The next widespread bamboo flowering poses a massive risk to the giant panda

The IUCN Red List has downgraded several species from “endangered” to “vulnerable” that still have largely unknown extinction risks. We consider one of those downgraded species, the giant panda, a bamboo specialist. Massive bamboo flowering could be a natural disaster for giant pandas. Using scenario analysis, we explored possible impacts of the next bamboo flowering in the Qinling and Minshan Mountains that are home to most giant pandas. Our results showed that the Qinling Mountains could experience large-scale bamboo flowering leading to a high risk of widespread food shortages for the giant pandas by 2020. The Minshan Mountains could similarly experience a large-scale bamboo flowering with a high risk for giant pandas between 2020 and 2030 without suitable alternative habitat in the surrounding areas. These scenarios highlight thus-far unforeseen dangers of conserving giant pandas in a fragmented habitat. We recommend advance measures to protect giant panda from severe population crashes when flowering happens. This study also suggests the need to anticipate and manage long-term risks to other downgraded species

Observing giant panda habitat and forage abundance from space

Giant pandas are obligate bamboo grazers. The bamboos favoured by giant pandas are typical forest understorey plants. Therefore, the availability and abundance of understorey bamboo is a key factor in determining the quantity and quality of giant panda food resources. However, there is little or no information about the spatial distribution or abundance of bamboo underneath the forest canopy, due to the limitations of traditional ground survey and remote sensing classification techniques. In this regard, the development of methods that can predict the understorey bamboo spatial distribution and cover abundance is critical for an improved understanding of the habitat, foraging behaviour and distribution of giant pandas, as well as facilitating an optimal conservation strategy for this endangered species. The objectives of this study were to develop innovative methods in remote sensing and GIS for estimating the giant panda habitat and forage abundance, and to explain the altitudinal migration and the spatial distribution of giant pandas in the fragmented forest landscape. It was concluded that 1) the vegetation indices derived from winter (leaf-off) satellite images can be successfully used to predict the distribution of evergreen understorey bamboo in a deciduous-dominated forest, 2) winter is the optimal season for quantifying the coverage of evergreen understorey bamboo in a mixed temperate forest, regardless of the classification methods used, 3) a higher mapping accuracy for understorey bamboo in a coniferous-dominated forest can be achieved by using an integrated neural network and expert system algorithm, 4) the altitudinal migration patterns of sympatric giant pandas and golden takins are related to satellite-derived plant phenology (a surrogate of food quality) and bamboo abundance (a surrogate of food quantity), 5) the driving force behind the seasonal vertical migration of giant pandas is the occurrence of bamboo shoots and the temperature variation along an altitudinal gradient, 6) the satellite-derived forest patches occupied by giant pandas were significantly larger and more contiguous than patches where giant pandas were not recorded, indicating that giant pandas appear sensitive to patch size and isolation effects associated with forest fragmentation. Overall, the study has been shown the potential of satellite remote sensing to map giant panda habitat and forage (i.e., understorey bamboo) abundance. The results are important for understanding the foraging behaviour and the spatial distribution of giant pandas, as well as the evaluation and modelling of giant panda habitat in order to guide decision-making on giant panda conservation. <br/

Chemical Signaling by Giant Pandas to Communicate Sexual Receptivity

As solitary animals, giant pandas (Ailuropoda melanoleuca) rely on chemical communication in order to determine reproductive condition of conspecifics. Therefore, we hypothesize certain biogenic volatile compounds affect mating behavior in giant pandas. Giant pandas housed at Memphis Zoo, Zoo Atlanta, San Diego Zoo, Edinburgh Zoo, and Toronto Zoo (n=5 males and n=5 females) were the subjects of this study. Urine, anatomical site, and environmental samples were collected during the breeding period (Feb-June) and non-breeding period (Aug-Dec) from 2012-2016. Volatile compounds in urine and the environment were extracted using solid phase micro-extraction (SPME), while compounds collected from various body sites of giant pandas were extracted with hexane. Compounds were analyzed and identified using gas chromatography mass spectrometry (GC-MS). Male behavioral trials consisted of a simultaneous choice test between days of the estrous cycle (e.g. proestrus, estrus, and metestrus) and pooled diestrus urine of unknown female giant pandas. The experimental period included eight 15-minute behavior trials over the course of 12 days. In addition, male giant pandas were exposed to isolated compounds found naturally in female urine during four 15-minute behavior trials over the course of 7 days. We predict that urine from a specific day of the peri-estrual period motivates sexual behaviors and physiological responses in males. Male physiological responses to female urine and specific chemical compounds were examined by changes in urinary androgen metabolites and the urinary volatile profile. By coupling male behavior responses and the female urinary chemical profile during the peri-estrual period, relevant compounds may be identified as possible pheromones related to estrus and mating behavior. To increase genetic viability of small populations, giant pandas require continued conservation and management aimed at facilitating communication and breeding across isolated populations. With low population numbers of giant pandas in the wild spread across fragmented habitats, a better knowledge of chemical communication in this species may provide vital information to improve the conservation and management of giant pandas

