51 research outputs found

    Fig 3 -

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    Relative expression levels of target genes in M. persicae after starvation normalized to Actin (A) and 18S (B) alone and in combination (C). Values are shown as the mean ± SEM. Bars with different letters are significantly different from each other (P < 0.05). Lowercase letters indicate the comparison among different starvation time for one target gene normalized with the same housekeeping gene. Uppercase letters represent the comparison among different normalized genes for one target gene under the same starvation time.</p

    Single control normalization error <i>E</i> for four target genes under different biotic and abiotic conditions in <i>M</i>. <i>persicae</i>.

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    The boxes indicate the 25th and 75th percentiles. Lines across the boxes represent the medians, and upper and lower dots represent the maximum and minimum values, respectively.</p

    Fig 4 -

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    Expression of target genes at different developmental stages of M. persicae normalized to Actin (A) and 18S (B) alone and in combination (C). Values are shown as the mean ± SEM. Bars with different letters are significantly different from each other (P < 0.05). Lowercase letters indicate the comparison among different developmental stages for one target gene normalized with the same housekeeping gene. Uppercase letters represent the comparison among different normalized genes for one target gene under the same developmental stage.</p

    EBNA1 sequence variations in EBVaGC.

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    <p>Numbers across the top correspond to the amino acid positions under which the B95-8 prototype amino acid and nucleotide sequence is listed. Different patterns are noted to the left column, while the specimens showing identical sequences to each other are listed by a representative isolate in the second column. The followed numbers in the parentheses denote the amount of the identical sequences from EBVaGC. Four EBVaGC specimen from GRC were not amplified successfully. V-val was the only variant in all EBVaGC cases. The initial specimen identified as EBVaGC was also being analysed.</p

    EBER sequence variations in 12 EBVaGC in GRC and 3EBVaGC in RGC.

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    <p>Names in the left column refer to EBER variants identified in this study. Names in the near column refer to the representative isolates, and the numbers in the parentheses after the name denote the number of isolates carried identical sequence with the representative isolate. An asterisk indicates a deletion of a nucleotide. EB-6m was the only variant in the present study. The initial specimen identified as EBVaGC was also being analysed.</p

    PCR analysis for EBV genotyping for 1/2,F/f,C/D.

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    <p><b>Lanes 1–8:</b> Lane M, DL 2000 DNA Marker; lane 1, negative control; lane 2, B95-8 prototype (Type 1); lane 5,7,8, representative cases of Type 1; lane 3,4,6,representative case of co-infection with Type 1 and 2; no subtype 2 was detected in the study. <b>Lanes 9–16:RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI W1/I1 region.</b> Lane M, DL2000 DNA Marker; lane 9, negative control; lane 10, Raji prototype (Type D); lane 11, 13 and 15, representative cases of Type C strains; lane 12,14,16, representative cases of Type D strains. <b>Lanes 17–24: RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI F region.</b> Lane M, DL2000 DNA Marker; lane 17, negative control; lane 18, B95-8 prototype (Type F); lane 19–24 representative cases of the Type F strains; No type f strain was found in the present study.</p
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