Enhanced Biocompatibility and Biostability of CdTe Quantum Dots by Facile Surface-Initiated Dendritic Polymerization

The synthesis of stable, low toxic, multifunctional, and water-soluble quantum dots (QDs) is of crucial importance for nanobiotechnology. An in situ anionic ring-opening polymerization strategy was successfully employed to grow multihydroxyl hyperbranched polyglycerol (HPG) from surfaces of aqueous synthesized QDs directly, affording multifunctional CdTe@HPG nanohybrids. The grafted HPG content can be adjusted from about 25 to 80 wt % by manipulating the feed ratio of glycidol monomer to QDs. The resultant CdTe@HPGs still show strong fluorescence and well water-solubility, and can conjugate functional biomolecules (e.g., amino acids) with their multiple reactive hydroxyls. Cytotoxicity measurements reveal that the CdTe@HPGs are much less toxic than the pristine QDs in human lung cancer cells SPCAI and more grafted HPG leads to less toxicity, due to the envelope of biocompatible HPG on QDs. It was found that the pristine QDs were unstable and their fluorescence decreased greatly or was even completed quenched after 24 h in SPCAI cells, whereas the QD@HPGs still exhibited strong fluorescence. This report opens the door for using in situ controlled/living polymerization to tailor QDs with biocompatible dendritic polymers readily and casts a light for obtaining robust nontoxic functionalized QDs and applying them in vitro and in vivo

Synthesis and Evaluation of Phenylalanine-Modified Hyperbranched Poly(amido amine)s as Promising Gene Carriers

Hyperbranched poly(amido amine) (HPAMAM), which is structurally analogous to PAMAM dendrimers, has been proposed to be an effective agent for gene delivery. The facile synthesis of HPAMAM with scalable productivity by one-pot polymerization of monomers of methyl acrylate (MA) and diethylenetriamine (DETA) has been set up previously. In this study, the HPAMAM was further modified on the terminal amino groups with phenylalanine to various degrees (HPAMAM-PHE30, PHE45, PHE60). We showed that HPAMAM and HPAMAM-PHEs were all able to form complexes with plasmid DNA (pDNA) at various mass ratios. The cytotoxicity and transfection efficiencies of these polymers were evaluated in SMMC-7721 and COS-7 cell lines. The PHE modifications affected the cell transfection efficiency significantly. The HPAMAM-PHE60 was the most efficient, with transfection activities consistently higher than the commercial transfection reagent PEI. Our study demonstrated that HPAMAM-PHEs may be good new materials for gene delivery and other applications because of its large-scale availability, economical cost, and low toxicity

High-Quality and Water-Soluble Near-Infrared Photoluminescent CdHgTe/CdS Quantum Dots Prepared by Adjusting Size and Composition

In this article, near-infrared (NIR) CdHgTe alloyed quantum dots (QDs) were directly synthesized in water by heating a mixed solution of CdCl2, Hg(ClO4)2 and NaHTe in the presence of thiol stabilizers. The CdHgTe QDs exhibit photoluminescence (PL) ranging from 600 to 830 nm that can be tuned by size and composition. The quantum yields (QYs) of QDs were about 20−50%, associated with their emission wavelength and composition. Compared to other reported NIR QDs such as CdTe/CdHgTe and InAs, the as-prepared CdHgTe alloyed QDs have much narrower emission spectra, and their full widths at half-maximum (fwhm) are only 60−80 nm. Characterization by HRTEM and XRD showed that the CdHgTe QDs have good monodispersity and a nice crystal structure. To improve the photostability and reduce the cytotoxity of the CdHgTe QDs, a CdS nanocrystal shell was added to the surface of the CdHgTe QD core. Furthermore, the CdHgTe/CdS core/shell QDs were successfully applied for the imaging of living animals. Our preliminary results illustrate that our synthesis procedure is very simple and inexpensive and that the as-prepared products CdHgTe/CdS core/shell QDs are water-soluble and photostable and will be an alternative probe in the imaging of living animals

DataSheet_1_Sustained Drug Release From Liposomes for the Remodeling of Systemic Immune Homeostasis and the Tumor Microenvironment.pdf

