Additional file 1 of Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm

Additional file 1

Convenient and efficient method for synthesis of 2,4,6-triarylpyridines using catalytic amount of PEG₁₀₀₀-based dicationic acidic ionic liquid under solvent-free conditions

A convenient and efficient method for synthesis of substituted 2,4,6-triarylpyridines by the reaction of chalcones and ammonium acetate or via one-pot, three-component condensation of aromatic aldehydes with acetophenones and ammonium acetate has been developed by using the efficient catalyst PEG1000-based dicationic acidic ionic liquid (PEG1000-DAIL) under solvent-free conditions. Recycling studies have shown that the PEG1000-DAIL can be readily recovered and reused several times without significant loss of activity.</p

DataSheet1_Effects of Relative Molecular Weight Distribution and Isoelectric Point on the Swelling Behavior of Gelatin Films.docx

The swelling behavior of gelatin films with different extraction processes are investigated. The results showed that the swelling ratio of the gelatin film extracted by alkaline hydrolysis of collagen (type-B) in a range of pH environments was higher than the one extracted by enzymatic hydrolysis collagen (type-E). In the drug releasing simulation, type-B gelatin capsules also showed a faster collapse rate than type-E gelatin capsules. Based on analyzing relative molecular weight distribution of type-B and type-E gelatins, the more widely distributed relative molecular weight is the key attribution for enabling easier diffusion of water molecules inside the porous channels of peptide chains. Furthermore, with the pH of solution environment far from the isoelectric point (pI) of gelatin films, the swelling ratios were found to increase remarkably, which is due to electrostatic repulsion expanding the pore size of peptide chains. Finally, the addition of SO42− in gelatin film was performed to confirm the dominant effect of component compared to pI on swelling behavior of gelatin films.</p

Direct Atomic-Scale Imaging about the Mechanisms of Ultralarge Bent Straining in Si Nanowires

To safely and reliably use nanowires (NWs) for exploring new functions for different nanodevices, the mechanical properties and structural evolution of the nanowires under external stress become highly important. Large strain (up to 14%) bending experiments of Si NWs were conducted in a high-resolution transmission electron microscope at atomic resolution. The direct dynamic atomic-scale observations revealed that partial and full dislocation nucleation, motion, escape, and interaction were responsible for absorbing the ultralarge strain of up to 14% in bent Si nanowires. The prevalent full dislocation movement and interactions induced the formation of Lomer lock dislocations in the Si NWs. Finally, in contrast to the unlock process of Lomer dislocations that can happen in metallic materials, we revealed that the continuous straining on the Lomer dislocations induced a crystal–amorphous (c-a) transition in Si NWs. Our results provide direct explanation about the ultralarge straining ability of Si at the nanometer scale

Image_1_SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.TIF

Introduction: Aberrant activation of Semaphorin3C(SEMA3C) is widespread in human cancers. We aimed to analyze SEMA3C expression in cervical cancer and investigate the role of SEMA3C in cervical cancer and its underlying mechanism, which is important for exploring new therapeutic targets and prognostic factors.Materials and Methods: The expression of SEMA3C was examined in paraffin-embedded cervical cancer specimens. In vivo and in vitro assays were performed to validate the effect of SEMA3C on cervical cancer cell proliferation and p-ERK pathway activation. Gene Set Enrichment Analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data set.Results: SEMA3C expression was associated with poor survival in both the TCGA cohort and our cohort. Silencing of SEMA3C suppressed cervical cancer cell proliferation, colony formation ability, and the activation of the p-ERK signaling pathway in vitro. SEMA3C depletion inhibited tumor growth in vitro. GSEA also showed that the epithelial mesenchymal transition (EMT), TGFβ signaling pathway, angiogenesis, and extracellular matrix (ECM) receptor interactions are associated with a high SEMA3C expression phenotype.Conclusion: SEMA3C is correlated with poor prognosis of cervical cancer patients and promotes tumor growth via the activation of the p-ERK pathway.</p

Direct Atomic-Scale Imaging about the Mechanisms of Ultralarge Bent Straining in Si Nanowires

