Fig 5 -

KEGG pathways enriched in DEGs in peanut inoculated with Tsukamurella tyrosinosolvens P9 at 24 h (left) and 72 h (right).</p

Effect of <i>Tsukamurella tyrosinosolvens</i> P9 on the expression of lipid metabolism, photosynthesis, carbon and nitrogen metabolism and transporter family in peanut roots.

Effect of Tsukamurella tyrosinosolvens P9 on the expression of lipid metabolism, photosynthesis, carbon and nitrogen metabolism and transporter family in peanut roots.</p

S1 Fig -

Plots of differential gene volcanoes in peanut roots inoculated with Tsukamurella tyrosinosolvens P9 for 24 h (left) or 72 h (right). Green and red dots indicate down-regulated and up-regulated differentially expressed genes (DEGs), respectively, and blue dots represent genes with no significant expression differences. (TIF)</p

Effect of <i>Tsukamurella tyrosinosolvens</i> strains on the expression of genes related to signal transduction in peanut seedlings.

Effect of Tsukamurella tyrosinosolvens strains on the expression of genes related to signal transduction in peanut seedlings.</p

Effect of <i>Tsukamurella tyrosinosolvens</i> P9 strain on the expression of hormone synthesis in peanut roots.

Effect of Tsukamurella tyrosinosolvens P9 strain on the expression of hormone synthesis in peanut roots.</p

Effect of <i>Tsukumarella tyrosinosolvens</i> P9 on the expression of genes related to pectin degradation in peanut root cell walls.

Effect of Tsukumarella tyrosinosolvens P9 on the expression of genes related to pectin degradation in peanut root cell walls.</p

Fig 3 -

Venn diagram of differential gene volcanoes in peanut roots inoculated with Tsukamurella tyrosinosolvens P9 for 24 h (left) or 72 h (right). The non-overlapping region was the number of unique DEGs of peanut inoculated with P9 strain, and the overlapping region was the number of common DEGs.</p

Growth status of peanut inoculated with <i>Tsukamurella tyrosinosolvens</i> strain P9 after 24 and 72 h.

Growth status of peanut inoculated with Tsukamurella tyrosinosolvens strain P9 after 24 and 72 h.</p

The differential gene expression verified by RT-qPCR.

The left is the DEGs at 24 h after inoculation; the right is the DEGs at 72 h after inoculation. Among them, LOC112703750 encodes polygalacturonase, LOC112777310 encodes trans-cinnamate 4-monooxygenase, LOC112720495 encodes caffeoyl-CoA O-methyltransferase, LOC112795729 encodes PetF, LOC112741560 encodes chalcone synthase-like, LOC112741528 encodes ent-kaurenoic acid monooxygenase, LOC112727194 encodes indole-3-pyruvate monooxygenase, LOC112802019 encodes aminocyclopropanecarboxylate oxidase, LOC112737957 encodes L-tryptophan decarboxylase, novel.668 encodes starch synthase, LOC112783179 encodes ribulose-phosphate 3-epimerase, LOC112718386 encodes ERF1, LOC112747725 encodes L-ascorbate peroxidase, LOC112785663 encodes Nrt, LOC114924223 and LOC112705586 encode cytokinin dehydrogenase, LOC112790626 encodes pectinesterase, LOC112766121 encodes glucuronokinase, LOC112726583 encodes L-gulonolactone oxidase, LOC114925084 encodes 12-oxoophytodienoic acid reductase, LOC112728121 encodes D-glycerate 3-kinase, novel.1265 encodes glycerol-3- phosphate acyltransferase, LOC112726696 encodes CaM4, LOC112747667 encodes DNA methyltransferase 1.</p

Principal component analysis (PCA) of peanut.

CK1 and CK2 are control groups at 24 and 72 h, respectively, and T1-P9 and T2-P9 are peanut groups inoculated with P9 for 24 and 72 h, respectively. Each treatment includes three replicates. (TIF)</p