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<p>Cell scratch test and Transwell were used to measure the migration abilities of HSVSMCs. NC = Negative control group, only control siRNA transfected; GAS5(-) = lncRNA-GAS5 knockdown group transfected with silence siRNA. <b>A:</b>Cell scratch test was used to measure the migration abilities of HSVSMCs. The results showed that the HSVSMCs have the best migration abilities in the first 24 hours. Values are mean±SE, N = 4. <b>B:</b> The migration abilities of HSVSMCs measured by Transwell. After transfected by lncRNA-GAS5 siRNA for 48 hours, the HSVSMCs were passage into the Transwell Inserts. Then 4 hours, 7 hours, 10 hours later, the migration HSVSMCs were photographed and counted, respectively. Knockdown of lncRNA-GAS5 expression promotes migration of HSVSMCs. Optical microscope images under 200x magnification. <b>C:</b> The migration abilities of HSVSMCs were reflected indirectly by the new migration cells counting with Transwell. Silencing of lncRNA-GAS5 expression increses migration ability of HSVSMCs. Values are mean±SE, N = 10; *, P<0.05.</p

Simulator for Virtual Instrumentation Courses

Import 05/08/2014Tato bakalářská práce se zabývá návrhem a realizací simulátoru pro výuku virtuální instrumentace. Tento simulátor je zkonstruován na bázi mikrokontroléru, který obstarává hlavní funkčnost celého zařízení a je naprogramován tak, aby uměl vykonávat funkce napětím řízeného oscilátoru, napětím řízeného PWM, simulátoru servomotoru a generátoru impulzů. Částí této práce je i vytvoření řídícího programu, který komunikuje s mikrokontrolérem pomocí sériové linky a umožňuje parametrizaci a nastavení funkce simulátoru.This bechelor thesis describes the design and implementation of simulator for the teaching of virtual instrumentation courses. This simulator is based on microcontroller, which performs the main functionality of the device and is programmed to be able to perform the functions of a voltage-controlled oscillator, voltage controlled PWM, the simulator of servo motor and a pulse generator. Part of this work is the creation of a application that communicates with a microcontroller via a serial line and allows the configuration and settings of the simulator.450 - Katedra kybernetiky a biomedicínského inženýrstvívelmi dobř

C–C Stretching Raman Spectra and Stabilities of Hydrocarbon Molecules in Natural Gas Hydrates: A Quantum Chemical Study

The presence of specific hydrocarbon gas molecules in various types of water cavities in natural gas hydrates (NGHs) are governed by the relative stabilities of these encapsulated guest molecule–water cavity combinations. Using molecular quantum chemical dispersion-corrected hybrid density functional computations, the interaction (Δ<i>E</i>_{host<i>‑</i>–guest}) and cohesive energies (Δ<i>E</i>_coh), enthalpies, and Gibbs free energies for the complexes of host water cages and hydrocarbon guest molecules are calculated at the ωB97X-D/6-311++G­(2d,2p) level of theory. The zero-point energy effect of Δ<i>E</i>_host–guest and Δ<i>E</i>_coh is found to be quite substantial. The energetically optimal host–guest combinations for seven hydrocarbon gas molecules (CH₄, C₂H₆, C₃H₆, C₃H₈, C₄H₈, i-C₄H₁₀, and <i>n</i>-C₄H₁₀) and various water cavities (D, ID, T, P, H, and I) in NGHs are found to be CH₄@D, C₂H₆@T, C₃H₆@T, C₃H₈@T, C₄H₈@T/P/H, i-C₄H₁₀@H, and <i>n</i>-C₄H₁₀@H, as the largest cohesive energy magnitudes will be obtained with these host–guest combinations. The stabilities of various water cavities enclosing hydrocarbon molecules are evaluated from the computed cohesive Gibbs free energies: CH₄ prefers to be trapped in a ID cage; C₂H₆ prefer T cages; C₃H₆ and C₃H₈ prefer T and H cages; C₄H₈ and i-C₄H₁₀ prefer H cages; and <i>n</i>-C₄H₁₀ prefer I cages. The vibrational frequencies and Raman intensities of the C–C stretching vibrational modes for these seven hydrocarbon molecules enclosed in each water cavity are computed. A blue shift results after the guest molecule is trapped from gas phase into various water cages due to the host–guest interactions between the water cage and hydrocarbon molecule. The frequency shifts to the red as the radius of water cages increases. The model calculations support the view that C–C stretching vibrations of hydrocarbon molecules in the water cavities can be used as a tool to identify the types of crystal phases and guest molecules in NGHs

