sj-docx-1-tag-10.1177_17562848221125308 – Supplemental material for Efficacy and safety comparison of Helicobacter pylori eradication between vonoprazan dual therapy versus triple therapy: a systematic review and meta-analysis

Supplemental material, sj-docx-1-tag-10.1177_17562848221125308 for Efficacy and safety comparison of Helicobacter pylori eradication between vonoprazan dual therapy versus triple therapy: a systematic review and meta-analysis by Zheyu Wang and Fen Wang in Therapeutic Advances in Gastroenterology</p

Multifunctional Surface Modification of Nanodiamonds Based on Dopamine Polymerization

Surface functionalization of nanodiamonds (NDs), which is of great interest in advanced material and therapeutic applications, requires the immobilization of functional species, such as nucleic acids, bioprobes, drugs, and metal nanoparticles, onto NDs’ surfaces to form stable nanoconjugates. However, it is still challenging to modify the surface of NDs due to the complexity of their surface chemistry and the low density of each functional group on the surfaces of NDs. In this work, we demonstrate a general applicable surface functionalization approach for the preparation of ND-based core–shell nanoconjugates using dopamine polymerization. By taking advantage of the universal adhesion and versatile reactivity of polydopamine, we have effectively conjugated DNA and silver nanoparticles onto NDs. Moreover, the catalytic activity of ND-supported silver nanoparticle was characterized by the reduction of 4-nitrophenol, and the addressability of NDs was tested through DNA hybridization that formed satellite ND–gold nanorod conjugation. This simple and robust method we have presented may significantly improve the capability for attaching various functionalities onto NDs and open up new platforms for applications of NDs

Sequence-Defined Peptidocopolymers: The Effect of Small Molecular Linkers

In this paper, the contribution of nonpeptido small molecular linkers to the properties of sequence-defined peptidocopolymers was investigated. We synthesized four novel bioinspired peptidocopolymers (<b>P1–P4</b>) based on elastin motif pentapeptide (Gly-Pro-Gly-Gly-Ala) by step growth polymerization. Small molecular linkers, including tetraethylene glycol (<b>M1</b>), adipic acid (<b>M2</b>), isophthalic acid (<b>M3</b>), and terephthalic acid (<b>M4</b>) with different length and flexibility are employed to tune the conformation, physical, and mechanical properties of the corresponding peptidocopolymers <b>P1</b>–<b>P4</b> respectively. Raman spectroscopy, solid state NMR, and circular dichroism spectroscopy were used to characterize the conformation of the four peptidocopolymers. The experimental results were further confirmed by molecular dynamics simulation of typical <b>P2</b> and <b>P4</b> with different repeating units. High ratio of β-turn conformation was observed in <b>P2</b> due to flexible linker <b>M2</b>; while affected by the hydrophobic and rigid <b>M4</b> linker, <b>P4</b> retained less β-turn conformation and showed drastic difference on macroscopic properties. These simple step growth synthesis techniques provide an efficient approach toward a broad range of bioinspired peptidocopolymers, which takes a further insight into the significant effect of nonpeptido linkages toward chemical-synthesized peptidocopolymers

Stratified analyses of study type for the association between MPV and psoriasis.

Stratified analyses of study type for the association between MPV and psoriasis.</p

Flowchart of literature screening in the meta-analysis.

Flowchart of literature screening in the meta-analysis.</p

Image_4_Construction and analysis of competing endogenous RNA network and patterns of immune infiltration in abdominal aortic aneurysm.TIF

BackgroundVarious studies have highlighted the role of circular RNAs (circRNAs) as critical molecular regulators in cardiovascular diseases, but its role in abdominal aortic aneurysm (AAA) is unclear. This study explores the potential molecular mechanisms of AAA based on the circRNA-microRNA (miRNA)-mRNA competing endogenous RNA (ceRNA) network and immune cell infiltration patterns.MethodsThe expression profiles of circRNAs (GSE144431) and mRNAs (GSE57691 and GSE47472) were obtained from the Gene Expression Omnibus (GEO). Then, the differentially expressed circRNAs (DEcircRNAs) and mRNAs (DEmRNAs) between AAA patients and healthy control samples, and the target miRNAs of these DEmRNAs and DEcircRNAs were identified. Based on the miRNA-DEmRNAs and miRNA-DEcircRNAs pairs, the ceRNA network was constructed. Furthermore, the proportion of the 22 immune cell types in AAA patients was assessed using cell type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm. The expressions of key genes and immune cell infiltration were validated using clinical specimens.ResultsA total of 214 DEmRNAs were identified in the GSE57691 and GSE47472 datasets, and 517 DEcircRNAs were identified in the GSE144431 dataset. The ceRNA network included 19 circRNAs, 36 mRNAs, and 68 miRNAs. Two key genes, PPARG and FOXO1, were identified among the hub genes of the established protein-protein interaction between mRNAs in the ceRNA network. Moreover, seven types of immune cells were differentially expressed between AAA patients and healthy control samples. Hub genes in ceRNA, such as FOXO1, HSPA8, and RAB5C, positively correlated with resting CD4 memory T cells or M1 macrophages, or both.ConclusionIn conclusion, a ceRNA interaction axis was constructed. The composition of infiltrating immune cells was analyzed in the abdominal aorta of AAA patients and healthy control samples. This may help identify potential therapeutic targets for AAA.</p

Forest plot of MPV values and the presence of psoriasis.

Forest plot of MPV values and the presence of psoriasis.</p

General characteristic of the included studies.

General characteristic of the included studies.</p

Image_1_Construction and analysis of competing endogenous RNA network and patterns of immune infiltration in abdominal aortic aneurysm.TIF

BackgroundVarious studies have highlighted the role of circular RNAs (circRNAs) as critical molecular regulators in cardiovascular diseases, but its role in abdominal aortic aneurysm (AAA) is unclear. This study explores the potential molecular mechanisms of AAA based on the circRNA-microRNA (miRNA)-mRNA competing endogenous RNA (ceRNA) network and immune cell infiltration patterns.MethodsThe expression profiles of circRNAs (GSE144431) and mRNAs (GSE57691 and GSE47472) were obtained from the Gene Expression Omnibus (GEO). Then, the differentially expressed circRNAs (DEcircRNAs) and mRNAs (DEmRNAs) between AAA patients and healthy control samples, and the target miRNAs of these DEmRNAs and DEcircRNAs were identified. Based on the miRNA-DEmRNAs and miRNA-DEcircRNAs pairs, the ceRNA network was constructed. Furthermore, the proportion of the 22 immune cell types in AAA patients was assessed using cell type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm. The expressions of key genes and immune cell infiltration were validated using clinical specimens.ResultsA total of 214 DEmRNAs were identified in the GSE57691 and GSE47472 datasets, and 517 DEcircRNAs were identified in the GSE144431 dataset. The ceRNA network included 19 circRNAs, 36 mRNAs, and 68 miRNAs. Two key genes, PPARG and FOXO1, were identified among the hub genes of the established protein-protein interaction between mRNAs in the ceRNA network. Moreover, seven types of immune cells were differentially expressed between AAA patients and healthy control samples. Hub genes in ceRNA, such as FOXO1, HSPA8, and RAB5C, positively correlated with resting CD4 memory T cells or M1 macrophages, or both.ConclusionIn conclusion, a ceRNA interaction axis was constructed. The composition of infiltrating immune cells was analyzed in the abdominal aorta of AAA patients and healthy control samples. This may help identify potential therapeutic targets for AAA.</p

Subgroup analysis of MPV in psoriasis.

Subgroup analysis of MPV in psoriasis.</p