Dengue Virus Targets the Adaptor Protein MITA to Subvert Host Innate Immunity

Dengue is one of the most important arboviral diseases caused by infection of four serotypes of dengue virus (DEN). We found that activation of interferon regulatory factor 3 (IRF3) triggered by viral infection and by foreign DNA and RNA stimulation was blocked by DEN-encoded NS2B3 through a protease-dependent mechanism. The key adaptor protein in type I interferon pathway, human mediator of IRF3 activation (MITA) but not the murine homologue MPYS, was cleaved in cells infected with DEN-1 or DEN-2 and with expression of the enzymatically active protease NS2B3. The cleavage site of MITA was mapped to LRR↓96G and the function of MITA was suppressed by dengue protease. DEN replication was reduced with overexpression of MPYS but not with MITA, while DEN replication was enhanced by MPYS knockdown, indicating an antiviral role of MITA/MPYS against DEN infection. The involvement of MITA in DEN-triggered innate immune response was evidenced by reduction of IRF3 activation and IFN induction in cells with MITA knockdown upon DEN-2 infection. NS2B3 physically interacted with MITA, and the interaction and cleavage of MITA could be further enhanced by poly(dA:dT) stimulation. Thus, we identified MITA as a novel host target of DEN protease and provide the molecular mechanism of how DEN subverts the host innate immunity

Application of response surface methodology for the optimization of supercritical CO2 extraction of oil from olive paste: yield, content of bioactive molecules and biological effects in vivo

none8openLenucci MS, Durante M, Ferramosca A, Zara V, Piro G, Bergamo P, Treppiccione L, Mita GLenucci, Ms; Durante, M; Ferramosca, A; Zara, V; Piro, G; Bergamo, P; Treppiccione, L; Mita,

Technological strategies for the use of watermelon as a source of bioactive molecules and antioxidant pigments

none8openLenucci MS, Grassi S, Marrese PP, Montefusco A, De Caroli M, Durante M, Mita G, Piro GLenucci, Ms; Grassi, S; Marrese, Pp; Montefusco, A; De Caroli, M; Durante, M; Mita, G; Piro,

Analysis of Factors that Influence Consumers’ Trust to Use AI Service: The Case of Mandiri Bank’s MITA Chatbot

The use of chatbots has seen rapid development across various sectors in recent years, including in the banking industry. This study evaluated and examined the relationship between factors that influence consumer trust in the use of the AI chatbot service, MITA, at Mandiri Bank. The importance of conducting this research lies in answering questions regarding the key factors that affect users' or customers' trust in chatbots, such as perceived benefits and ease of use, which influence the intention to adopt new technologies, particularly Mandiri Bank’s MITA services. A total of 266 survey samples were collected from users of the Mandiri Bank’s MITA chatbot via an online survey, using convenience sampling for sample selection. The statistical analysis technique used in this study was Partial Least Squares Structural Equation Modeling (PLS-SEM) through the SmartPLS software. This study shows that perceived usefulness and user comfort do not have a significant effect on user trust in Mandiri Bank’s MITA chatbot. However, other factors positively and significantly affected user trust in the MITA chatbot. This study is expected to encourage marketing managers to explore more broadly and deeply how to build user trust in the banking industry and its future implication

“A Kaleidoscope of Color or the Agony of Race? Barack Obama’s Dreams from My Father”

Excerpt from Developing Transnational American Studies, edited by Nadja Gernalzick and Heike C. Spickerman

