Additional file 1 of Identifying the factors affecting ‘patient engagement’ in exercise rehabilitation

Additional file 1. Lay-person summary

Enhanced Stability Differentiation of Therapeutic Polyclonal Antibodies with All Ion Unfolding-Ion Mobility-Mass Spectrometry

Compared with monoclonal antibodies, polyclonal antibodies (pAbs) have rather significant characteristics, including lower cost, shorter production cycle, and higher affinity. Therefore, to facilitate their applications in clinic, it is equally critical to comprehensively characterize the conformational stabilities of pAb at the molecular weight-resolved scale, which is technically challenging due to the lack of an effective analytical tool capable of simultaneously providing both stability and molecular weight information within an acceptable error range. Ion mobility-mass spectrometry (IM-MS) has grown as an alternative to rapidly assess protein conformational stability with accurate molecular weight information maintained, especially when equipped with a collision-induced unfolding (CIU) regime. Dynamic and transient conformational intermediates can be captured with the CIU-IM-MS technique, adding to traditional static structural measurements with collisional cross section. Most CIU-IM-MS-centered protocols are focusing on the application to isolated, targeted protein ions, namely, analyzing one single charge state at one time, limiting its analytical throughput and speed. In this study, we employed an enhanced unfolding regime, all ion unfolding (AIU), capable of the simultaneous operation of numerous ions at a time during stepped unfolding processes to analyze pAb. Results show that AIU can quantitatively characterize the subtle differences in conformational stability among four structurally similar pAbs with improved resolving capability by around a 2–4-fold increment in both stability and structure differentiating parameters. Besides, AIU also benefits from considerably saved time cost and improved spectrum quality with an elevated signal-to-noise ratio

Funnel plot for the random-effects between-group meta-analysis on blood glutamate levels in persons with ASD and controls.

<p>Funnel plot for the random-effects between-group meta-analysis on blood glutamate levels in persons with ASD and controls.</p

Forest plot for the random-effects between-group meta-analysis of blood glutamate levels in persons with ASD and controls.

<p>Forest plot for the random-effects between-group meta-analysis of blood glutamate levels in persons with ASD and controls.</p

Characteristics of the twelve studies included in the meta-analysis.

<p>Characteristics of the twelve studies included in the meta-analysis.</p

Meta-regression of blood glutamate levels in persons with ASD and controls.

<p>Meta-regression of blood glutamate levels in persons with ASD and controls.</p

PRISMA checklist.

(DOC)</p

Plasma-Droplet Fusion-Mass Spectrometry Reveals Sub-Millisecond Protein Unfolding Dynamics Induced by Reactive Oxygen Species

Investigating the connection between reactive oxygen species (ROS) and oxidative protein unfolding is critical to reveal the mechanisms underlying disease involving elevated ROS and protein misfolding. This could inform the development of therapeutics targeting cells based on their redox status. In this study, we developed a plasma-droplet fusion-mass spectrometry platform to rapidly assess protein resilience to ROS. This home-built system fuses ROS generated from the microplasma source with protein microdroplets from a tunable nanospray source. At the droplet-plasma intersection, ROS interact with proteins before entering the mass spectrometer for mass identification and structural characterization. Benefiting from the small-sized microdroplet with adjustable traveling velocity, the platform enables the first sub-millisecond kinetic study of ROS-induced protein unfolding, with a rate constant of approximately 1.81 ms–1. Capturing ROS-induced protein unfolding intermediates and the resultant ligand release dynamics can be extended to many more protein systems. We foresee broad applications for establishing previously undetected protein unfolding events when biologically impactful ROS are enriched in time and space with functional proteins and complexes

Summary of the results of blood glutamate levels in persons with ASD and controls.

<p>Summary of the results of blood glutamate levels in persons with ASD and controls.</p

In Vitro and in Vivo Characterization of a Foam-Like Polyurethane Bone Adhesive for Promoting Bone Tissue Growth

Biocompatible bone adhesive is a kind of promising biomaterial in orthopedic and trauma surgery. However, despite the urgent need, there is no appropriate system that can meet all the requirements for bone adhesive. In this study, a porous polyurethane adhesive (PUA) was fabricated, and the effects of water, polyisocyanate, and β-tricalcium phosphate (β-TCP) on the physicochemical and mechanical properties of PUAs were investigated. By varying the water content, PUAs with different surface morphologies and porosities were prepared. The adhesion strength and mechanical property of the adhesives could be enhanced by the addition of polyisocyanate and β-TCP. The adhesion strength of the PUA to bone was 2 times higher than that of clinical poly­(methyl methacrylate) bone cement. The in vitro cell culture and attachment assays indicated good biocompatibility of the PUAs. In vivo analysis in a rabbit model demonstrated that the porous structure of the PUA could facilitate the growth of cells and bone tissues