Supplementary document for Deconvolution Stimulated Raman Scattering Microscopy Enhanced by Quantum Light - 6718930.pdf

Supplementary Documen

Bessel Beam Beating-Based Spontaneous Raman Tomography Enables High-Contrast Deep Tissue Raman Measurements

We report on the development of a novel Bessel beam beating-based spontaneous Raman tomography (B3-SRT) technique for depth-resolved deep tissue Raman characterization without the need for mechanical z-scanning. The tissue Raman signal is successfully modulated longitudinally for the first time by B3-SRT for depth information encoding and retrieval of the tissue Raman signal. To accomplish B3-SRT, we conceived a unique method by designing a coaxial Bessel beam dynamic beating excitation associated with the Bessel-shaped collection scheme, such that the depth-resolved Raman information is encoded by the Bessel beam dynamic beating generated, and then the depth-encoded Raman spectra along the Bessel beam excitation region are collected simultaneously by a Bessel-shaped collection optical design. Depth-resolved Raman spectra can be rapidly retrieved using inverse fast Fourier transformation. We demonstrated the ability of B3-SRT for high contrast deep tissue Raman measurements in a highly scattering two-layer tissue phantom (e.g., fat–bone tissue model). With the use of a nondiffracting Bessel beam in turbid tissue, the B3-SRT technique provides an approximately ∼3.7-fold improvement in deep tissue Raman detection compared to confocal Raman spectroscopy. Further, with the benefit of the effective suppression of the randomly scattered photon interference enabled by the Bessel beam dynamic beating excitation and the Bessel-shaped collection, B3-SRT gives ∼1.5-fold enhancement in deep tissue Raman spectral contrast in comparison with confocal Raman spectroscopy. We anticipate that the B3-SRT technique developed has the potential to facilitate high contrast depth-resolved deep tissue Raman measurements in biomedical systems

Visualization 1: Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging

SRS video imaging on 1.0 µm PMMA beads acquired during Brownian motion in water. Originally published in Optics Letters on 01 September 2016 (ol-41-17-3960

Large size rearrangement identified in CTEPH patients and PE without PH patients.

<p>Large size rearrangement identified in CTEPH patients and PE without PH patients.</p

Visualization 2: Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy

3-D colocalized multimodal nonlinear microscopic images of dental caries Originally published in Biomedical Optics Express on 01 April 2016 (boe-7-4-1284

The primer sequences used for PCR amplification of coding sequences of 7 pulmonary arterial hypertension-associated genes.

<p>The primer sequences used for PCR amplification of coding sequences of 7 pulmonary arterial hypertension-associated genes.</p

categories of gene mutations in CTEPH patients and PE without PH patients.

<p>categories of gene mutations in CTEPH patients and PE without PH patients.</p

Visualization 1: Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy

3-D colocalized multimodal nonlinear microscopic images of DEJ in the tooth Originally published in Biomedical Optics Express on 01 April 2016 (boe-7-4-1284

Gene mutations in Chinese CTEPH patients and PE without PH patients.

<p>Gene mutations in Chinese CTEPH patients and PE without PH patients.</p

Clinical characteristics of study subjects.

<p>Clinical characteristics of study subjects.</p