Myeloid Derived Suppressor Cells (MDSCs) play important roles in constituting the immune suppressive environment promoting cancer development and progression. They are consisted of a heterogeneous population of immature myeloid cells including polymorphonuclear MDSC (PMN-MDSC) and monocytes MDSC (M-MDSC) that are found in both the systemic circulation and in the tumor microenvironment (TME). While previous studies had shown that all-trans retinoic acid (ATRA) could induce MDSC differentiation and maturation, the very poor solubility and fast metabolism of the drug limited its applications as an immune-modulator for cancer immunotherapy. We aimed in this study to develop a drug encapsulated liposome formulation L-ATRA with sustained release properties and examined the immuno-modulation effects. We showed that the actively loaded L-ATRA achieved stable encapsulation and enabled controlled drug release and accumulation in the tumor tissues. In vivo administration of L-ATRA promoted the remodeling of the systemic immune homeostasis as well as the tumor microenvironment. They were found to promote MDSCs maturation into DCs and facilitate immune responses against cancer cells. When used as a single agent treatment, L-ATRA deterred tumor growth, but only in immune-competent mice. In mice with impaired immune functions, L-ATRA at the same dose was not effective. When combined with checkpoint inhibitory agents, L-ATRA resulted in greater anti-cancer activities. Thus, L-ATRA may present a new IO strategy targeting the MDSCs that needs be further explored for improving the immunotherapy efficacy in cancer.</p

Gyroscope-Structured Triboelectric Nanogenerator for Harvesting Multidirectional Ocean Wave Energy

Wave motion in the ocean can generate plentiful energy, but it is difficult to harvest wave energy for practical use because of the low frequency and random directional characteristics of wave motion. In this paper, a gyroscope-structured triboelectric nanogenerator (GS-TENG) is proposed for harvesting multidirectional ocean wave energy. Its inner and outer generation units can operate independently in different directions, and they all adopt the friction mode of surface contact. While realizing noninterference multidirectional energy harvesting, the power generation area is increased. In the experiments, under acceleration of 6 m/s2 with variations in excitation angle, the GS-TENG can output direct currents of 0.8–3.2 μA, and the open-circuit voltages of the inner and outer generation units can reach 730 and 160 V, respectively. When the devices are networked and placed in the water, the electrical energy generated by the GS-TENGs can enable commercial thermometers to operate normally. The attenuation of direct-current output by the GS-TENG in the experiment of 30 days in water is about 8%, which verifies the good durability of the device in the water environment. Therefore, the GS-TENG has excellent application prospects in the wave energy harvesting field

Characteristics of the studies included in this meta-analysis.

<p>WHO: World Health Organization; ADA: America Diabetes Association; NDDG: National Diabetes Data Group; NCEP-ATP III: National Cholesterol Education Program Adult Treatment Panel; IDF: International Diabetes Federation.</p

Gyroscope-Structured Triboelectric Nanogenerator for Harvesting Multidirectional Ocean Wave Energy

Wave motion in the ocean can generate plentiful energy, but it is difficult to harvest wave energy for practical use because of the low frequency and random directional characteristics of wave motion. In this paper, a gyroscope-structured triboelectric nanogenerator (GS-TENG) is proposed for harvesting multidirectional ocean wave energy. Its inner and outer generation units can operate independently in different directions, and they all adopt the friction mode of surface contact. While realizing noninterference multidirectional energy harvesting, the power generation area is increased. In the experiments, under acceleration of 6 m/s2 with variations in excitation angle, the GS-TENG can output direct currents of 0.8–3.2 μA, and the open-circuit voltages of the inner and outer generation units can reach 730 and 160 V, respectively. When the devices are networked and placed in the water, the electrical energy generated by the GS-TENGs can enable commercial thermometers to operate normally. The attenuation of direct-current output by the GS-TENG in the experiment of 30 days in water is about 8%, which verifies the good durability of the device in the water environment. Therefore, the GS-TENG has excellent application prospects in the wave energy harvesting field

Gyroscope-Structured Triboelectric Nanogenerator for Harvesting Multidirectional Ocean Wave Energy

Wave motion in the ocean can generate plentiful energy, but it is difficult to harvest wave energy for practical use because of the low frequency and random directional characteristics of wave motion. In this paper, a gyroscope-structured triboelectric nanogenerator (GS-TENG) is proposed for harvesting multidirectional ocean wave energy. Its inner and outer generation units can operate independently in different directions, and they all adopt the friction mode of surface contact. While realizing noninterference multidirectional energy harvesting, the power generation area is increased. In the experiments, under acceleration of 6 m/s2 with variations in excitation angle, the GS-TENG can output direct currents of 0.8–3.2 μA, and the open-circuit voltages of the inner and outer generation units can reach 730 and 160 V, respectively. When the devices are networked and placed in the water, the electrical energy generated by the GS-TENGs can enable commercial thermometers to operate normally. The attenuation of direct-current output by the GS-TENG in the experiment of 30 days in water is about 8%, which verifies the good durability of the device in the water environment. Therefore, the GS-TENG has excellent application prospects in the wave energy harvesting field