Artificial light spectra and plant growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Horticultural Science in Plant Science at Massey University

The growth, development and differentiation of plants growing in natural environments is determined by biotic, genetic and physical factors. Within each plant species the absolute limits of any growth response is established by inherent genetic information and the delineation of that response is, in turn, determined by the physiology of the plant. Among the most important physical factors in any natural environment are light quality, quantity and duration. Plant growth depends on a very narrow bandwidth of the electromagnetic spectrum which usually includes the near ultraviolet (down to 320 nm), the visible, and the near infra-red (up to 800 nm) regions. The radiation of this spectral range not only supplies the necessary energy for photosynthesis on which plant metabolism is based, but also by way of various photomorphogenetic processes, it controls, independently of photosynthesis, the way in which this captured energy is directed along the various metabolic pathways. Since for most processes other than photosynthesis, the amount of radiant energy initially absorbed is low, in relation to the response effect, these light reactions can be considered to belong to a group of photostimulus processes which are characterised by dose-effect relationships. These are exothermic in that they ultimately release, or direct an amount of stored energy, which may be very large as compared with the energy content of the radiation initially responsible for the stimulus (Wassink and Stolwijk, 1956)

Development of a portable leaf photosynthesis and volatile organic compounds emission system.

Understanding how plant carbon metabolism responds to environmental variables such as light is central to understanding ecosystem carbon cycling and the production of food, biofuels, and biomaterials. Here, we couple a portable leaf photosynthesis system to an autosampler for volatile organic compounds (VOCs) to enable field observations of net photosynthesis simultaneously with emissions of VOCs as a function of light. Following sample collection, VOCs are analyzed using automated thermal desorption-gas chromatograph-mass spectrometry (TD-GC-MS). An example is presented from a banana plant in the central Amazon with a focus on the response of photosynthesis and the emissions of eight individual monoterpenes to light intensity. Our observations reveal that banana leaf emissions represent a 1.1 +/- 0.1% loss of photosynthesis by carbon. Monoterpene emissions from banana are dominated by trans-β-ocimene, which accounts for up to 57% of total monoterpene emissions at high light. We conclude that the developed system is ideal for the identification and quantification of VOC emissions from leaves in parallel with CO2 and water fluxes.The system therefore permits the analysis of biological and environmental sensitivities of carbon metabolism in leaves in remote field locations, resulting in the emission of hydrocarbons to the atmosphere.•A field-portable system is developed for the identification and quantification of VOCs from leaves in parallel with leaf physiological measurements including photosynthesis and transpiration.•The system will enable the characterization of carbon and energy allocation to the biosynthesis and emission of VOCs linked with photosynthesis (e.g. isoprene and monoterpenes) and their biological and environmental sensitivities (e.g. light, temperature, CO2).•Allow the development of more accurate mechanistic global VOC emission models linked with photosynthesis, improving our ability to predict how forests will respond to climate change. It is our hope that the presented system will contribute with critical data towards these goals across Earth's diverse tropical forests

Beneficial effects of zeolites on plant photosynthesis

A

Characteristics of C-4 photosynthesis in stems and petioles of C-3 flowering plants

Most plants are known as C-3 plants because the first product of photosynthetic CO2 fixation is a three-carbon compound. C-4 plants, which use an alternative pathway in which the first product is a four-carbon compound, have evolved independently many times and are found in at least 18 families. In addition to differences in their biochemistry, photosynthetic organs of C-4 plants show alterations in their anatomy and ultrastructure. Little is known about whether the biochemical or anatomical characteristics of C-4 photosynthesis evolved first. Here we report that tobacco, a typical C-3 plant, shows characteristics of C-4 photosynthesis in cells of stems and petioles that surround the xylem and phloem, and that these cells are supplied with carbon for photosynthesis from the vascular system and not from stomata. These photosynthetic cells possess high activities of enzymes characteristic of C-4 photosynthesis, which allow the decarboxylation of four-carbon organic acids from the xylem and phloem, thus releasing CO2 for photosynthesis. These biochemical characteristics of C-4 photosynthesis in cells around the vascular bundles of stems of C-3 plants might explain why C-4 photosynthesis has evolved independently many times