Sequence Effect on the Formation of DNA Minidumbbells

The DNA minidumbbell (MDB) is a recently identified non-B structure. The reported MDBs contain two TTTA, CCTG, or CTTG type II loops. At present, the knowledge and understanding of the sequence criteria for MDB formation are still limited. In this study, we performed a systematic high-resolution nuclear magnetic resonance (NMR) and native gel study to investigate the effect of sequence variations in tandem repeats on the formation of MDBs. Our NMR results reveal the importance of hydrogen bonds, base–base stacking, and hydrophobic interactions from each of the participating residues. We conclude that in the MDBs formed by tandem repeats, C–G loop-closing base pairs are more stabilizing than T–A loop-closing base pairs, and thymine residues in both the second and third loop positions are more stabilizing than cytosine residues. The results from this study enrich our knowledge on the sequence criteria for the formation of MDBs, paving a path for better exploring their potential roles in biological systems and DNA nanotechnology

First Generation Students Group Shot

First Generation Students Group Shot SCM Cover, Drahmann Center, CML, Top L to R: Juan Carlos Esquivel, Pablo Madriz, Nicholas Rosiles, Juan Yanez, MiddleL to R: Elizabeth Ojeda, Cynthia Naccarato, Veronica Ramirez, Ruth Lazaro, Maria Ochoa, Yuridia Estrada, BottomL to R: Everardo Leon, Carmen Rivera, Loren ""Gabby"" Reye

Cephalosporolide B Serving as a Versatile Synthetic Precursor: Asymmetric Biomimetic Total Syntheses of Cephalosporolides C, E, F, G, and (4-OMe-)G

Cephalosporolide B (Ces-B) was efficiently synthesized and exploited for the first time as a versatile biomimetic synthetic precursor for the chemical syntheses of not only cephalosporolides C, G, and (4-OMe-) G via a challenging diastereoselective oxa-Michael addition but also the structurally unprecedented cephalosporolides E and F via a novel biomimetic ring-contraction rearrangement. These findings provide the first direct chemical evidence that Ces-B may be the true biosynthetic precursor of cephalosporolides

Median duration of the four trial designs by accrual rate.

<p>The first and third quartiles are shown in parentheses.</p

Mean square error of the estimated MTD for each of the four models and nine scenarios when the time to DLT is generated from a proportional hazards model with Weibull baseline hazard rate with parameters <i>κ</i> = 0.5 (left panel), λ = <i>κ</i> = 1.0 (middle panel), and <i>κ</i> = 1.5 (right panel).

<p>Mean square error of the estimated MTD for each of the four models and nine scenarios when the time to DLT is generated from a proportional hazards model with Weibull baseline hazard rate with parameters <i>κ</i> = 0.5 (left panel), λ = <i>κ</i> = 1.0 (middle panel), and <i>κ</i> = 1.5 (right panel).</p

Average bias of the estimated MTD for each of the four models and nine scenarios when the time to DLT is generated from a non-proportional hazards model.

<p><i>β</i>₂ = 0.5 (left panel), <i>β</i>₂ = 2.0 (right panel).</p

Analysis of Hand and Wrist Postural Synergies in Tolerance Grasping of Various Objects

<div><p>Human can successfully grasp various objects in different acceptable relative positions between human hand and objects. This grasp functionality can be described as the grasp tolerance of human hand, which is a significant functionality of human grasp. To understand the motor control of human hand completely, an analysis of hand and wrist postural synergies in tolerance grasping of various objects is needed. Ten healthy right-handed subjects were asked to perform the tolerance grasping with right hand using 6 objects of different shapes, sizes and relative positions between human hand and objects. Subjects were wearing CyberGlove attaching motion tracker on right hand, allowing a measurement of the hand and wrist postures. Correlation analysis of joints and inter-joint/inter-finger modules were carried on to explore the coordination between joints or modules. As the correlation between hand and wrist module is not obvious in tolerance grasping, individual analysis of wrist synergies would be more practical. In this case, postural synergies of hand and wrist were then presented separately through principal component analysis (PCA), expressed through the principal component (PC) information transmitted ratio, PC elements distribution and reconstructed angle error of joints. Results on correlation comparison of different module movements can be well explained by the influence factors of the joint movement correlation. Moreover, correlation analysis of joints and modules showed the wrist module had the lowest correlation among all inter-finger and inter-joint modules. Hand and wrist postures were both sufficient to be described by a few principal components. In terms of the PC elements distribution of hand postures, compared with previous investigations, there was a greater proportion of movement in the thumb joints especially the interphalangeal (IP) and opposition rotation (ROT) joint. The research could serve to a complete understanding of hand grasp, and the design, control of the anthropomorphic hand and wrist.</p></div