One-Pot Synthesis of Water-Soluble Glycosyl Azobenzenes and Their Photoswitching Properties in Water

Molecular photoswitches capable of reversible photoswitching in aqueous media are highly demanded for various biological applications and photopharmacology. Carbohydrates, as natural and abundant raw materials, provide opportunity to make photoswitches water-soluble through linking sugar to the photoswitching molecules. We have developed a one-pot synthesis method to prepare water-soluble glycosyl azobenzenes through DMC (2-chloro-1,3-dimethylimidazolinium chloride)-mediated glycosylation between sugar and dihydroxyazobenzenes (DHABs) in aqueous media. The scope of the method has been investigated with different mono- and disaccharides, as well as with p,p′- and o,o′-DHAB, with excellent 1,2-trans stereoselectivity. Diglycosylation products can also be obtained with an excess amount of monosaccharides in one step. We have also demonstrated the possibility of further functionalization on the azobenzene moiety of glycosyl azobenzene. Both mono- and diglycosyl azobenzenes showed excellent photoswitching properties in water with high fatigue resistance and good thermostability for the Z-isomers. Excellent E → Z photoisomerization of both mono- and diglycosylated azobenzenes (Z/E = 99/1) is observed under illumination at 365 nm, while back Z → E photoisomerization can be achieved with blue light (with E/Z = 80/20 at PSS485 for the diglycosyl derivative)

Table_1_Transition patterns of weight status: A cohort study of Chinese school-age children.docx

BackgroundChildhood overweight and obesity are increasing public concerns. However, little is known about the transition patterns of childhood weight status, especially in developing countries. In this study, we aimed to evaluate patterns of change in weight status and the risk factors among Chinese school-age children.MethodsThis retrospective cohort study included 2,334 children aged 6 years with complete 5-year (2012–2017) physical examination data in Minhang District, Shanghai. A time-homogeneous three-state Markov model was fit to the longitudinal data with dynamic outcomes (normal weight, overweight, and obesity).ResultsAccording to the Markov model, 42.3% of school-age children who were initially overweight transitioned to another weight status within 1 year, with 24.8% (95% confidence interval [CI]: 23.1, 27.0) transitioning to normal weight and 17.5% (95% CI: 15.9, 19.3) becoming obese. In contrast, children who were initially normal weight (92.9% [95% CI: 92.3, 93.5]) or obese (83.1% [95% CI: 81.1, 84.8]) tended to maintain their initial weight status. Male sex, semi-urban area, absence of late adiposity rebound, lower annual height increments, higher annual weight increments, and higher initial body mass index were significantly associated with a higher risk of developing or maintaining overweight and obesity (p ConclusionsThe weight status of Chinese school-age children is more likely to change among those who are initially overweight than in those who are initially obese. Interventions to promote healthy weight status may be more effective if key groups are targeted, such as overweight and pre-school-age children.</p

Reconstitution and Optimization of the Marmesin Biosynthetic Pathway in Yeast

Marmesin is essential in plant defense systems and exhibits various biological activities. In this study, we reconstituted the marmesin biosynthetic pathway in the Saccharomyces cerevisiae BY4741 chassis. We engineered the aromatic amino acid (AAA) biosynthetic pathways by introducing Escherichia coli-derived ppsA to improve the availability of the AAA precursor phosphoenolpyruvate, overexpressing the feedback inhibition resistance genes ARO4K229L and ARO7G141S to direct the metabolic flux toward the tyrosine branch, and deleting ARO10, PDC5, and PDC6 to reduce the byproducts from the Ehrlich pathway. The umbelliferone 6-dimethylallyltransferase (U6DT) and marmesin synthase (MS) involved in marmesin synthesis were optimized to increase marmesin production. Marmesin production was improved by truncating the transmembrane domains of PcU6DT, FcMS, and AtCPR1 and increasing the copy numbers of the genes encoding the truncated enzymes. Finally, a marmesin titer of 27.7 mg/L was obtained in shake flasks using the engineered yeast strain MU5. The constructed marmesin-producing strain provides the foundation for the green and large-scale production of pharmaceutically important furanocoumarins

Additional file 4 of Perceived extrinsic barriers hinder community detection and management of mild cognitive impairment: a cross-sectional study of general practitioners in Shanghai, China

Additional file 4. Path coefficients and hypothesis testing

Additional file 1 of Perceived extrinsic barriers hinder community detection and management of mild cognitive impairment: a cross-sectional study of general practitioners in Shanghai, China

Additional file 1: Table S1. Reliability and validity of Reflective Measurement Models

Additional file 3 of Perceived extrinsic barriers hinder community detection and management of mild cognitive impairment: a cross-sectional study of general practitioners in Shanghai, China

Additional file 3: Table S3. Indirect effects of knowledge on intended practice

Additional file 2 of Perceived extrinsic barriers hinder community detection and management of mild cognitive impairment: a cross-sectional study of general practitioners in Shanghai, China

Additional file 2: Table S2. Discriminant validity (HTMT) of the scales measuring perceived extrinsic barriers

Source reconstruction analyses findings.

Source reconstruction analyses findings.</p

Additional file 2 of Knowledge, attitudes, and practice of general practitioners toward community detection and management of mild cognitive impairment: a cross-sectional study in Shanghai, China

Additional file 2: Appendix File 2. Survey on the detection and management of mild cognitive impairment in community health services

Additional file 1 of Knowledge, attitudes, and practice of general practitioners toward community detection and management of mild cognitive impairment: a cross-sectional study in Shanghai, China

Additional file 1: Appendix File 1. The variance inflation factor (VIF) values for the Multicollinearity analyses