Nickel-Catalyzed Transformation of Alkene-Tethered Oxime Ethers to Nitriles by a Traceless Directing Group Strategy

Nickel-catalyzed transformation of alkene-tethered oxime ethers to nitriles using a traceless directing group strategy has been developed. A series of alkene-tethered oxime ethers derived from benzaldehyde and cinnamyl aldehyde derivatives were converted into the corresponding benzonitriles and cinnamonitriles in 46–98% yields using the nickel catalyst system. Control experiments showed that the alkene group tethered to an oxygen atom on the oximes via one methylene unit plays a key role as a traceless directing group during the catalysis

Nickel-Catalyzed Hydroarylation of in Situ Generated 1,3-Dienes with Arylboronic Acids Using a Secondary Homoallyl Carbonate as a Surrogate for the 1,3-Diene and Hydride Source

The nickel-catalyzed hydroarylation of 1,3-dienes with arylboronic acids using a secondary homoallyl carbonate as a surrogate for the 1,3-diene and hydride source has been developed. The synthetic strategy allowed an efficient access to a wide array of hydroarylation products in high yields with high functional group compatibility without the use of an external hydride source. Mechanistic experiments indicated that the alkene-directed oxidative addition and subsequent β-hydride elimination would be a critical process in this transformation

Localization of previously reported <i>SZT2</i> variants.

Red and blue font indicates the variants in patient 1 and 2, respectively. Green font indicates null variants. Black font indicates missense or in-frame deletion variants.</p

Hyperactivation of mTORC1 signaling under both starved and re-stimulated conditions in the patients.

(A) Levels and phosphorylation status of the indicated proteins in the patients and wild-type subjects were determined by immunoblotting. Cells were re-stimulated with amino acids for 10 min (lane +) and 1 h (lane ++). GAPDH was used as a loading control. WT, wild-type. (B) Quantification of the ratio of p-S6K to S6K and p-S6 to S6 bands. Phosphorylation levels of patient 1 and 2 were significantly higher than those of controls under starved and 10 min stimulated conditions, whereas no significant difference after 1 h. Comparing two patients, patient 2 tended to be more activated than patient 1. *p p < 0.01. (C) Simplified schema of the amino acid-sensing pathway upstream of mTORC1 and the downstream targets. K, I and C represent KPTN, ITFG2 and C12orf66, respectively. They form KICSTOR complex with SZT2. P denotes phosphorylation.</p

Amino acid-insensitive constitutive lysosomal localization of mTOR in the patients.

(A) Cells were starved, or starved and re-stimulated with amino acids for 1 h. The localization of mTOR and LAMP1 was determined by immunofluorescence. Representative images of co-localization of mTOR (red) and LAMP1 (green) in patient 1 and wild type 1 are shown on behalf of each patient and control. Right panels show the enlarged view of co-localizing area. DAPI, nuclear staining (blue). Scale bars, 5 μm. See also S1 Fig for patient 2, wild type 2 and 3. (B) Quantification of co-localization between mTOR and LAMP1. Co-localization levels of patient 1 and 2 were significantly higher than those of controls under each condition. * p p < 0.01.</p

Supplemental_table_3 - Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain

Supplemental_table_3 for Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain by Takahiro Kitagawa, Yoji Ogura, Yoshiomi Kobayashi, Yoshiyuki Takahashi, Yoshiro Yonezawa, Kodai Yoshida, Yohei Takahashi, Akimasa Yasuda, Yoshio Shinozaki and Jun Ogawa in Global Spine Journal</p

Supplemental_table_2 - Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain

Supplemental_table_2 for Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain by Takahiro Kitagawa, Yoji Ogura, Yoshiomi Kobayashi, Yoshiyuki Takahashi, Yoshiro Yonezawa, Kodai Yoshida, Yohei Takahashi, Akimasa Yasuda, Yoshio Shinozaki and Jun Ogawa in Global Spine Journal</p

Microsatellite instability-high is rare events in refractory pediatric solid tumors

Microsatellite instability (MSI)-high status is associated with good responsiveness to immune checkpoint inhibitors. Although MSI-high status has been actively investigated in pediatric brain tumors, studies of other pediatric solid tumors are lacking. Among 334 consecutive pediatric patients with solid tumors, we retrospectively analyzed formalin-fixed paraffin-embedded tumor tissues of 36 of 74 patients (49%) who died of disease. We assessed the MSI status in these tissues using five multiplexed markers. The results revealed that none of the patients had an MSI-high status. These results indicate that MSI-high status is a rare event in pediatric patients with refractory/relapsed solid tumors. Supplemental data for this article is available online at https://doi.org/10.1080/08880018.2021.1998266</p

Supplemental_table_1 - Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain

Supplemental_table_1 for Improvement of Lower Back Pain in Lumbar Spinal Stenosis After Decompression Surgery and Factors That Predict Residual Lower Back Pain by Takahiro Kitagawa, Yoji Ogura, Yoshiomi Kobayashi, Yoshiyuki Takahashi, Yoshiro Yonezawa, Kodai Yoshida, Yohei Takahashi, Akimasa Yasuda, Yoshio Shinozaki and Jun Ogawa in Global Spine Journal</p

Supplemental Figure Legend for Supplemental Figure 1 from Detection of an Immunogenic HERV-E Envelope with Selective Expression in Clear Cell Kidney Cancer

Supplemental Figure Legend for Supplemental Figure 1</p