal counts of Bifidobacterium spp. were increased with the addition of DWP, while an interaction between DWP and PA levels was found on egg production and on cecal counts of Clostridium perfringens, so that the addition of DWP increased egg production and reduced C. perfringens colony counts only when PA was not used. According to these results, the addition of DWP modulated the target cecal bacteria and increased egg production. The second study was carried out with the same animals used in study 1. The aim of this was to analyze the cecal microbial composition, using Illumina amplicon sequencing of the 16S rRNA gene, and the cecal microbial functional profile, using DNA sequencing through Illumina HiSeq2500 platform, of laying hens fed with DWP, PA, and DWP-PA. The results revealed that microbial communities of hens fed with control and PA were different from those fed with DWP and DWP-PA, while no differences were found between control and PA, and between DWP and DWP-PA. Feeding with DWP and DWP-PA mainly promoted the presence of Olsenella spp. Lactobacillus crispatus, and Megamonas spp. in comparison with the remaining diets. Metagenomics approach revealed that a core of main functions was shared between all metagenomes (45.5%), although DWP stimulated that microbiota encoded more unique functions (22.5%) compared with control, which showed the lowest percentage (1.6%). Major presence of genes encoding the metabolism of butanoate, propanoate, galactose, and inositol phosphate were especially stimulated by DWP. Results from this experiment indicated that each dietary supplementation influenced the cecal microbial community, but these changes did not imply a disturbance in their main biological roles. However, some specific metabolic functions encoded by the community, were present or absent depending on the source of supplementation. In the third study, apparent ileal digestibility (AID), pH of gastrointestinal content at various segments, duodenal histomorphometry, cecal microbial counts, and productive performance of broilers were studied in response to DWP and fat-coated calcium butyrate (CaB) supplementation to corn-soybean based diets. The results indicated that with the addition of DWP, the AID of dry matter, crude protein, Ca and P increased, and cecum pH decreased only when CaB was also added. Similarly, with the addition of DWP, villus height, villus height to crypt depth ratio, and villus surface area were increased only when CaB was also added, while the supplementation of WP increased Bifidobacterium spp. colony counts only when CaB was no added. In relation to performance results, it was observed that with the dietary supplementation of DWP, the average daily gain (ADG) and feed intake (FI) increased during starter-grower finisher periods, and the entire feeding period only when CaB was also added. However, with the addition of DWP, feed conversion ratio (FCR) decreased in broilers fed without CaB, but it increased in those fed with CaB during the grower-finisher and entire feeding periods. These findings suggest that the supplementation of DWP without CaB addition improve FCR of broilers. However, the joint supplementation of DWP and CaB improve duodenal development, increases nutrient AID, and the weight and feed ingestion of broilers. The fourth study was carried out to assess the influence of supplementing corn-soybean broiler diets with DWP and whey protein concentrate (WPC) on AID, productive performance and cecal microbiota composition at the end of the productive period, using Illumina amplicon sequencing of the 16S rRNA gene. The results showed that 60-DWP increased the AID of Ca, while 80-WPC improved both AID of Ca and P when compared to control diet. Feeding broilers with 60-DWP and 80-WPC increased their BW, ADG, and FI during the starter and grower-finisher periods, and during the entire feeding period. Supplementing 60-DWP and 80-WPC reduced FCR during the starter period, while 60-DWP reduced this parameter during the entire feeding period. Cecal microbial communities of broilers fed with 60-DWP and 80-WPC differed from those fed with control diet. The abundance of Bacteroides fragilis, Bacteroides spp., Escherichia coli/Shigella flexneri and Megamonas furniformis increased when 60-DWP and 80-WPC were included, while the presence of Helicobacter pullorum decreased. Lactobacillus salivarius consistently increased in chickens with better FCR, which were those fed with 60-DWP. These results indicate that growth of chickens is improved by 60-DWP and 80-WPC supplementation because of a higher mineral digestibility, increased FI, and modulation of cecal microbiota communities. The fifth study was conducted to investigate the effect of supplementing wheat-barley based diets with 60-DWP, chitosan (5-QUIT), DWP-QUIT, and Inulin (20-INU) on duodenal histomorphometry, productive performance and cecal microbiota composition at days 21 and 42 of age, using Illumina amplicon sequencing of the 16S rRNA gene. The results indicated that feeding chickens with any of the tested additives diminished their BW, ADG, and FI during the starter period. This was also observed during the entire feeding period, except for INU supplementation that showed similar values to control. At day 21, no differences in microbiota composition of control, 60-DWP, 5-CHIT and 20-INU birds were found, which ceca were highly harboured by Lactobacillus gallinarum, although only control promoted greater BW, ADG, and FI. Control and 60-DWP treatments did not differ in their ceca communities at day 42, although only control increased BW, ADG, and FI. In both cases, ceca showed higher abundance of Lactobacillus gallinarum and Bacteroides vulgatus, and lower abundance of Escherichia coli/Shigella flexneri and Bacteroides fragilis. The present findings indicate that chicken growth is reduced by supplementing wheat-barley based diets with DWP, CHIT, DWP plus CHIT, and INU, at the tested doses, as a consequence of a reduction in FI. Moreover, the results revealed that cecal microbiota composition was influenced by diet at every stage of life, although no clear association between microbiota and performance was detected. <br /