To safely and reliably use nanowires (NWs) for exploring new functions for different nanodevices, the mechanical properties and structural evolution of the nanowires under external stress become highly important. Large strain (up to 14%) bending experiments of Si NWs were conducted in a high-resolution transmission electron microscope at atomic resolution. The direct dynamic atomic-scale observations revealed that partial and full dislocation nucleation, motion, escape, and interaction were responsible for absorbing the ultralarge strain of up to 14% in bent Si nanowires. The prevalent full dislocation movement and interactions induced the formation of Lomer lock dislocations in the Si NWs. Finally, in contrast to the unlock process of Lomer dislocations that can happen in metallic materials, we revealed that the continuous straining on the Lomer dislocations induced a crystal–amorphous (c-a) transition in Si NWs. Our results provide direct explanation about the ultralarge straining ability of Si at the nanometer scale

Experimental Investigation of CO₂ Capture Capacity: Exploring Mesoporous Silica SBA-15 Material Impregnated with Monoethanolamine and Diethanolamine

A series of efficient adsorbents were prepared by impregnating mesoporous silica SBA-15 with different amounts of mono­ethanol­amine (MEA) and diethanol­amine (DEA) in order to improve CO2 capture capacity. The textural properties of pure and modified mesoporous SBA-15 materials were characterized by X-ray diffraction characterization, transmission electron microscopy, N2 adsorption–desorption test, and thermogravimetric analysis. When the ratio of DEA to SBA-15 is below 2, these molecules loaded on the support are relatively far away from saturating and allow the accessibility of CO2 molecules to the inner adsorption sites. Further increasing of the amine loading would reduce opportunities of CO2 to contact with internal amino sites, because the amine was covered in a multilayer form or even caking form on the SBA-15 pore surface. The similar performance was observed for MEA. Therefore, the adsorption capacity of CO2 increases with the amount of DEA or MEA content, but when the amount of MEA or DEA loaded on the mesoporous SBA-15 is further increased, the CO2 capture is influenced by the packing effect on the mesoporous hexagonal silica. Temperature effect on adsorption was also studied in the range of 30–90 °C, showing that, with the increase of temperature, the adsorptive amounts of adsorbents lessened gradually from the highest values at 30 °C since the thermodynamically controlled process. The mesoporous SBA-15 material impregnated with MEA or DEA can provide a perspective to further explore effective adsorbents for CO2 capture

Additional file 1: Table S1. of BmncRNAdb: a comprehensive database of non-coding RNAs in the silkworm, Bombyx mori

The detail information of RNA-seq datasets. All the samples used to the identification of the silkworm lncRNAs. (DOC 44 kb

Universal and High-Speed Zeptomolar Protein Serum Assay with Unprecedented Sensitivity

The accurate detection of trace protein biomarkers is critical for disease diagnosis, healthcare, and pathology research. Currently, the main predicaments of techniques are low sensitivity, prolonged procedures, and the need for specialized devices. Moreover, multistep handling and nonspecific biofouling can lead to high background noise and false positives. To overcome these barriers, a novel ultrasensitive electrochemical platform was developed by combining an electrochemistry approach with the silver mirror reaction to detect proteins at the zeptomolar level. This assay can be accomplished in about only 18 min. As a proof of the concept, human immunoglobulin G (h-IgG) as a model analyte exhibited an ultralow detection limit of 6.31 ag mL–1 (0.04 zeptomoles mL–1). This strategy can be exploited as a universal approach for the ultrasensitive detection of various proteins in clinical diagnostics and point-of-care testing

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR

<p>Introducing various mutations at multiple specific sites within a gene requires multiple steps of DNA manipulation, which is the initial, but limiting step of protein structure–function studies. In the present work, we standardized a simple and fast procedure to perform site-directed mutagenesis, multiple-site fragment deletion, insertion and substitution mutagenesis by a modified version of overlap extension polymerase chain reaction (PCR). In this procedure, target genes divided into several fragments based on the site of mutagenesis are amplified and annealed with their complementary overhanging, followed by extension and amplification to full-length gene with expected mutation(s) by PCR. Vectors inserted with the modified target gene are screened by colony PCR. By using the standardized procedure, we have easily generated single-site mutations, replaced/deleted DNA fragment into/from a target gene and engineered a cysteine-free protein. Practically, the standardized procedure provides an efficient choice for almost all kinds of mutagenesis, especially for multiple-site and large DNA fragment modification mutagenesis. Therefore, this method can be utilized to analyze protein structure and function, to optimize codons of genes for protein expression and to assemble genes of interest.</p