Cost-Effective Screening of Antimicrobial Performance of Multiple Metal–Organic Frameworks via a Droplet-Based Batch Synthesis Platform

Metal–organic frameworks (MOFs) have recently been widely used in antimicrobial fields due to their adjustable composition and morphology. The inherent properties of MOFs (e.g., their shape, size, and metal element) are highly related to their antibacterial performance. The traditional beaker-based synthesis system to evaluate antibacterial properties of MOFs often leads to high reagent costs and environmental pollution. Here, a low-cost droplet-based microscale synthesis platform for better and economical screening of antimicrobial performance of MOFs is demonstrated. Multiple MOFs (Zn-, Co-, and Cu-MOFs) with controlled morphologies are successfully one-click fabricated through such a droplet-based synthesis platform. Further antimicrobial performance screening (Staphylococcus aureus and Escherichia coli) of the above MOFs indicates that the Co-MOFs show better antibacterial effects than Zn-MOFs and Cu-MOFs. Such integration of droplet array synthesis and screening can greatly reduce the cost and time, especially for parallel experiments, and provide great potential in guiding the large-scale synthesis of high-performance materials

Cost-Effective Screening of Antimicrobial Performance of Multiple Metal–Organic Frameworks via a Droplet-Based Batch Synthesis Platform

Metal–organic frameworks (MOFs) have recently been widely used in antimicrobial fields due to their adjustable composition and morphology. The inherent properties of MOFs (e.g., their shape, size, and metal element) are highly related to their antibacterial performance. The traditional beaker-based synthesis system to evaluate antibacterial properties of MOFs often leads to high reagent costs and environmental pollution. Here, a low-cost droplet-based microscale synthesis platform for better and economical screening of antimicrobial performance of MOFs is demonstrated. Multiple MOFs (Zn-, Co-, and Cu-MOFs) with controlled morphologies are successfully one-click fabricated through such a droplet-based synthesis platform. Further antimicrobial performance screening (Staphylococcus aureus and Escherichia coli) of the above MOFs indicates that the Co-MOFs show better antibacterial effects than Zn-MOFs and Cu-MOFs. Such integration of droplet array synthesis and screening can greatly reduce the cost and time, especially for parallel experiments, and provide great potential in guiding the large-scale synthesis of high-performance materials

Hydrogel-Functionalized Bandages with Janus Wettability for Efficient Unidirectional Drug Delivery and Wound Care

Chronic wounds have imposed a severe physical and economic burden on the global healthcare system, which are usually treated by the delivery of drugs or bioactive molecules to the wound bed through wound dressings. In this work, we have demonstrated a hydrogel-functionalized bandage with Janus wettability in a bilayer structure to achieve unidirectional drug delivery and multifunctional wound care. The Janus patterned bandage with porous gradient wetting channels on the upper layer is responsible for the unidirectional transport of the drug from the outside to the wound bed (up to 90% drug transport efficiency) while preventing drug diffusion in unwanted directions (<8%). The hydrogel composed of chitosan quaternary ammonium salt (HACC), poly­(vinyl alcohol) (PVA), and poly­(acrylic acid) (PAA) at the bottom layer further functionalized such a bandage with biocompatibility, excellent antibacterial properties, and hemostatic ability to promote wound healing. Especially, the hydrogel-functionalized bandage with Janus wettability exhibits excellent mechanical flexibility (∼198% strain), which can comply well with skin deformation (stretching, bending, or twisting) and maintain unidirectional drug delivery behavior without any leakage. The in vivo full-thickness skin wound model confirms that the hydrogel-functionalized bandage can significantly facilitate epithelialization and collagen deposition and improve drug delivery efficiency, thus promoting wound closure and healing (the wound healing ratio was 98.10% at day 15). Such a synergistic strategy of unidirectional drug delivery and multifunctional wound care provides a more efficient, economical, and direct method to promote wound healing, which could be used as a potential high-performance wound dressing for clinical application

Presentation_2_Safety-oriented planning of expressway truck service areas based on driver demand.pdf

The rapid development of the economy has promoted the growth of freight transportation. The truck service areas on expressways, as the main places for truck drivers to rest, play an important role in ensuring the driving safety of trucks. If these service areas are constructed densely or provide a plentiful supply of parking areas, they are costly to construct. However, if the distance between two adjacent truck service areas is very large or the number of truck parking spaces in service areas is small, the supply will fail to meet the parking needs of truck drivers. In this situation, the continuous working time of truck drivers will be longer, and this is likely to cause driver fatigue and even traffic accidents. To address these issues, this paper established a non-linear optimization model for truck service area planning of expressways to optimize truck driving safety. An improved genetic algorithm is proposed to solve the model. A case study of a 215.5-kilometers-length section of the Guang-Kun expressway in China was used to demonstrate the effectiveness of the model and algorithm. As validated by this specific case, the proposed model and solution algorithm can provide an optimal plan for the layout of truck service areas that meet the parking needs of truck drivers while minimizing the service loss rate. The research results of this paper can contribute to the construction of truck service areas and the parking management of trucks on expressways.</p