Phenotypic landscape inference reveals multiple evolutionary paths to C4_4 photosynthesis

C$_4$ photosynthesis has independently evolved from the ancestral C$_3$ pathway in at least 60 plant lineages, but, as with other complex traits, how it evolved is unclear. Here we show that the polyphyletic appearance of C$_4$ photosynthesis is associated with diverse and flexible evolutionary paths that group into four major trajectories. We conducted a meta-analysis of 18 lineages containing species that use C$_3$, C$_4$, or intermediate C$_3$-C$_4$ forms of photosynthesis to parameterise a 16-dimensional phenotypic landscape. We then developed and experimentally verified a novel Bayesian approach based on a hidden Markov model that predicts how the C$_4$ phenotype evolved. The alternative evolutionary histories underlying the appearance of C$_4$ photosynthesis were determined by ancestral lineage and initial phenotypic alterations unrelated to photosynthesis. We conclude that the order of C$_4$ trait acquisition is flexible and driven by non-photosynthetic drivers. This flexibility will have facilitated the convergent evolution of this complex trait

Palisade cell shape affects the light-induced chloroplast movements and leaf photosynthesis

Leaf photosynthesis is regulated by multiple factors that help the plant to adapt to fluctuating light conditions. Leaves of sun-light-grown plants are thicker and contain more columnar palisade cells than those of shade-grown plants. Light-induced chloroplast movements are also essential for efficient leaf photosynthesis and facilitate efficient light utilization in leaf cells. Previous studies have demonstrated that leaves of most of the sun-grown plants exhibited no or very weak chloroplast movements and could accomplish efficient photosynthesis under strong light. To examine the relationship between palisade cell shape, chloroplast movement and distribution, and leaf photosynthesis, we used an Arabidopsis thaliana mutant, angustifolia (an), which has thick leaves that contain columnar palisade cells similar to those in the sun-grown plants. In the highly columnar cells of an mutant leaves, chloroplast movements were restricted. Nevertheless, under white light condition (at 120 µmol m-2 s-1), the an mutant plants showed higher chlorophyll content per unit leaf area and, thus, higher light absorption by the leaves than the wild type, which resulted in enhanced photosynthesis per unit leaf area. Our findings indicate that coordinated regulation of leaf cell shape and chloroplast movement according to the light conditions is pivotal for efficient leaf photosynthesis

Changes in oak (Quercus robur) photosynthesis after winter moth (Operophtera brumata) herbivory are not explained by changes in chemical or structural leaf traits

Insect herbivores have the potential to change both physical and chemical traits of their host plant. Although the impacts of herbivores on their hosts have been widely studied, experiments assessing changes in multiple leaf traits or functions simultaneously are still rare. We experimentally tested whether herbivory by winter moth (Operophtera brumata) caterpillars and mechanical leaf wounding changed leaf mass per area, leaf area, leaf carbon and nitrogen content, and the concentrations of 27 polyphenol compounds on oak (Quercus robur) leaves. To investigate how potential changes in the studied traits affect leaf functioning, we related the traits to the rates of leaf photosynthesis and respiration. Overall, we did not detect any clear effects of herbivory or mechanical leaf damage on the chemical or physical leaf traits, despite clear effect of herbivory on photosynthesis. Rather, the trait variation was primarily driven by variation between individual trees. Only leaf nitrogen content and a subset of the studied polyphenol compounds correlated with photosynthesis and leaf respiration. Our results suggest that in our study system, abiotic conditions related to the growth location, variation between tree individuals, and seasonal trends in plant physiology are more important than herbivory in determining the distribution and composition of leaf chemical and structural traits

Understanding plant responses to drought: how important is woody tissue photosynthesis?

