21 research outputs found
IL-22 attenuated airway hyperresponsiveness (AHR).
<p>(A, B) Invasive PFT of OVA stimulated IL-22 Tg(+) and Tg(−) mice was assessed (FlexiVent, SciQuest). Lung resistance at baseline and in response to increasing concentrations of methacholine (MCh) through inhalation was recorded and analyzed (*<i>P</i><0.05). The number of animals used in each group was as indicated. Data represented as Mean±SEM. OVA-induced IL-22 Tg(+) mice showed significantly lower lung resistance compared to OVA-induced IL-22 Tg(−) mice.</p
Serum immunoglobulin levels in OVA-induced allergic asthma in IL-22 Tg(+) (SPC-IL-22) and Tg(−) mice.
<p>Serum samples from IL-22 Tg(+) and Tg(−) mice were collected 48 hours after last OVA challenge. Immunoglobulins, including total and OVA-specific IgE, IgG1 and IgG2a were measured by ELISA and analyzed by one-way ANOVA. Data from individual animals were plotted. Both IL-22 Tg(+) and Tg(−) group showed much higher level either in total or in OVA-specific IgE, IgG1 and IgG2a than PBS treatment groups (<i>P</i><0.01). But there is no difference between IL-22 Tg(+) and Tg(−) groups (<i>P</i>>0.05).</p
Fast Photoinduced Large Deformation of Colloidal Spheres from a Novel 4‑arm Azobenzene Compound
A novel
4-arm shaped amphiphilic azobenzene compound was synthesized.
The tetraphenylethylene (TPE) core precursor was prepared and further
modified by azo coupling reaction at the four peripheral groups. Colloidal
spheres could be directly prepared by self-assembly of the prepared
amphiphilic azobenzene compound in selective solvents (THF/H<sub>2</sub>O), which were characterized by using transmission electron microscopy.
The colloid diameters could be controlled by adjusting the initial
compound concentration and water-adding rate in the preparation processes.
By irradiation with visible linearly polarized LED light (450 nm),
fast photoinduced deformation of the colloidal spheres along the polarization
direction was observed. A very large deformation degree (<i>l</i>/<i>d</i> > 4) could be easily obtained
Effect of IL-22 (CC10-IL-22) on cytokine and chemokine production in OVA-induced allergic asthma.
<p>Th1 cytokine, IFN-γ, and Th2 cytokines, IL-4 and IL-13, Th17 cytokine IL-17A, and chemokine eotaxin in the BAL were measured by ELISA. The number of animals in each group was indicated and data were shown as Mean±SEM. **<i>P</i><0.01 (unpaired Student t-test).</p
IL-22 alleviated OVA-induced eosinophilic inflammation in the lung.
<p>(A) High levels of IL-22 cytokine were seen in the BAL of PBS and OVA-stimulated IL-22 Tg(+) mice without difference between the two groups (<i>P</i>>0.05). When compared to Tg(−) mice, IL-22 concentrations in the BAL of Tg(+) mice were much higher than that in PBS and OVA-stimulated control groups (<i>P</i><0.0001). (B, C) BAL total cell and differentials counts showed that OVA-stimulated IL-22 Tg(+) group had a much higher percentage of eosinophils compared to OVA-stimulated Tg(−) mice (<i>P</i><0.0001), but there is no difference in the total cell counts (<i>P</i>>0.05). (D) Lung histology of OVA-induced allergic asthma in SPC-IL-22 Tg(+) mice and Tg(−) mice, H&E, IHC for MBP, and Alcian blue staining showed that OVA-induced IL-22 Tg(−) group had much more severe airway inflammation compared to OVA-induced IL-22 Tg(+) group.</p
Effect of IL-22 (SPC-IL-22) on OVA-induced systemic and local immune responses.