Panda Diplomacy: China\u27s Use of Soft Power to Influence the World

Although giant pandas lack a long-standing history in Imperial China, they hold a strong connection to the Chinese Communist Party and Chinese Nationalism in the modern day. This research attempts to answer the question of whether giant pandas are an influential tool of soft power and achieve the purpose of promoting a “softer” image of China globally. Through a literature review and the method of a survey, research revealed that giant pandas are a successful tool of soft power. Overall animals serve a larger purpose in policy than simply a pet object. They can influence people and nations

USING AILUROPODA MELANOLEUCA AS A MODEL SPECIES FOR STUDYING THE ECOMORPHOLOGY OF PARANTHROPUS

Giant pandas (Ailuropoda melanoleuca) have cranial morphology similar to the extinct hominin genus Paranthropus which makes them an excellent model species when studying Paranthropus diet. Both species have wide skulls with flared zygomatic arches adapted for chewing. To gain insight into possible food sources of Paranthropus, I investigated the giant panda’s specialized diet of bamboo. The toughness, hardness, and stiffness of various bamboo species was determined to assess mechanical challenges facing giant pandas during feeding. Bamboo is thought to be tough, but studies on such properties and how they apply to mastication of giant pandas are largely absent from the scientific literature. Knowing the properties of bamboo will help draw a parallel between giant panda and Paranthropus diets. Mechanical properties data were gathered from young and adult bamboo shoots using a universal testing machine, which applies and measures force to the bamboo samples. A collection of four species, which include bamboo favored and ignored by giant pandas, were tested to determine how bamboo properties vary interspecifically with the goal of discovering if there are mechanical differences between bamboo favored and disliked by the species. Conducting this research will aid efforts to understand the diet of Paranthropus and could help establish a link between Paranthropus and a food source with properties comparable to those of bamboo

Prolonged transition time between colostrum and mature milk in a bear, the giant panda, Ailuropoda melanoleuca

Bears produce the most altricial neonates of any placental mammal. We hypothesized that the transition from colostrum to mature milk in bears reflects a temporal and biochemical adaptation for altricial development and immune protection. Comparison of bear milks with milks of other eutherians yielded distinctive protein profiles. Proteomic and metabolomic analysis of serial milk samples collected from six giant pandas showed a prolonged transition from colostrum to main-phase lactation over approximately 30 days. Particularly striking are the persistence or sequential appearance of adaptive and innate immune factors. The endurance of immunoglobulin G suggests an unusual duration of trans-intestinal absorption of maternal antibodies, and is potentially relevant to the underdeveloped lymphoid system of giant panda neonates. Levels of certain milk oligosaccharides known to exert anti-microbial activities and/or that are conducive to the development of neonatal gut microbiomes underwent an almost complete changeover around days 20–30 postpartum, coincident with the maturation of the protein profile. A potential metabolic marker of starvation was detected, the prominence of which may reflect the natural postpartum period of anorexia in giant panda mothers. Early lactation in giant pandas, and possibly in other ursids, appears to be adapted for the unique requirements of unusually altricial eutherian neonates

