Trialkoxysilane-Induced Iridium-Catalyzed para-Selective C–H Bond Borylation of Arenes

The data includes condition screening, substrate data, synthetic applications, and theoretical calculations for the para C-H borylation of arenes

Two New Monascus Metabolites with Strong Blue Fluorescence Isolated from Red Yeast Rice

Red yeast rice obtained as cultures of Monascus AS3.4444 on rice was extracted and analyzed by high-performance liquid chromatography (HPLC). Two new Monascus metabolites with similar fluorescence spectra (λex = 396 nm, λem = 460 nm) and UV absorption spectra (λmax = 386 nm) were detected. They were isolated by rechromatography on a silica gel column and semipreparative HPLC, and two strong blue fluorescent compounds were obtained. Their structures were elucidated by electrospray ionization mass spectrometry (ESI−MS), electrospray ionization tandem mass spectrometry (ESI−MS/MS), intensive ESI−MS, and nuclear magnetic resonance spectroscopy (1H NMR, 13C NMR, COSY, and HMBC) studies. High-resolution mass spectrometry indicated the molecular formulas C21H24O5 and C23H28O5. The two new compounds, named monasfluore A and monasfluore B, respectively, contain a alkyl side chain, γ-lactone, and propenyl group, whereas the more lipophilic compound, monasfluore B, is a higher homologue of monasfluore A, with the more lipophilic octanoyl instead of the hexanoyl side chain

Additional file 1 of Effects of some flavonoids on the mycotoxin citrinin reduction by Monascus aurantiacus Li AS3.4384 during liquid-state fermentation

Additional file 1.Fig.S1. Total ion chromatogram of UPLC-QTOF-MS of the liquid fermentation samples following addition of different flavonoids and blank group. 2-citrinin, 7- monascin, 8- ankaflavin. Fig. S2 QTOF-MS of several main compounds in the liquid fermentation samples following addition of different flavonoids. Fig. S3 The liquid fermentation samples following addition of different flavonoids and blank group

Metabolic Effects of the <i>pksCT</i> Gene on Monascus aurantiacus Li As3.4384 Using Gas Chromatography–Time-of-Flight Mass Spectrometry-Based Metabolomics

Monascus spp. have been used for the production of natural pigments and bioactive compounds in China for several centuries. Monascus can also produce the mycotoxin citrinin, restricting its use. Disruption of the <i>pksCT</i> gene in Monascus aurantiacus Li AS3.4384 reduces citrinin production capacity of this strain (Monascus PHDS26) by over 98%. However, it is unclear how other metabolites of M. aurantiacus Li AS3.4384 (the wild-type strain) are affected by the <i>pksCT</i> gene. Here, we used metabolomic analyses to compare red yeast rice (RYR) metabolite profiles of the wild-type strain and Monascus PHDS26 at different stages of solid-state fermentation. A total of 18 metabolites forming components within the glycolysis, acetyl-CoA, amino acid, and tricarboxylic acid (TCA) cycle metabolic processes were found to be altered between the wild-type strain and Monascus PHDS26 at different stages of solid-state fermentation. Thus, these findings provide important insights into the metabolic pathways affected by the <i>pksCT</i> gene in M. aurantiacus

Exopolysaccharides from Genistein-Stimulated Monascus purpureus Ameliorate Cyclophosphamide-Induced Intestinal Injury via PI3K/AKT-MAPKs/NF-κB Pathways and Regulation of Gut Microbiota

Exopolysaccharides from genistein-stimulated Monascus purpureus (G-EMP) exhibited immunomodulatory potential in vitro, but whether it had immune-enhancing effects in vivo and its potential mechanism are not yet known. Here, the immunomodulatory effects of G-EMP were investigated by establishing an immunosuppressed mouse model treated with cyclophosphamide (Cy). The results suggested that G-EMP effectively alleviated the signs of weight reduction and diet reduction caused by Cy, increased fecal water content and splenic index, and decreased the oxidative stress of the liver. Simultaneously, G-EMP improved Cy-induced intestinal injury by restoring villus length, increasing the number of cupped cells, upregulating the expression of mucin and tight junction proteins, and downregulating the ratio of apoptotic proteins (Bax/Bcl-2). It also boosted the levels of mouse colonic cytokines, CD4+ and CD8+ T cells. Additionally, G-EMP markedly enhanced immunomodulation via the activation of PI3K/AKT-MAPKs/NF-κB signal pathways. Furthermore, G-EMP intervention displayed a positive association with most immunological indexes by elevating the levels of short-chain fatty acids, varying gut microbiota composition, and enhancing beneficial bacteria (Lactobacillaceae, Prevotellaceae, and S24-7). These findings demonstrated that G-EMP can strengthen immunity, repair intestinal mucosal damage, regulate gut microbiota, and be a potential source of prebiotics

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Inflammatory bowel disease is a major health problem that can lead to prolonged damage to the digestive system. This study investigated the effects of an exopolysaccharide from genistein-stimulated Monascus purpureus (G-EMP) in a mouse model of colitis to clarify its molecular mechanisms and identified its structures. G-EMP (Mw = 56.4 kDa) was primarily consisted of → 4)-α-D-Galp-(1 →, → 2,6)-α-D-Glcp-(1→ and →2)-β-D-Manp-(1 → , with one of the branches being α-D-Manp-(1 →. G-EMP intervention reduced the loss of body weight, degree of colonic damage and shortening, disease activity index scores, and histopathology scores, while restoring goblet cell production and oxidative homeostasis, repairing colonic functions, and regulating inflammatory cytokines. RNA sequencing and Western blot analysis indicated that G-EMP exerts anti-inflammatory properties by suppressing the TLR4/MAPK/NF-κB inflammatory signaling pathway. G-EMP modulated the gut microbiota by improving its diversities, elevating the relative abundances of beneficial bacteria, declining the Firmicutes/Bacteroidota value, and regulating the level of short-chain fatty acids (SCFAs). Correlation analysis demonstrated strong links between SCFAs, gut microbiota, and the inflammatory response, indicating the potential of G-EMP to prevent colitis