<p>Splenocytes and lymphocytes from peribronchial draining lymph nodes (DLN) from IL-22 Tg(+) and Tg(−) mice after OVA challenge were cultured and stimulated with medium control, OVA or CD3/CD28. Th1 cytokine, IFN-γ, and Th2 cytokine, IL-13 in the supernatant were measured by ELISA. The number of animals used in the experiments was indicated and data were shown as Mean±SEM. *<i>P</i><0.05 and **<i>P</i><0.01 (unpaired Student t-test).</p
DataSheet_1_The analysis of lysine succinylation modification reveals the mechanism of oxybenzone damaging of pakchoi (Brassica rapa L. ssp. chinensis).xlsx
Oxybenzone (OBZ), one of a broad spectrum of ultraviolet (UV) absorbents, has been proven to be harmful to both plants and animals, while omics analysis of big data at the molecular level is still lacking. Lysine succinylation (Ksuc) is an important posttranslational modification of proteins that plays a crucial role in regulating the metabolic network in organisms under stress. Here, we report the changes in intracellular Ksuc modification in plants under OBZ stress. A total of 1276 succinylated sites on 507 proteins were identified. Among these sites, 181 modified proteins were hypersulfinylated/succinylated in OBZ-stressed pakchoi leaves. Differentially succinylated proteins (DSPs) are distributed mainly in the chloroplast, cytoplasm, and mitochondria and are distributed mainly in primary metabolic pathways, such as reactive oxygen species (ROS) scavenging, stress resistance, energy generation and transfer, photosynthetic carbon fixation, glycolysis, and the tricarboxylic acid (TCA) cycle. Comprehensive analysis shows that Ksuc mainly changes the carbon flow distribution, enhances the activity of the antioxidant system, affects the biosynthesis of amino acids, and increases the modification of histones. The results of this study first showed the profiling of the Kusc map under OBZ treatment and proposed the adaptive mechanism of pakchoi in response to pollutants and other abiotic stresses at the posttranslational level, which revealed the importance of Ksuc in the regulation of various life activities and provides a reference dataset for future research on molecular function.</p
DataSheet_2_The analysis of lysine succinylation modification reveals the mechanism of oxybenzone damaging of pakchoi (Brassica rapa L. ssp. chinensis).docx
Oxybenzone (OBZ), one of a broad spectrum of ultraviolet (UV) absorbents, has been proven to be harmful to both plants and animals, while omics analysis of big data at the molecular level is still lacking. Lysine succinylation (Ksuc) is an important posttranslational modification of proteins that plays a crucial role in regulating the metabolic network in organisms under stress. Here, we report the changes in intracellular Ksuc modification in plants under OBZ stress. A total of 1276 succinylated sites on 507 proteins were identified. Among these sites, 181 modified proteins were hypersulfinylated/succinylated in OBZ-stressed pakchoi leaves. Differentially succinylated proteins (DSPs) are distributed mainly in the chloroplast, cytoplasm, and mitochondria and are distributed mainly in primary metabolic pathways, such as reactive oxygen species (ROS) scavenging, stress resistance, energy generation and transfer, photosynthetic carbon fixation, glycolysis, and the tricarboxylic acid (TCA) cycle. Comprehensive analysis shows that Ksuc mainly changes the carbon flow distribution, enhances the activity of the antioxidant system, affects the biosynthesis of amino acids, and increases the modification of histones. The results of this study first showed the profiling of the Kusc map under OBZ treatment and proposed the adaptive mechanism of pakchoi in response to pollutants and other abiotic stresses at the posttranslational level, which revealed the importance of Ksuc in the regulation of various life activities and provides a reference dataset for future research on molecular function.</p
Ring-opening polymerization of lactide using salen–aluminum complexes bearing Schiff-base ligands derived from <i>cis</i>-1,2-cyclohexanediamine
<p>Three aluminum complexes supported by salen ligands derived from <i>cis</i>-1,2-cyclohexanediamine and salicylaldehyde derivatives were synthesized. They were characterized by <sup>1</sup>H, <sup>13</sup>C NMR spectra, and elemental analysis. X-ray diffraction analysis revealed that aluminum was in distorted square pyramidal geometry in <b>2</b>. These complexes were employed as catalysts for the ring-opening polymerization (ROP) of L-lactide and <i>rac</i>-lactide. Complex <b>2</b> showed the highest activity among these complexes with isopropanol for the ROP of L-lactide and <b>3</b> showed the highest stereoselectivity for the ROP of <i>rac</i>-lactide attaining partially isotactic polylactide with a <i>P</i><sub>m</sub> of 0.75. The kinetic data of the polymerization utilizing <b>3</b> as catalyst showed that the polymeric rate was first order to the monomer and catalyst.</p
SHP-1 regulation of basophil differentiation.
<p>Bone marrow cells from WT, <i>mev/mev</i>, <i>Kit<sup>W-sh</sup></i>, and <i>mev</i>/<i>Kit<sup>W-sh</sup></i> mice were cultured in IL-3-containing medium for specified periods of time and the percentage of mature mast cells and basophils were determined. Basophils and mast cells were identified by surface markers CD49b and c-Kit, respectively, in addition to FcεRI by FACS. (<b>A</b>). Percentage of mature basophils and mast cells in different groups on day 8. (<b>B</b>). Kinetics of basophil differentiation from day 0 to day 15. Shown is a representative of three independent experiments with similar results.</p