Effect of Lactobacillus casei and Garlic Powder Administration on Broiler Performance, Immune Response and Blood Profile

This study aimed to examine effect of giving a mixture of Lactobacillus casei and garlic powder (LGP) on broiler performance, immune response and blood profile. One hundred and forty-day old broilers with an average body weight of 43.70 ± 0.88 g were placed randomly in 20 experimental units. Completely randomized design was used in this study, with 4 treatments and 5 replications. The treatment was conducted when the chickens were 22-35 days old (finisher phase). The chickens were given a mixture of Lactobacillus casei and garlic powder (LGP) at concentration levels of: 0, 1, 2, and  3%. Parameters measured were blood profile, weight of lymphoid organs (bursa of Fabricius, spleen, and thymus), and carcass production. Data were analyzed for variance and if there was a significant effect, then Duncan’s multiple range test was followed at the 5% level. Results showed that the administration of LGP improved the immune response, carcass weight and final body weight of broiler chickens. The administration of 3% LGP improved immune response and achieved the best broiler performance

Effect of In ovo Injection of Probiotic, Prebiotic, and Synbiotic on Growth Performance and Gut Health Parameters of Broiler Chicken.

M.S. Thesis. University of Hawaiʻi at Mānoa 2018

Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs

Salmonellosis causes significant economic losses to the pig industry and contaminated pork products are an important source of Salmonella for humans. The EU ban on the use of antibiotic growth promoters in pig production, and the emergence of antibiotic resistance has meant there is a pressing need for alternative control strategies for pathogenic bacteria such as S. Typhimurium in pigs. Here, we determined the effects of prebiotic, probiotic and synbiotic diet regimes on antibody responses to oral Salmonella challenge of pigs. The data demonstrate that the inclusion of the probiotic Lactobacillus plantarum B2984 in the diet of piglets (∼1 × 1010 cfu/animal/day) enhanced serum IgM (P<0.001), IgG (P=0.001) and IgA (P=0.039) responses to S. Typhimurium infection including cross-reacting antibodies to S. Enteritidis. Similarly, inclusion of the prebiotic lactulose at 1% (w/w) of the feed on a daily basis in the diet enhanced serum IgM (P=0.010), IgG (P=0.004) and IgA (P=0.046) responses to S. Typhimurium infection and also cross-reacting antibodies to S. Enteritidis. Inclusion of both additives in the synbiotic diet also elicited an enhanced immune response with IgM (P=0.009) and IgG (P=0.046) levels being increased, however a significant interaction of the pre and probiotics was observed when considering the immune responses to S. Typhimurium (IgM P=0.004; IgG and IgA, P<0.001 for interaction). With respect to immune responses, the effects of pre or probiotic administration were the same or reduced in the synbiotic diet compared to when used in isolation. The data support the use of Lactobacillus plantarum B2984 or lactulose as strategies to contribute to the protection of weaned piglets from zoonotic bacterial pathogens, but caution must be taken when combining dietary supplements as combinations can interact

Investigation into the effects of probiotic, prebiotic and synbiotic feed supplements on gut microbiota, immune function and performance of broiler chickens

