Antioxidant activity, photosynthetic rate, and Spectral mass in bean Plants (Phaseolus vulgaris L.) in Response to Stress Defense Activators

An increase in antioxidant activity is a common response in plants as a defense mechanism against biotic and abiotic stress factors, such response is also generated with the exogenous application of "defense activators", which have negative effects on plant metabolism. In this work, bean plants (Phaseolus vulgaris L.) cv. Pinto Nacional were treated with jasmonic acid (0.5 mM), salicylic acid (2 mM), Trichoderma asperellum (105 spores/ml), and Bacillus pumilus (105 CFU / mL), in order to determine the level of structural and metabolic response of the plants. On the seventh day after the application of the treatments, it was measured the enzymatic activity of catalase (CAT), peroxidase (POX), and superoxide dismutase (SOD). In addition, leaf impressions were taken to measure the stomatal opening and conductance, photosynthetic rate, and the mass spectrum (mass/charge, m/z). The antioxidant activity increased in plants treated with jasmonic acid and T. asperellum, which in turn significantly increased the stomatal opening and conductance, and photosynthetic rate. The mass profile showed that the plants treated with T. asperellum have a greater quantity of masses/charge, of which some had statistically highly significant difference according to the means test Tukey (

Some physiological effects of two algal epiphytes on the surfgrass Phyllospadix torreyi Wats.

Measurement of the physiological effects of the red algal epiphytes Smithora naiadum (Anders.) Hollenberg and Melobesia mediocris (Fosl.) Setch. and Mason on Phyllospadix torreyi Wats. were made near Hopkins Marine Station, Pacific Grove, California. Field studies revealed a significant influence of these epiphytes on both the breakage incidence and length, with that of Melobesia being the most pronounced. Analysis of the photosynthetic rate of the seagrass shows a decrease in the photosynthetic maxima in both epiphytized samples at a light saturating intensity. Under light limiting conditions, an increase in photosynthetic efficiency and a change in chlorophyll a composition in both epiphytized sample types suggest an adaptive mechanism similiar to those found in terrestrial and aquatic shade plants

Modification of light utilization for skeletal growth by water flow in the scleractinian coral Galaxea fascicularis

In this study, we tested the hypothesis that the importance of water flow for skeletal growth (rate) becomes higher with increasing irradiance levels (i.e. a synergistic effect) and that such effect is mediated by a water flow modulated effect on net photosynthesis. Four series of nine nubbins of G. fascicularis were grown at either high (600 µE m-2 s-1) or intermediate (300 µE m-2 s-1) irradiance in combination with either high (15–25 cm s-1) or low (5–10 cm s-1) flow. Growth was measured as buoyant weight and surface area. Photosynthetic rates were measured at each coral’s specific experimental irradiance and flow speed. Additionally, the instantaneous effect of water flow on net photosynthetic rate was determined in short-term incubations in a respirometric flowcell. A significant interaction was found between irradiance and water flow for the increase in buoyant weight, the increase in surface area, and specific skeletal growth rate, indicating that flow velocity becomes more important for coral growth with increasing irradiance levels. Enhancement of coral growth with increasing water flow can be explained by increased net photosynthetic rates. Additionally, the need for costly photo-protective mechanisms at low flow regimes could explain the differences in growth with flow

Is whole-plant photosynthetic rate proportional to leaf area? A test of scalings and a logistic equation by leaf demography census.

