Gene ontology (GO) annotations of the annotated unigenes.

<p>12,503 unigenes were assigned to three GO categories containing 52 functional subcategories.</p

Venn diagram showing the comparison among <i>P. olivaceus</i> transcriptomic sequences with the known sequences from <i>D. rerio</i> and <i>P. olivaceus</i> EST deposited in the NCBI database.

<p>Venn diagram showing the comparison among <i>P. olivaceus</i> transcriptomic sequences with the known sequences from <i>D. rerio</i> and <i>P. olivaceus</i> EST deposited in the NCBI database.</p

Cluster of Orthologous Groups (COG) annotations of annotated unigenes.

<p>19,547 unigenes were classified into 26 COG categories.</p

Length distribution of the assembled (blue) and the annotated unigenes (red) of <i>P. olivaceus</i>.

<p>Length distribution of the assembled (blue) and the annotated unigenes (red) of <i>P. olivaceus</i>.</p

Distribution of top-hit species.

<p>Distribution of top-hit species.</p

Summary statistics of <i>P. olivaceus</i> transcriptome sequencing and assembly.

<p>Summary statistics of <i>P. olivaceus</i> transcriptome sequencing and assembly.</p

Immune pathways annotated in the <i>P.olivaceus</i> spleen transcriptome.

<p>Immune pathways annotated in the <i>P.olivaceus</i> spleen transcriptome.</p

Quantitative Real-time PCR (qRT—PCR) validation of the expressed genes in transcriptome sequencing.

<p>IRAK4: interleukin-1 receptor-associated kinase 4; IRF7: interferon regulatory factor 7; INFAR: interferon receptor 1; NFKB1: nuclear factor NF-kappa-B p105 subunit; TRAF6: TNF receptor-associated factor 6; AKT: RAC serine/threonine-protein kinase; IRAK1: interleukin-1 receptor-associated kinase 1; F2: coagulation factorII; TNFA: tumor necrosis factor superfamily. Values are presented as means ± standard deviation（n = 5）and the error bars indicate the standard deviation.</p