Within trees, it is known that a part of the respired CO2 is assimilated in chlorophyll-containing stem and branch tissues. However, the role of this woody tissue photosynthesis in tree functioning remains unclear, in particular under drought stress conditions. In this study, stem diameter and leaf photosynthesis were measured for one-year-old cutting-derived plants of Populus nigra 'Monviso' under both well-watered and drought stress conditions. Half of the plants were subjected to a stem and branch light-exclusion treatment to prevent woody tissue photosynthesis to occur, while the other trees served as controls. Drought stress was induced in both treatments by limiting the water supply. We found that under well-watered conditions, light-exclusion resulted in reduced stem radial daily growth rate (DG) relative to DG observed for control trees. In response to drought, stem shrinkage of the light-excluded trees was more pronounced as compared to the control trees. Maximum leaf net photosynthesis (A(max)) decreased more rapidly in light-excluded trees compared to the controls during drought stress. Our results are the first to report on the potentially significant role of woody tissue photosynthesis in tree drought stress tolerance. Moreover, our study implies that the impact of assimilation of respired CO2 on tree functioning extends beyond local stem processes and indicates that woody tissue photosynthesis is potentially a key factor in understanding plant responses to drought stress

How to Maximize Energy Content in Forage Grasses

In a recent paper, Kathryn Watts and Jerry Chatterton (2004) gave an excellent overview of the basic factors affecting carbohydrate levels in forages and how these factors affect forage management. Sugars are the substrates for all plant growth, thus, they are critical to plant growth and development. Sugars are produced by photosynthesis during daylight. At night plants use energy from sugars formed by photosynthesis to grow. Whenever the rates of photosynthesis exceed plant growth rates, carbohydrates accumulate. At times, plant stresses decrease growth rates more than photosynthesis and carbohydrates accumulate. Factors that contribute to plant stress include water and nutrient deficiencies, saline or acidic soils, as well as cold or hot temperatures. High concentrations of carbohydrates (sugars, starch, and fructan) can be found in pasture or dry hay of cool-season grasses

Photosynthetic responses of three common mosses from continental Antarctica

Predicting the effects of climate change on Antarctic terrestrial vegetation requires a better knowledge of the ecophysiology of common moss species. In this paper we provide a comprehensive matrix for photosynthesis and major environmental parameters for three dominant Antarctic moss species (Bryum subrotundifolium, B. pseudotriquetrum and Ceratodon purpureus). Using locations in southern Victoria Land, (Granite Harbour, 77°S) and northern Victoria Land (Cape Hallett, 72°S) we determined the responses of net photosynthesis and dark respiration to thallus water content, thallus temperature, photosynthetic photon flux densities and CO2 concentration over several summer seasons. The studies also included microclimate recordings at all sites where the research was carried out in field laboratories. Plant temperature was influenced predominantly by the water regime at the site with dry mosses being warmer. Optimal temperatures for net photosynthesis were 13.7°C, 12.0°C and 6.6°C for B. subrotundifolium, B. pseudotriquetrum and C. purpureus, respectively and fall within the known range for Antarctic mosses. Maximal net photosynthesis at 10°C ranked as B. subrotundifolium > B. pseudotriquetrum > C. purpureus. Net photosynthesis was strongly depressed at subzero temperatures but was substantial at 0°C. Net photosynthesis of the mosses was not saturated by light at optimal water content and thallus temperature. Response of net photosynthesis to increase in water content was as expected for mosses although B. subrotundifolium showed a large depression (60%) at the highest hydrations. Net photosynthesis of both B. subrotundifolium and B. pseudotriquetrum showed a large response to increase in CO2 concentration and this rose with increase in temperature; saturation was not reached for B. pseudotriquetrum at 20°C. There was a high level of variability for species at the same sites in different years and between different locations. This was substantial enough to make prediction of the effects of climate change very difficult at the moment