application/pdfAllometric scalings and a logistic equation assume that whole-plant photosynthetic rate under resource-unlimited conditions is proportional to leaf area. We tested this proportionality for the herb Helianthus tuberosus. During growth, we repeatedly measured the percentage of leaves with high, medium, and low photosynthetic capacity to estimate the whole-plant sum of photosynthetic capacity. We found that the whole-plant sum of the light-saturated photosynthetic rate of leaves is proportional to the whole-plant leaf area, disregarding the dynamics of the leaf population. We also found that the daily photosynthesis of each leaf appeared as a linear function of the light-saturated photosynthetic rate of that leaf, as predicted by the optimization theory. Using those results, we expressed whole-plant photosynthetic rate as a product of the light-saturated whole-plant photosynthetic rate and an efficiency index that reflects resource limitation as in the logistic equation. This efficiency decreased with increasing leaf area, reflecting light limitation. Therefore, realized whole-plant photosynthetic rate is not proportional to leaf area. These "diminishing returns" are well explained by a simple saturating curve, such as the logistic equation.journal articl

Effects of CO2 enrichment on photosynthesis, growth, and nitrogen metabolism of the seagrass Zostera noltii

Seagrass ecosystems are expected to benefit from the global increase in CO2 in the ocean because the photosynthetic rate of these plants may be C-i-limited at the current CO2 level. As well, it is expected that lower external pH will facilitate the nitrate uptake of seagrasses if nitrate is cotransported with H+ across the membrane as in terrestrial plants. Here, we investigate the effects of CO2 enrichment on both carbon and nitrogen metabolism of the seagrass Zostera noltii in a mesocosm experiment where plants were exposed for 5 months to two experimental CO2 concentrations (360 and 700 ppm). Both the maximum photosynthetic rate (Pm) and photosynthetic efficiency (alpha) were higher (1.3- and 4.1-fold, respectively) in plants exposed to CO2-enriched conditions. On the other hand, no significant effects of CO2 enrichment on leaf growth rates were observed, probably due to nitrogen limitation as revealed by the low nitrogen content of leaves. The leaf ammonium uptake rate and glutamine synthetase activity were not significantly affected by increased CO2 concentrations. On the other hand, the leaf nitrate uptake rate of plants exposed to CO2-enriched conditions was fourfold lower than the uptake of plants exposed to current CO2 level, suggesting that in the seagrass Z. noltii nitrate is not cotransported with H+ as in terrestrial plants. In contrast, the activity of nitrate reductase was threefold higher in plant leaves grown at high-CO2 concentrations. Our results suggest that the global effects of CO2 on seagrass production may be spatially heterogeneous and depend on the specific nitrogen availability of each system. Under a CO2 increase scenario, the natural levels of nutrients will probably become limiting for Z. noltii. This potential limitation becomes more relevant because the expected positive effect of CO2 increase on nitrate uptake rate was not confirmed.Fundacao para a Ciencia e a Tecnologia [SFRH/BD/21487/2005]; POCI; FSE; COST; EC; EUinfo:eu-repo/semantics/publishedVersio

Cytochrome cM decreases photosynthesis under photomixotrophy in Synechocystis sp. PCC 6803

Photomixotrophy is a metabolic state that enables photosynthetic microorganisms to simultaneously perform photosynthesis and metabolism of imported organic carbon substrates. This process is complicated in cyanobacteria, since many, including Synechocystis sp. PCC 6803, conduct photosynthesis and respiration in an interlinked thylakoid membrane electron transport chain. Under photomixotrophy, the cell must therefore tightly regulate electron fluxes from photosynthetic and respiratory complexes. In this study, we demonstrate, via characterization of photosynthetic apparatus and the proteome, that photomixotrophic growth results in a gradual inhibition of QA- reoxidation in wild-type Synechocystis, which largely decreases photosynthesis over 3 d of growth. This process is circumvented by deleting the gene encoding cytochrome cM (CytM), a cryptic c-type heme protein widespread in cyanobacteria. The ΔCytM strain maintained active photosynthesis over the 3-d period, demonstrated by high photosynthetic O2 and CO2 fluxes and effective yields of PSI and PSII. Overall, this resulted in a higher growth rate compared to that of the wild type, which was maintained by accumulation of proteins involved in phosphate and metal uptake, and cofactor biosynthetic enzymes. While the exact role of CytM has not been determined, a mutant deficient in the thylakoid-localized respiratory terminal oxidases and CytM (ΔCox/Cyd/CytM) displayed a phenotype similar to that of ΔCytM under photomixotrophy. This, in combination with other physiological data, and in contrast to a previous hypothesis, suggests that CytM does not transfer electrons to these complexes. In summary, our data suggest that CytM may have a regulatory role in photomixotrophy by modulating the photosynthetic capacity of cells

