7 research outputs found

    Glomerular filtration and diuresis are related to the state of lipids peroxidation in female rats exposed to water-salt loads accompanied by chronic stress

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    Background. It is known about the existence of close functional link between adequate working kidney nephrons and bone metabolism. Focused monitoring of renal function is able to provide substantial assistance in the organization of preventive measures against latently emerging osteoporosis. Activation of lipoperoxidation is known to inhibit glomerular filtration, whereas antioxidants prevent this. We set ourselves the goal of clarifying correlations between glomerular filtration, as well as tubular water reabsorption and daily diuresis, on the one hand, and lipoperoxidation parameters, on the other hand. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 240-290 g, both intact and exposed to water-salt loads. By the size of the diuresis and the level of creatinine in plasma and urine, glomerular filtration and tubular reabsorption were calculated. The plasma levels of diene conjugates and malondyaldehide as well as catalase of plasma and superoxide dismutase of erythrocytes were determined. The levels of the first three parameters determined in urine too. Results. In conditions of water-salt loads accompanied by chronic stress, glomerular filtration is downregulated by malondialdehyde and superoxide dismutase; diuresis is also downregulated by malondialdehyde, but upregulated by catalase. Lipoperoxidation parameters determine kidney function by 52,8%. Conclusion. The obtained data indicate the role of lipoperoxidation as a accompaniment of chronic stress in the regulation of kidney functions

    Canonical analysis of neuroendocrine-metabolic and neuroendocrine-immune relationships at female rats

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    Background. It has been shown before that drinking mineral waters of different composition has a significant effect on the neuroendocrine, metabolic and immune parameters of healthy rats. We analyzed the canonical correlations between neuroendocrine parameters, on the one hand, and metabolic and immune parameters, on the other hand. Materials and Methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g. HRV parameters, major adaptation hormones, metabolism and immunity parameters were recorded. Results. The method of canonical correlation analysis revealed causal relationships between neuroendocrine and metabolic and neuroendocrine and immune parameters of the organism. The degree of neuroendocrine determination of individual sets of metabolic parameters ranges from 60% to 92%, and immune status from 87% to 96,5%. Conclusion. Effects of mineral waters on metabolism and immunity are realized through nervous and hormonal mechanisms

    General non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats

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    Background. This article begins with a series of articles on the effects on parameters of water-salt, nitrous and lipid metabolism, as well as the neuroendocrine-immune complex of mineral water, extracted from the bore located in the city Gertsa (Bukovyna, Ukraine). The chemical analysis prompted us to use waters Sophiya and Naftussya from spa Truskavets’ as a reference as well as an artificial salt analogue of Gertsa water, which contains no organic matter or trace elements. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats the parameters of metabolism and neuroendocrine-immune complex were registered. Results. Screening registered parameters found 42 among them who in rats subjected to water-salt loads, significantly different from that of intact rats, but on average the same group of animals that received liquids with different mineralization and chemical composition. Conclusion. Takes place nonspecific (general) reaction neuroendocrine-immune complex and metabolism in water-salt load as such, regardless of the specific chemical composition of fluids applied

    Role of organic carbon and nitrogen of mineral waters in their metabolic effects at female rats

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    Background. Organic substances, despite their presence in all drinking mineral waters, are still considered to be active only in the water of Naftussya type (which, due to mineralization less than 1 g/L, are not formally mineral), whereas the physiological activity of true mineral waters (which mineralization is greater than 1-2 g/L) are associated with electrolytes and trace elements. The purpose of this study was to clarify the role of organic carbon and nitrogen of mineral waters in their effects on certain parameters of metabolism. Material and Methods. Experiment was performed on 48 healthy female Wistar rats 240-290 g divided into 5 groups. Animals of the first group for 6 days administered a single tap water through the probe at a dose of 1,5 mL/100 g of body mass. In the second group we administered the water Naftussya from the Truskavets’ layer, in the third group the water Sophiya of the Truskavets’ field. The rats of the fourth group received the native water from the Gertsa (Bucovyna) field, and the last group its artificial salt analogue. The day after the completion of the drinking course in all rats some metabolic parameters were registered. Results. On the basis of the correlation analysis with step-by-step exclusion, four metabolites of blood as well as five metabolites of urine are included in the regressive model for organic carbon (R=0,697; R2=0,486; Adjusted R2=0,347; F(10,4)=3,5; p=0,0025). Organic nitrogen of mineral waters affects five metabolic parameters of urine only, but with approximately the same force (R=0,621; R2=0,385; Adjusted R2=0,312; F(5,4)=5,3; p=0,0008). Conclusion. Organic substances of mineral waters play an essential role in their effects on the parameters of metabolism