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

Bioinspired superwettable micropatterns that combine two extreme states of superhydrophobicity and superhydrophilicity with the ability to enrich and absorb microdroplets are suitable for versatile and robust sensing applications. Here we introduce a superwettable microchip that integrates superhydrophobic–superhydrophilic micropatterns and a nanodendritic electrochemical biosensor toward the detection of prostate cancer biomarkers. On the superwettable microchip, the superhydrophobic area could confine the microdroplets in superhydrophilic microwells; such behavior is extremely helpful for reducing the amount of analytical solution. In contrast, superhydrophilic microwells exhibit a high adhesive force toward microdroplets, and the nanodendritic structures can improve probe-binding capacity and response signals, thus greatly enhancing the sensitivity. Sensitive and selective detection of prostate cancer biomarkers including miRNA-375, miRNA-141, and prostate-specific antigen on a single microchip is also achieved. Such a superwettable microchip with high sensitivity, low sample volume, and upside-down detection capability in a single microdroplet shows great potential to fabricate portable devices toward complex biosensing applications

Active Enrichment of Nanoparticles for Ultra-Trace Point-of-Care COVID-19 Detection

Active enrichment can detect nucleic acid at ultra-low concentrations without relatively time-consuming polymerase chain reaction (PCR), which is an important development direction for future rapid nucleic acid detection. Here, we reported an integrated active enrichment platform for direct hand-held detection of nucleic acid of COVID-19 in nanoliter samples without PCR. The platform consists of a capillary-assisted liquid-carrying system for sampling, integrated circuit system for ultrasound output, and cell-phone-based surface-enhanced Raman scattering (SERS) system. Considering the acoustic responsiveness and SERS-enhanced performance, gold nanorods were selected for biomedical applications. Functionalized gold nanorods can effectively capture and enrich biomarkers under ultrasonic aggregation. Such approaches can actively assemble gold nanorods in 1–2 s and achieved highly sensitive (6.15 × 10–13 M) SERS detection of COVID-19 biomarkers in nanoliter (10–7 L) samples within 5 min. We further demonstrated the high stability, repeatability, and selectivity of the platform, and validated its potential for the detection of throat swab samples. This simple, portable, and ultra-trace integrated active enrichment detection platform is a promising diagnostic tool for the direct and rapid detection of COVID-19

Presentation_1_Safety-oriented planning of expressway truck service areas based on driver demand.pdf

The rapid development of the economy has promoted the growth of freight transportation. The truck service areas on expressways, as the main places for truck drivers to rest, play an important role in ensuring the driving safety of trucks. If these service areas are constructed densely or provide a plentiful supply of parking areas, they are costly to construct. However, if the distance between two adjacent truck service areas is very large or the number of truck parking spaces in service areas is small, the supply will fail to meet the parking needs of truck drivers. In this situation, the continuous working time of truck drivers will be longer, and this is likely to cause driver fatigue and even traffic accidents. To address these issues, this paper established a non-linear optimization model for truck service area planning of expressways to optimize truck driving safety. An improved genetic algorithm is proposed to solve the model. A case study of a 215.5-kilometers-length section of the Guang-Kun expressway in China was used to demonstrate the effectiveness of the model and algorithm. As validated by this specific case, the proposed model and solution algorithm can provide an optimal plan for the layout of truck service areas that meet the parking needs of truck drivers while minimizing the service loss rate. The research results of this paper can contribute to the construction of truck service areas and the parking management of trucks on expressways.</p

Active Enrichment of Nanoparticles for Ultra-Trace Point-of-Care COVID-19 Detection

Active enrichment can detect nucleic acid at ultra-low concentrations without relatively time-consuming polymerase chain reaction (PCR), which is an important development direction for future rapid nucleic acid detection. Here, we reported an integrated active enrichment platform for direct hand-held detection of nucleic acid of COVID-19 in nanoliter samples without PCR. The platform consists of a capillary-assisted liquid-carrying system for sampling, integrated circuit system for ultrasound output, and cell-phone-based surface-enhanced Raman scattering (SERS) system. Considering the acoustic responsiveness and SERS-enhanced performance, gold nanorods were selected for biomedical applications. Functionalized gold nanorods can effectively capture and enrich biomarkers under ultrasonic aggregation. Such approaches can actively assemble gold nanorods in 1–2 s and achieved highly sensitive (6.15 × 10–13 M) SERS detection of COVID-19 biomarkers in nanoliter (10–7 L) samples within 5 min. We further demonstrated the high stability, repeatability, and selectivity of the platform, and validated its potential for the detection of throat swab samples. This simple, portable, and ultra-trace integrated active enrichment detection platform is a promising diagnostic tool for the direct and rapid detection of COVID-19

Active Enrichment of Nanoparticles for Ultra-Trace Point-of-Care COVID-19 Detection

Active enrichment can detect nucleic acid at ultra-low concentrations without relatively time-consuming polymerase chain reaction (PCR), which is an important development direction for future rapid nucleic acid detection. Here, we reported an integrated active enrichment platform for direct hand-held detection of nucleic acid of COVID-19 in nanoliter samples without PCR. The platform consists of a capillary-assisted liquid-carrying system for sampling, integrated circuit system for ultrasound output, and cell-phone-based surface-enhanced Raman scattering (SERS) system. Considering the acoustic responsiveness and SERS-enhanced performance, gold nanorods were selected for biomedical applications. Functionalized gold nanorods can effectively capture and enrich biomarkers under ultrasonic aggregation. Such approaches can actively assemble gold nanorods in 1–2 s and achieved highly sensitive (6.15 × 10–13 M) SERS detection of COVID-19 biomarkers in nanoliter (10–7 L) samples within 5 min. We further demonstrated the high stability, repeatability, and selectivity of the platform, and validated its potential for the detection of throat swab samples. This simple, portable, and ultra-trace integrated active enrichment detection platform is a promising diagnostic tool for the direct and rapid detection of COVID-19