RMSF in simulations of CRL.

<p>The data were indicated the flexibility of CRL calculated from the last 6 ns of simulations in pure water (black), in binary DMF solution (blue), and in ternary DMF/trehalose solution (red).</p

Fluorescence emission changes of CRL.

<p>Plots of fluorescence intensity at an emission wavelength of 335 nm and an excitation wavelength of 280 nm versus DMF concentration at different trehalose concentrations.</p

Mean number of hydrogen bonds between different molecules in different solutions ^a.

<p>Mean number of hydrogen bonds between different molecules in different solutions <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152275#t002fn001" target="_blank">^a</a>.</p

Radial distribution functions.

<p>RDFs for water oxygen, DMF carbon, and trehalose hydroxyl oxygen around the center of mass (COM) of the protein as a function of the distance <i>r</i> from COM of the protein in binary DMF solution (A) and a ternary DMF/trehalose solution (B) over the last 6 ns of the simulation.</p

Total RMSF (sum of RMSF per residue) of CRL averaged over the last 6 ns of each simulation in three solvents.

<p>Total RMSF (sum of RMSF per residue) of CRL averaged over the last 6 ns of each simulation in three solvents.</p