The effect of diffusion on giant pandas that live in complex patchy environments

The habitat loss and fragmentation is almost the greatest threat to the survival of the wild giant panda. In this paper, we construct a mathematical model to consider the effect of diffusion on giant pandas that live in complex patchy environments. Our discussion includes the studying of a diffusive n-dimensional single species model, sufficient conditions are derived for the permanence and extinction of the giant panda species. Especially, we also discuss the situations of diffusion of giant pandas between two patches, and numerical simulations are presented to illustrate the results. Furthermore, we consider the existence, uniqueness, and global stability of the positive periodic solution of the n-dimensional single species model. The implications of these results are significant for giant panda conservation

First Report of a Novel Hepatozoon sp. in Giant Pandas (Ailuropoda melanoleuca)

The first report of giant pandas (Ailuropoda melanoleuca) infected with a novel Hepatozoon species is presented. An intraleukocytic parasite was detected via routine blood smear from a zoo-housed giant panda at the National Zoological Park. Ribosomal DNA sequences indicated a previously undescribed Hepatozoon species. Phylogenetic and distance analyses of the sequences placed it within its own branch, clustered with Old World species with carnivore (primarily ursid and mustelid) hosts. Retrospective and opportunistic testing of other individuals produced additional positive detections (17/23, 73.9%), demonstrating 100% prevalence (14/14) across five institutions. All animals were asymptomatic at time of sampling, and health implications for giant pandas remain unknown

Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey

Gram-negative bacteria are major pathogens that can cause illnesses in giant pandas. Lipopolysaccharides (LPS), components of Gram-negative bacteria, can activate immune responses in mammals (i.e., humans and mice) through recognition by toll-like receptors (TLRs). However, the giant pandas’ immune response to LPS stimulation and the differences between the giant panda and other mammals are not fully known. In this study, we administrated peripheral blood mononuclear cells (PBMCs) from giant pandas, humans, C57BL/6 mice, and rhesus monkeys by LPS treatment at 6 h followed by RNA sequencing (RNA-seq), respectively, with control of non-stimulation. KEGG analyses of differentially expressed genes (DEGs) pathways indicated that LPS could activate the classic signaling pathway of NF-κB in PBMCs from those four tested species. Thus, similar to the other three species, NF-κB is an LPS-responsive regulator of innate immune responses in giant pandas. Furthermore, the expression patterns of adapter genes, inflammatory cytokine genes, chemokines, interferon genes, cytokine genes related to cell growth and development, costimulatory molecules, Th1/Th2 cytokine genes, Th17 cytokine genes, Th9, and Th22 cytokine genes were compared among giant pandas and three other species. Our data indicated that in addition to the similar expression patterns of certain genes among giant pandas and other species, the unique expression pattern response to LPS in giant pandas was also discovered. Furthermore, Th9, Th17, and Th22 cells might be involved in the response to LPS in giant pandas at this tested time point. This study reveals that LPS-induced immune responses have different sensitivities and response timelines in giant pandas compared with other mammals. This study facilitates further understanding of the role of the TLR signaling pathway and the immune system in giant pandas, which might be helpful for disease prevention and protection

Changes in the milk metabolome of the Giant Panda (Ailuropoda melanoleuca) with time after birth: three phases in early lactation and progressive individual differences

Ursids (bears) in general, and giant pandas in particular, are highly altricial at birth. The components of bear milks and their changes with time may be uniquely adapted to nourish relatively immature neonates, protect them from pathogens, and support the maturation of neonatal digestive physiology. Serial milk samples collected from three giant pandas in early lactation were subjected to untargeted metabolite profiling and multivariate analysis. Changes in milk metabolites with time after birth were analysed by Principal Component Analysis, Hierarchical Cluster Analysis and further supported by Orthogonal Partial Least Square-Discriminant Analysis, revealing three phases of milk maturation: days 1–6 (Phase 1), days 7–20 (Phase 2), and beyond day 20 (Phase 3). While the compositions of Phase 1 milks were essentially indistinguishable among individuals, divergences emerged during the second week of lactation. OPLS regression analysis positioned against the growth rate of one cub tentatively inferred a correlation with changes in the abundance of a trisaccharide, isoglobotriose, previously observed to be a major oligosaccharide in ursid milks. Three artificial milk formulae used to feed giant panda cubs were also analysed, and were found to differ markedly in component content from natural panda milk. These findings have implications for the dependence of the ontogeny of all species of bears, and potentially other members of the Carnivora and beyond, on the complexity and sequential changes in maternal provision of micrometabolites in the immediate period after birth