CULTURAL TRANSLATION OF TRADITIONAL FOOD FROM WEST-JAVA: A MEDIA TO PROMOTE THE TRADITIONAL CUISINE TO THE WORLD

Indonesia is the largest archipelagic country in the world with a total of 17,054 islands. Each island has different customs, values and habits. As one of the elements of culture,Indonesian culinary is also a pride cultural element. Traditional Indonesian food is one ofthe attractions of foreign tourists and local tourists. In Indonesia there are many kinds oftraditional food. From 17,054 islands that make up the Indonesian archipelago, there is theisland of Java which is known for its unique history and its beauty. It is named WestJava.West Java Province, also known as the land of Sunda, has different kind of dishescomparing to other Indonesian cuisine. Sundanese people by utilizing the existing ingredients in the surrounding likes to create a unique food in terms of names and flavors.Name it colenak, combro, batagor, cireng, cimol, gehu, and so on. In translation, there isprocedure known as cultural equivalent. Cultural equivalent is an approximate translationwhere a source language of cultural word is translated by a target language cultural word(Newmark, 1988:82). The translation from kinds of food from West Java is a way topromote the traditional food to international world. The research is conducted usingdescriptive- qualitative method. In this study, the object of the research data is the typicalfood of West Java which is located in big cities in West Java, such as in Sumedang andGarut. The data are obtained by conducting survey method and questionnaire interview. The researcher goes to the area to capture the culinary trademarks in some areas in WestJava. The results of this research will give big effect to tourism development in West Java cuisine

Delegation, Knowledge Integration, and Cooperation: How to Solve Problems of Coordination in Structural Fund Programs. Findings From Comparative Case Studies in the South of Italy

European cohesion policies are increasingly relying on grassroots networks tapping into tacit knowledge and participatory decision-making processes. Regional governments delegate their decision making power to local institutions with the assumption that local agents possess both contextual knowledge and political legitimacy to integrate different policy measures in a cooperative fashion. Delegation of decision making power is therefore presumed to minimize the unintended or conflicting outcomes emerging, for instance, when environmental protection and infrastructure building are not designed consistently to local contextual needs nor are these pursued through a cooperative effort of local networks of actors. Different agents, including resource users and government agencies try to work together to resolve shared dilemmas of coordination, as an increasingly common alternative to centralized institutions. Coordination consists of managing interdependencies among multiple individuals or organizations involved in the overall program or project management. Several studies classify different types of coordination mechanisms, including standards, hierarchy, targets or plans, slack resources, vertical information systems, direct contact, liaison roles, task forces, and integrating roles. Other ways of classifying coordination include formal impersonal, formal interpersonal, and informal interpersonal; non-coordination, standards, schedules and plans, mutual adjustment, and teams; task-task, task-resource, and resource-resource coordination; vertical and horizontal coordination; coordination by programming and by feedback; and coordination by standards, plans, and mutual adjustment. Building upon a current field research in four regions of the South of Italy, this paper examines how coordination occurs across local development programs, which are embedded within multilevel governance structures and relations. The paper presents a number of cases of local collaborations in which large numbers of local actors representing a wide range of contending groups have, with the help of mediating institutions, worked out agreements for integrating development programs. In some circumstances, specific coordination mechanisms encouraged consensus building offering all relevant groups the knowledge and skills needed to participate in these negotiations. In other circumstances, though, delegation of decision making power opened the door for opportunistic participation, lacking vision and trust for mutual cooperation.

Muski, Phrygiërs en koning Midas

The aim of this article is to discuss the thesis that the Muski and the Phrygians are the same people and that king Midas is identical to king Mita. The Muski are known only from Iron Age Assyrian chronicles. The oldest documents concerning the Muski show that they lived in northern Mesopotamia around 1165 BC. They flourished under the reign of Sargon II in the late 8th century BC, when their king Mita undertook military campaigns in the region of Neo-Hittite Tabal and Que. The Phrygians, in turn, find their origin in the Balkan. After their migration to Asia, possibly recorded in archaeology, they can be found as easterly as Hattuša and Tuwana. From an inscription of Midas City we know that Midas must be dated in the same period as Mita. It is remarkable that archaeology places the Phrygians in the same region where the Muski are located according to Assyrian documents. It would be strange that the Assyrians knew about the Muski, but were unaware of the Phrygians. Therefore, it can be argued that the Muski and the Phrygians were actually the same people. If this hypothesis is correct, then they possibly intermixed at some point in history, perhaps around the period of Mita/Midas. Nevertheless, due to the fragmentary state of the sources, a lot of uncertainty and speculation remains