The aim of this project was to evaluate the effects of probiotics, prebiotics and synbiotics on the gut ecosystem, immune function and growth parameters of broiler. The first study screened naturally occurring Campylobacter levels in four local sites and revealed the NTU broiler research unit and the NTU animal unit laying hens were Campylobacter free, but a small holding with laying hens was positive and the commercial broiler farm was negative until thinning, after which it was positive. The second study investigated possible delivery routes of a novel strain of Lactobacillus johnsonii (FI9785) into broiler chicken gut and concluded feed was the optimum method for delivery. A third study compared the effect L. Johnsonii FI9785 supplied via feed to control and showed no significant difference in the CFU of caecal Campylobacter, no significant (p≤0.05) effects on growth performance and serum uric acid concentration over 4 weeks. However, mucin layer thickness in the jejunum was significantly (P≤0.05) increased. Concentration of IgA in the serum blood of probiotic treated birds was also increased but IgM and IgG were not significantly altered. Study 4 involved isolation and in vitro screening of candidate probiotic isolates of lactic acid bacteria and a prebiotic from Jerusalem artichoke plant (JA). All tests confirmed the isolates had the characteristics of lactic acid bacteria and have an inhibition activity toward Campylobacter. All isolates belonged to the genus of Lactobacillus and all retained viability during freezing and drying and the poultry gastrointestinal environment, indicating all were potential probiotic agents. Assessment of JA inulin levels indicated the plant to be a potentially good prebiotic source with these isolates. Study 5 investigated in vivo effects of the Lactobacillus isolates (probiotic), JA powder (prebiotic), synbiotic (mix of pre and probiotic). Caecal content were negative for Campylobacter throughout but at day 7, abundance of Firmicutes phyla were higher (p≤0.05) than control for all of supplements treatments and abundance of Faecalibacterium genus numerically increased in all treatments but significantly (p≤0.05) only in 5% prebiotic and probiotic supplemented diets. At day 42, abundance of genus of Erysipelotrichaceae decreased in all treatments. Assessment of growth performance showed JA had no effects but probiotic and synbiotic supplementation caused a degradation in the body weight and increased feed intake. Supplements downregulated the cytokine expression IFNγ,IL-10 and IL-6 in the ileum tissue but showed no effect in the bursa tissue

Effects of Probiotic, Prebiotic, and Synbiotic Mixed Culture Based on Wheat Pollard on Productivity of Kampung’s Chicken

This research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre)            , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60)   . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old

In ovo injection of prebiotics and synbiotics affects the digestive potency of the pancreas in growing chickens

Abstract The purpose of the study was to examine the effect of 2 prebiotics and 2 synbiotics on the digestive potency of pancreas in 1-, 3-, 7-, 14-, 21-, and 34-day-old cockerels. Prebiotics (inulin and Bi2 tos) and synbiotics (inulin + Lactococcus lactis subsp. lactis and Bi2 tos + Lactococcus lactis subsp. cremoris) were injected in ovo into the air cell on the 12th d embryonic development. Their application increased the activity of amylase, lipase, and trypsin in the pancreas. The most pronounced changes were observed at the end of the investigated rearing period (d 34). The strongest stimulative effects on amylase were shown by both synbiotics, on lipase synbiotic Bi2 tos + Lactococcus lactis subsp. cremoris, and on trypsin all the used prebiotics and synbiotics. Simultaneously, neither the absolute nor the relative mass of the pancreas in comparison to control group were changed. Also, the injected in ovo compounds did not cause a deterioration in the posthatching condition of the chicken liver, as determined by measurement of the activity of marker enzymes in the blood (alanine aminotransferase and aspartate aminotransferase). Treatment with the prebiotics and synbiotics did not change the feed conversion ratio but Bi2 tos (galacto-oligosaccharide) and inulin (fructan) + Lactococcus lactis subsp. lactis significantly increased final BW

effects of in ovo injection of prebiotics and synbiotics on the productive performance and microstructural features of the superficial pectoral muscle in broiler chickens

ABSTRACT The aim of the study was to compare the effects of 2 prebiotics and 2 synbiotics injected in ovo on productivity parameters, quality, and microstructure of the superficial pectoral muscle in 35-day-old broiler chickens. On day 12 of incubation, 9,000 eggs Ross 308 were randomly divided into 5 experimental groups treated with different bioactives in ovo injected: C, control with physiological saline; PI, with 1.760 mg inulin; PB, with 0.528 mg of commercial prebiotic Bi2 tos; SI, with 1.760 mg inulin and 1,000 CFU Lactococcus lactis spp. lactis IBB SL1; SB, with 0.528 mg Bi2 tos and 1,000 CFU Lactococcus lactis spp. cremoris IBB SC1. The synbiotic solution contained 20 μl bacterial suspension and 180 μl prebiotic solution. For productive parameters and further tests ten male birds for each experimental group were used. The birds were slaughtered on day 35 of age. At slaughter, samples of the left pectoral muscles were taken and preserved by freezing in liquid nitrogen. The pH and color of the meat were evaluated at 45 min and 24 h post-mortem. Water holding capacity (WHC) was measured and expressed as the percentage of free water in meat. Microscopic specimens were analysed using MultiScan software for the measurement of the percentage of oxidative and glycolytic fibres and mean diameter of the muscle fibres. In ovo injection of prebiotics Bi2 tos had a positive effect on body weight. In prebiotic group (PI) a negative impact on hatchability was observed. Prebiotics and synbiotics had no influence on the yield of the carcass and pectoral muscle. Bioactive compounds had a significant effect on the quality of meat parameters such as: pH 24 h (PI and PB group), L* 45' (SI and SB group), and WHC (groups PB, SI, and SB). The analysis of the enzymatic profile showed a significant increase in the percentage of glycolytic fibres in the pectoral muscle from chicken treated with a synbiotic with the addition of inulin (group SI)