Cumulative survival of patients stratified by the number of lymph node metastases (pN).

<p>Cumulative survival of patients stratified by the number of lymph node metastases (pN).</p

Comparison of receiver operating characteristic curves based on the metastatic lymph node ratio (MLR) and the number of positive lymph nodes (pN).

<p>Comparison of receiver operating characteristic curves based on the metastatic lymph node ratio (MLR) and the number of positive lymph nodes (pN).</p

Cumulative survival of patients stratified by metastatic lymph node ratio (MLR).

<p>Cumulative survival of patients stratified by metastatic lymph node ratio (MLR).</p

Data_Sheet_1_Surveillance for rhabdomyolysis after the consumption of crayfish in Wuhan, China, 2016–2022.PDF

ObjectivesTo analyze the epidemiological characteristics and etiology of crayfish-related rhabdomyolysis.MethodsCases of crayfish-related rhabdomyolysis in Wuhan were monitored, and professional training of city’s surveillance personnel was conducted. Unified questionnaires were used to collect data.ResultsThe first case of crayfish-related rhabdomyolysis occurred on July 12, 2016. Subsequently, 423 patients were reported over the next 7 years, with muscle pain, weakness, and chest distress as main symptoms. In total, 64.54% (273/423) of patients were females, and young adults (aged 20–49 years) account for 86.22% (363/423) of patients. The primary clinical presentations were muscle pain, muscle weakness, and chest discomfort. The median incubation time was 6 h. And the number of cases may be related to water levels in Yangzi river. Laboratory tests revealed elevated creatine kinase and myoglobin levels. In total, 95.16% (236/248) of patients had consumed crayfish tail shrimp and 91.53% (227/248) had consumed crayfish liver and pancreas (Female crayfish also contain ovaries). Only 25.00% (62/248) of patients had a history of alcohol consumption. On average, 227 patients consumed 15 (3–50) crayfish, of whom 84.14% (191/227) consumed more than 10 crayfish. All patients had a favorable prognosis.ConclusionCrayfish-related rhabdomyolysis is a kind of a case or cluster of patients present with severe myalgia or weakness of unknown etiology and mechanism disease in Wuhan, China, 2016–2022. Excessive consumption of crayfish may be a risk factor for the disease. The relationship between the specific parts of crayfish consumed and the onset of the disease is unclear, suggesting further research is needed to identify the relevant risk factors for the disease.</p

Largely different carotenogenesis in two pummelo fruits with different flesh colors

<div><p>Carotenoids in citrus fruits have health benefits and make the fruits visually attractive. Red-fleshed ‘Chuhong’ (‘CH’) and pale green-fleshed ‘Feicui’ (‘FC’) pummelo (<i>Citrus maxima</i> (Burm) Merr.) fruits are interesting materials for studying the mechanisms of carotenoid accumulation. In this study, particularly high contents of linear carotenes were observed in the albedo tissue, segment membranes and juice sacs of ‘CH’. However, carotenoids, especially β-carotene and xanthophylls, accumulated more in the flavedo tissue of ‘FC’ than in that of ‘CH’. Additionally, the contents of other terpenoids such as limonin, nomilin and abscisic acid significantly differed in the juice sacs at 150 days postanthesis. A dramatic increase in carotenoid production was observed at 45 to 75 days postanthesis in the segment membranes and juice sacs of ‘CH’. Different expression levels of carotenogenesis genes, especially the ζ-carotene desaturase (<i>CmZDS</i>), β-carotenoid hydroxylase (<i>CmBCH</i>) and zeaxanthin epoxidase (<i>CmZEP</i>) genes, in combination are directly responsible for the largely different carotenoid profiles between these two pummelo fruits. The sequences of eleven genes involved in carotenoid synthesis were investigated; different alleles of seven of eleven genes might also explain the largely different carotenogenesis observed between ‘CH’ and ‘FC’. These results enhance our understanding of carotenogenesis in pummelo fruits.</p></div

NanoLC-ESI-MS/MS spectrum of native glycans.

<p>a) MS/MS spectra of <i>m/z</i> 2060 with chemical composition of GlcNAc₄Man₃Gal₂NeuAcLac₁; b) MS/MS spectra of <i>m/z</i> 2078 with chemical composition of GlcNAc₄Man₃Gal₂NeuAc₁.</p

Typical NP-HPLC spectrum of 2-AA labeled glycans from the Fab and Fc fragment of the biosimilar of cetuximab.

<p>a) N-glycans on the Fab fragment; b) N-glycans on the Fc fragment. The compositions and structural schemes of glycans in each chromatographic peak are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170013#pone.0170013.s003" target="_blank">S2 Table</a> of the Electronic Supplementary Material.</p

Comprehensive N-Glycan Profiling of Cetuximab Biosimilar Candidate by NP-HPLC and MALDI-MS - Fig 1

<p>a) A representative schematic structure of monoclonal antibody and <i>N</i>-glycosylation sites on it. The main glycan moieties of the Fab and Fc fragment were shown in the frame. Structures and the monosaccharides are depicted following the CFG notation; b) flowchart of our method in this study.</p

MALDI-TOF MS spectrum of N-glycans enzymatically released from the biosimilar of cetuximab and cetuximab.

<p>a) native N-glycans before mild alkali treatment (pH 10 ammonium hydroxide); b) native N-glycans of the biosimilar after mild alkali treatment; c) native N-glycans from the cetuximab. The cartoons of possible structures of glycans were adapted from Glycoworkbench and structure is depicted following the CFG notation.</p

Typical NP-HPLC spectrum of 2-AA labeled glycans from the biosimilar of cetuximab.

<p>a) 2-AA labeled mAbs glycans before mild alkali treatment; b) 2-AA labeled mAbs glycans after mild alkali treatment.</p