Additional file 3 of NKD2 is correlated with the occurrence, progression and prognosis of thyroid carcinoma

Additional file 3. The original results of western blot

Additional file 2 of NKD2 is correlated with the occurrence, progression and prognosis of thyroid carcinoma

Additional file 2: Table S2. GSEA result

Additional file 1 of NKD2 is correlated with the occurrence, progression and prognosis of thyroid carcinoma

Additional file 1: Table S1. Clinical features of thyroid cancer patients

Puma GPS movement points and attributes

This file includes GPS points collected for 16 pumas collected from 2011 through 2013 in the Santa Cruz Mountains of California. Refer to readme file for column heading descriptions

DataSheet1_A characterization method for equivalent elastic modulus of rock based on elastic strain energy.PDF

Energy is an internal variable during rock deformation and failure, and its dissipation and conversion law can reflect the rock’s internal damage and deterioration state. Analysis of rock deformation and failure process from the perspective of energy is helpful to deeply understand the mechanism of rock damage, fracture and instability failure, and has important theoretical and practical significance for the stability evaluation and support control of surrounding rock. In this study, through single cyclic loading and unloading (SCLU) experiments, cyclic triaxial loading and unloading (CTLU) experiments and conventional triaxial compression (CTC) experiments, the equivalent elastic modulus method based on elastic strain energy is proposed to analyze the energy conversion of rock. The results show that the error of the elastic strain energy calculated by the strain energy formula method is generally higher than 10% with the secant and tangent modulus of the loading and unloading curve as input parameters. Taking the equivalent elastic modulus proposed in this study as an input parameter, more accurate elastic strain energy can be obtained by the strain energy formula. During the rock failure process, the equivalent elastic modulus shows a three-stage characteristic of increase, steady and decrease. The equivalent elastic modulus can be estimated by the quadratic function between the equivalent elastic modulus and confining pressure and axial strain. Under the same deformation and deviatoric stress, the elastic strain energy stored in rock increases with increasing confining pressure. The local maximum energy dissipation rate corresponds to stress drop, and the peak energy dissipation rate appears near the peak strength. High energy dissipation mainly occurs in a short time after peak strength, and energy release and dissipation are more sudden and severe under high confining pressure.</p

Table1_A characterization method for equivalent elastic modulus of rock based on elastic strain energy.DOCX

Energy is an internal variable during rock deformation and failure, and its dissipation and conversion law can reflect the rock’s internal damage and deterioration state. Analysis of rock deformation and failure process from the perspective of energy is helpful to deeply understand the mechanism of rock damage, fracture and instability failure, and has important theoretical and practical significance for the stability evaluation and support control of surrounding rock. In this study, through single cyclic loading and unloading (SCLU) experiments, cyclic triaxial loading and unloading (CTLU) experiments and conventional triaxial compression (CTC) experiments, the equivalent elastic modulus method based on elastic strain energy is proposed to analyze the energy conversion of rock. The results show that the error of the elastic strain energy calculated by the strain energy formula method is generally higher than 10% with the secant and tangent modulus of the loading and unloading curve as input parameters. Taking the equivalent elastic modulus proposed in this study as an input parameter, more accurate elastic strain energy can be obtained by the strain energy formula. During the rock failure process, the equivalent elastic modulus shows a three-stage characteristic of increase, steady and decrease. The equivalent elastic modulus can be estimated by the quadratic function between the equivalent elastic modulus and confining pressure and axial strain. Under the same deformation and deviatoric stress, the elastic strain energy stored in rock increases with increasing confining pressure. The local maximum energy dissipation rate corresponds to stress drop, and the peak energy dissipation rate appears near the peak strength. High energy dissipation mainly occurs in a short time after peak strength, and energy release and dissipation are more sudden and severe under high confining pressure.</p

CitSci.org extensible metadata schema related to a simple field observation.

<p>An observer measures the turbidity of a stream using a Secchi disk. We capture the protocol used to measure turbidity (Secchi disk) in a metadata table (TBL_Metadata) and relate it to the observation (TBL_Observations). This extensible structure allows us to capture any type of metadata, and relate it to observations in addition to other entities, such as projects, individuals, media, etc.</p

Predator Sample by OTU

Sequence counts in individual scat samples matching reference sequences of extant carnivores in the Santa Cruz Mountains. Scat samples were collected by citizen scientists on trails in seven Midpeninsula Open Space preserves and were all less than one week old

Prey Sample by OTU

Sequence counts in individual scat samples matching reference sequences of extant vertebrates (potential prey species of mesocarnivores) in the Santa Cruz Mountains. Scat samples were collected by citizen scientists on trails in seven Midpeninsula Open Space preserves and were all less than one week old

Image_1.TIF

<p>The dermis of human skin contains large numbers of fibroblasts that are responsible for the production of the extracellular matrix (ECM) that supporting skin integrity, elasticity and wound healing. Previously, an in vivo study demonstrated that dermal fibroblasts siting in the lower dermis are capable to convert into skin adipose layer and hence fibroblast lipogenesis may vary the structure and elasticity of dermis. In the present study, Hs68 human dermal fibroblasts were utilized as an in vitro model to study the lipogenesis via using adipogenic differentiation medium (ADM). Baicalein, isolated from Scutellaria baicalensis, is one of the flavonoids to inhibit adipocyte differentiation due to high antioxidant activity in vitro. In order to develop a suitable formulation for baicalein (a poorly water-soluble drug), soybean phosphatidylcholine (SPC) was used to prepare baicalein-loaded liposomes to enhance drug bioavailability. Our results demonstrated that liposome-encapsulated baicalein protected cell viability and increased cellular uptake efficiency of Hs68 fibroblasts. Lipid accumulation, triglyceride synthesis and gene expressions of lipogenesis enzymes (FABP4 and LPL) were significantly increased in ADM-stimulated Hs68 fibroblasts but subsequently suppressed by liposome-encapsulated baicalein. In addition, ADM-induced TNF-α expression and related inflammatory factors was down-regulated by liposome-encapsulated baicalein. Through ADM-induced lipogenesis, the protein expression of elastin, type I and type III collagens increased remarkably, whereas liposome-encapsulated baicalein can down-regulate ADM-induced ECM protein synthesis. Taken together, we found that liposome-encapsulated baicalein can inhibit ADM-induced lipid accumulation and ECM formation in Hs68 fibroblasts through the suppression of lipogenesis enzymes and inflammatory responses. Liposome-encapsulated baicalein may have the potential to improve wound healing and restore skin structure after skin injury.</p