DataSheet_2_A novel signature of aging-related genes associated with lymphatic metastasis for survival prediction in patients with bladder cancer.xlsx

BackgroundThe predominant and most prevalent form of metastatic bladder cancer (BCa) is lymphatic metastasis, which is associated with a highly dismal prognosis for patients. Aging-related genes (ARGs) are believed to contribute significantly to tumor development. However, the effect of ARGs on lymphatic metastasis of BCa is unclear. This research sought to establish a prognosis model based on ARGs associated with lymphatic metastasis in BCa.MethodsWe downloaded BCa data from the TCGA and GEO databases and ARGs from the Aging Atlas database. The least absolute shrinkage and selection operator (LASSO) approach was applied to obtain the characteristic ARGs of risk signature in the TCGA cohort. Verification was done using the GSE13507 dataset. The R package ‘ConsensusClusterPlus’ was employed to identify the molecular subtypes based on the characteristic ARGs. Protein-Protein interaction network, MCODE analysis, enrichment analysis (KEGG, GO, GSEA), and immune infiltration analysis were performed to investigate underlying mechanisms. EdU, migration and invasion assays, wound healing assays, immunofluorescence staining, and quantitative polymerase chain reaction were conducted to evaluate the impact of ELN on the proliferative, migratory, and invasive capacities of BCa cells.ResultsWe identified 20 differently expressed ARGs. A four ARGs risk signature (EFEMP1, UCHL1, TP63, ELN) was constructed in the TCGA cohort. The high-risk group (category) recorded a reduced overall survival (OS) rate relative to the low-risk category (hazard ratio, 2.15; P ConclusionWe constructed a four-ARG risk signature and identified two aging molecular subtypes. This signature could serve as an effective survival predictor for patients with BCa.</p

DataSheet_3_A novel signature of aging-related genes associated with lymphatic metastasis for survival prediction in patients with bladder cancer.xls

BackgroundThe predominant and most prevalent form of metastatic bladder cancer (BCa) is lymphatic metastasis, which is associated with a highly dismal prognosis for patients. Aging-related genes (ARGs) are believed to contribute significantly to tumor development. However, the effect of ARGs on lymphatic metastasis of BCa is unclear. This research sought to establish a prognosis model based on ARGs associated with lymphatic metastasis in BCa.MethodsWe downloaded BCa data from the TCGA and GEO databases and ARGs from the Aging Atlas database. The least absolute shrinkage and selection operator (LASSO) approach was applied to obtain the characteristic ARGs of risk signature in the TCGA cohort. Verification was done using the GSE13507 dataset. The R package ‘ConsensusClusterPlus’ was employed to identify the molecular subtypes based on the characteristic ARGs. Protein-Protein interaction network, MCODE analysis, enrichment analysis (KEGG, GO, GSEA), and immune infiltration analysis were performed to investigate underlying mechanisms. EdU, migration and invasion assays, wound healing assays, immunofluorescence staining, and quantitative polymerase chain reaction were conducted to evaluate the impact of ELN on the proliferative, migratory, and invasive capacities of BCa cells.ResultsWe identified 20 differently expressed ARGs. A four ARGs risk signature (EFEMP1, UCHL1, TP63, ELN) was constructed in the TCGA cohort. The high-risk group (category) recorded a reduced overall survival (OS) rate relative to the low-risk category (hazard ratio, 2.15; P ConclusionWe constructed a four-ARG risk signature and identified two aging molecular subtypes. This signature could serve as an effective survival predictor for patients with BCa.</p

DataSheet_1_A novel signature of aging-related genes associated with lymphatic metastasis for survival prediction in patients with bladder cancer.xlsx

BackgroundThe predominant and most prevalent form of metastatic bladder cancer (BCa) is lymphatic metastasis, which is associated with a highly dismal prognosis for patients. Aging-related genes (ARGs) are believed to contribute significantly to tumor development. However, the effect of ARGs on lymphatic metastasis of BCa is unclear. This research sought to establish a prognosis model based on ARGs associated with lymphatic metastasis in BCa.MethodsWe downloaded BCa data from the TCGA and GEO databases and ARGs from the Aging Atlas database. The least absolute shrinkage and selection operator (LASSO) approach was applied to obtain the characteristic ARGs of risk signature in the TCGA cohort. Verification was done using the GSE13507 dataset. The R package ‘ConsensusClusterPlus’ was employed to identify the molecular subtypes based on the characteristic ARGs. Protein-Protein interaction network, MCODE analysis, enrichment analysis (KEGG, GO, GSEA), and immune infiltration analysis were performed to investigate underlying mechanisms. EdU, migration and invasion assays, wound healing assays, immunofluorescence staining, and quantitative polymerase chain reaction were conducted to evaluate the impact of ELN on the proliferative, migratory, and invasive capacities of BCa cells.ResultsWe identified 20 differently expressed ARGs. A four ARGs risk signature (EFEMP1, UCHL1, TP63, ELN) was constructed in the TCGA cohort. The high-risk group (category) recorded a reduced overall survival (OS) rate relative to the low-risk category (hazard ratio, 2.15; P ConclusionWe constructed a four-ARG risk signature and identified two aging molecular subtypes. This signature could serve as an effective survival predictor for patients with BCa.</p