    Features of neuro-endocrine and immune reactions to various water-salt loads in female rats

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    Background. Previously, we have shown that the weekly load of rats with water-salt solutions of different chemical compositions causes both general and specific reactions of the parameters of metabolism. The purpose of this study is to identify under these conditions specific neuroendocrine and immune responses. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats some neuroendocrine and immune parameters were registered. Results. The method of discriminant analysis revealed 29 parameters of the neuroendocrine-immune complex (10 of them reflect the neuroendocrine regulation, 4 thymus mass and thymocytogram elements, 5 elements of splenocytogram, 10 elements of immunocytogram and leukocytogram of blood and parameters of phagocytosis), according to which the reaction on various water-salt loads are identified with an accuracy of 98.3%. Conclusion. The peculiarities of the reactions of the parameters of the neuroendocrine-immune complex are due to the content of water in sulfate, bicarbonate and magnesium, as well as organic carbon and nitrogen

    Mineral waters, metabolism, neuro-endocrine-immune complex, s. 252.

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    Dedicated to the 75th anniversary of Volodymyr Illich Kozyavkin with gratitude for the support of the Truskavetsian Scientific School of BalneologyThe monograph systematizes these writers and highlights the results of their own priority experimental and clinical-physiological studies of the impact of drinking mineral waters of Ukraine on neuroendocrine regulation, metabolism and immunity of healthy rats and patients in the process of rehabilitation of chronic pyelonephritis and cholecystitis in remission. In line with the concepts of functional-metabolic continuum and neuroendocrine-immune complex using the methods of factor, discriminant and canonical correlation analysis, it is demonstrated that mineral waters have both similar and specific physiologically favorable modulating effects on the parameters of the studied body systems. For specialists in medical rehabilitation, endocrinologists, immunologists, biochemists, pathophysiologists

    Features of metabolic reactions to various water-salt loads in female rats

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    Background. In the previous article we reported that screening registered parameters of water-salt, nitrous and lipid metabolism as well as the neuroendocrine-immune complex found 42 among them who in rats subjected to various water-salt loads, significantly different from that of intact rats, but on average the same group of animals that received liquids with different mineralization and chemical composition. The purpose of this article is to find out the features of the reactions of the parameters of metabolism. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats the parameters of water-salt, nitrous and lipid metabolism were registered. Results. Found that 16 metabolic parameters the maximum deviates from the level of intact rats under the influence of the salt analogue of Gertsa water, a smaller, but tangible effect is made by the Gertsa native water, even less effective waters Sofiya and Naftussya, instead of ordinary water is almost ineffective in relation to these metabolic parameters. The other 19 parameters deviates to a maximum extent from the reference level after the use of water Naftussya, fresh water is less effective, whereas quasi-isotonic liquids are practically inactive for these parameters. The remaining 13 parameters in animals that use normal water, deviates from intact control to the same extent as in the previous pattern, which, apparently, is also due to the stressful effects of the load course. Both Naftussya and Gertsa water and its salt analogue prevent the stress deviations of these parameters. Instead, by consumption of water Sofiya stresses deviations of these parameters is reversed. The method of discriminant analysis revealed 33 variables (among them 8 refer to plasma/erythrocytes electrolytes, 7 to electrolytes of urine, to other metabolic parameters of plasma 5 and urine 9, as well as glomerular filtration, canalicular reabsorption, diurese and urine osmolarity), the totality of which the metabolic reactions to various water-salt loads are identified (recognized) with an accuracy of 98,3%. Conclusion. The features of the reactions of the parameters of metabolism are due to the content in waters NaCl, SO42- as well as organic carbon and nitrogen
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