Photosynthetic performance of Xanthoria mawsonii C. W. Dodge in coastal habitats, Ross Sea region, continental Antarctica

Xanthoria mawsonii C. W. Dodge was found to perform well physiologically in a variety of habitats at high latitudes in continental Antarctica. The net photosynthetic rate of 7•5 μ mol CO2 kg−1 s−1 is exceptionally high for Antarctic lichens. Field and laboratory measurements proved the photosynthetic apparatus to be highly adapted to strong irradiance. The cold resistance of the photosystem II reaction centres is higher than the photosynthetic CO2 fixation process. Optimum temperature for net photosynthesis was c. 10°C. The lichen grows along water channels where it is frequently inundated and hydrated to maximum water content, although net photosynthesis is strongly depressed by super saturation. In these habitats the lichen is photosynthetically active for long periods of time. Xanthoria mawsonii also grows at sites where it depends entirely on the early spring snow melt and occasional snow fall for moisture. It has an exceptionally short reactivation phase and is able to utilize snow immediately. Recovery of activity by absorbing water vapour from air, though practically possible, seems to be of ecological importance only under snow at subzero temperatures

Effects of canopy closure on photosynthetic characteristics of Ilex latifolia Thunb. in Phyllostachys pubescens forests

Plantation under the forest is a good way of agroforestry, but the canopy closure has a great influence on understory herbs’ growth. In the study, different canopy closures of Phyllostachys pubescens forests were set up to explore its influence on the growth of Ilex latifolia Thunb. The photosynthetic characteristics of Ilex latifolia leaves under different canopy closures were determined by Li-6400 portable photosynthetic system. The results showed that the net photosynthetic rate curve of Ilex latifolia leaves of T1 (canopy closure of 0.56) was bimodal with an obvious "midday depression" phenomenon, while the net photosynthetic rate curves of T2 (canopy closure of 0.72) and T3 (canopy closure of 0.86) were unimodal. The results of light response curve showed that the photosynthetically active radiation and transpiration rate reduced with the increasing of canopy closures. The photosynthetically active radiation, transpiration rate, stomatal conductance, and net photosynthetic rate of Ilex latifolia leaves of T2 were higher than those of T3. Although the net photosynthetic rate of T2 was lower than that of T1, it had no obvious photo-inhibition which affected plant growth. Overall, the canopy closure of 0.72 was more suitable for the growth of Ilex latifolia. The herb plantation in the bamboo forest should be considered with the canopy closure for a better growth

Isoprene Emission and Carbon Dioxide Protect Aspen Leaves from Heat Stress

High temperature, especially above 35oC, is known to reduce leaf photosynthetic rate in many tree species. This study investigated the effect of high temperature on isoprene-emitting (aspen) and non- emitting (birch) trees under ambient and elevated CO2 under open field conditions. Aspen trees tolerate heat better than birch trees and elevated CO2 protects both species against moderate heat stress. The increased thermotolerance in aspen trees compared to the birch trees may result from the aspen's ability to produce isoprene. Elevated CO2 increased carboxylation capacity, photosynthetic electron transport capacity and triose phosphate use in both birch and aspen trees. High temperature decreased all of these parameters in birch regardless of CO2 treatment but only photosynthetic electron transport and triose phosphate use at ambient CO2 were reduced in aspen. As temperature rises, non-isoprene-emitting trees will be at a disadvantage and biological diversity and species richness might be lost in some ecosystems. Our results indicate that isoprene emitting tree species will have an advantage over non-isoprene emitting ones under high temperatures