Table_1_A novel signature of aging-related genes associated with lymphatic metastasis for survival prediction in patients with bladder cancer.docx

BackgroundThe predominant and most prevalent form of metastatic bladder cancer (BCa) is lymphatic metastasis, which is associated with a highly dismal prognosis for patients. Aging-related genes (ARGs) are believed to contribute significantly to tumor development. However, the effect of ARGs on lymphatic metastasis of BCa is unclear. This research sought to establish a prognosis model based on ARGs associated with lymphatic metastasis in BCa.MethodsWe downloaded BCa data from the TCGA and GEO databases and ARGs from the Aging Atlas database. The least absolute shrinkage and selection operator (LASSO) approach was applied to obtain the characteristic ARGs of risk signature in the TCGA cohort. Verification was done using the GSE13507 dataset. The R package ‘ConsensusClusterPlus’ was employed to identify the molecular subtypes based on the characteristic ARGs. Protein-Protein interaction network, MCODE analysis, enrichment analysis (KEGG, GO, GSEA), and immune infiltration analysis were performed to investigate underlying mechanisms. EdU, migration and invasion assays, wound healing assays, immunofluorescence staining, and quantitative polymerase chain reaction were conducted to evaluate the impact of ELN on the proliferative, migratory, and invasive capacities of BCa cells.ResultsWe identified 20 differently expressed ARGs. A four ARGs risk signature (EFEMP1, UCHL1, TP63, ELN) was constructed in the TCGA cohort. The high-risk group (category) recorded a reduced overall survival (OS) rate relative to the low-risk category (hazard ratio, 2.15; P ConclusionWe constructed a four-ARG risk signature and identified two aging molecular subtypes. This signature could serve as an effective survival predictor for patients with BCa.</p

Postsynthesis and Effective Baeyer–Villiger Oxidation Properties of Hierarchical FAU-type Stannosilicate

Sn–Y zeolite, with hierarchical pore systems and extremely low Al content, was successfully prepared via a convenient postsynthetic route which involves proper predealumination and subsequent (NH₄)₂SnCl₆ treatment under mild aqueous condition. The Sn ions were incorporated into the framework of properly dealuminated Y zeolite through reacting with the defect sites generated in the industrial steaming treatment, the first-step acid treatment, and the second Sn incorporation process under acidic conditions. The acidic medium achieved by adding HCl in the Sn incorporation process affected not only the amount of incorporated Sn and residual Al but also the coordination state of inserted Sn ions. Compared with hydrothermally synthesized Sn-Beta, postsynthesized Sn–Y zeolite exhibited outstanding catalytic performances in the Baeyer–Villiger oxidation reactions of ketones especially when bulky <i>tert</i>-butyl hydroperoxide was employed as the oxidant due to an open pore structure of 3-dimensional 12-membered ring (12-MR) channels of FAU topology as well as the dealumination-derived intracrystal mesoporosity. Moreover, alkali metal ions modification was shown to be an effective approach for enhancing the selectivity of lactones

Odd−Even Effect in Free Radical Polymerization of Optically Active 2,5-Bis[(4‘-alkoxycarbonyl)- phenyl]styrene

Odd−Even Effect in Free Radical Polymerization of Optically Active 2,5-Bis[(4‘-alkoxycarbonyl)- phenyl]styren

Sensitivity analysis after each study was excluded by turns.

Sensitivity analysis after each study was excluded by turns.</p

Summary of studies of the comparison of the effect of pain relief of dorsal penile nerve block and lidocaine cream in circumcision.

Summary of studies of the comparison of the effect of pain relief of dorsal penile nerve block and lidocaine cream in circumcision.</p

Anthocyanin Accumulation and Molecular Analysis of Correlated Genes in Purple Kohlrabi (Brassica oleracea var. <i>gongylodes</i> L.)

Kohlrabi (Brassica oleracea var. <i>gongylodes</i> L.) is an important dietary vegetable cultivated and consumed widely for the round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation in the leaf and swollen stem. Here, different kinds of anthocyanins were separated and identified from the purple kohlrabi cultivar (Kolibri) by high-performance liquid chromatography–electrospray ionization tandem mass spectrometry. In order to study the molecular mechanism of anthocyanin biosynthesis in purple kohlrabi, the expression of anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi and a green cultivar (Winner) was examined by quantitative PCR. In comparison with the colorless parts in the two cultivars, most of the anthocyanin biosynthetic genes and two transcription factors were drastically upregulated in the purple tissues. To study the effects of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin contents and transcripts of associated genes were analyzed in sprouts of both cultivars grown under light and dark conditions

Risk of bias graph and risk of bias summary.

Risk of bias graph and risk of bias summary.</p