Additional file 1 of Novel PAK3 gene missense variant associated with two Chinese siblings with intellectual disability: a case report

Additional file 1 Table S1. Stability results of PAK3 and variant, produced by Molecular Operating Environment (MOE). Table S2. The summary of variants consistent with the inheritance model other than the PAK3 gene in this family from the trio-WES data

Supplementary materials. Prognostic value of PD-L1 expression combined with hypoxia-associated immunosuppression in hepatocellular carcinoma

Supplemental Table S1. The follow-up information of patients with hepatocellular carcinoma.</div

Supplementary materials. Prognostic value of PD-L1 expression combined with hypoxia-associated immunosuppression in hepatocellular carcinoma

Supplemental Figure S1. Identification of consensus clusters by hypoxia-related genes.</div

Two Metal Phosphate Nonlinear Optical Materials Simultaneously Exhibiting Ultraviolet Transparence and a Large Birefringence

For phosphate nonlinear optical (NLO) materials, how to improve their small birefringence is confronted with a great contradiction of their weak optical anisotropy of tetrahedral PO4 groups. Herein, by introducing La3+ with a closed-shell structure and Cd2+ with a d10 electronic configuration, two NLO materials with a large birefringence, namely, La­(PO3)3 (0.040@1064 nm) and β-Cd­(PO3)2 (0.059@1064 nm), have been synthesized by a high-temperature solution method. In particular, for β-Cd­(PO3)2, it possesses the largest birefringence among the known deep-ultraviolet (DUV) phosphates to date, which is attributed to cooperative effects of strong covalence of Cd–O groups and P–O pseudolayers similar to those of a plane. Meanwhile, β-Cd­(PO3)2 exhibits the shortest cutoff edge (<190 nm) among the reported Cd-based inorganic compounds and realizes a balance between DUV transparence and a large birefringence. This insight provides a new opportunity to design high-performance NLO materials using metal phosphates

Two Metal Phosphate Nonlinear Optical Materials Simultaneously Exhibiting Ultraviolet Transparence and a Large Birefringence

For phosphate nonlinear optical (NLO) materials, how to improve their small birefringence is confronted with a great contradiction of their weak optical anisotropy of tetrahedral PO4 groups. Herein, by introducing La3+ with a closed-shell structure and Cd2+ with a d10 electronic configuration, two NLO materials with a large birefringence, namely, La­(PO3)3 (0.040@1064 nm) and β-Cd­(PO3)2 (0.059@1064 nm), have been synthesized by a high-temperature solution method. In particular, for β-Cd­(PO3)2, it possesses the largest birefringence among the known deep-ultraviolet (DUV) phosphates to date, which is attributed to cooperative effects of strong covalence of Cd–O groups and P–O pseudolayers similar to those of a plane. Meanwhile, β-Cd­(PO3)2 exhibits the shortest cutoff edge (<190 nm) among the reported Cd-based inorganic compounds and realizes a balance between DUV transparence and a large birefringence. This insight provides a new opportunity to design high-performance NLO materials using metal phosphates

Two Metal Phosphate Nonlinear Optical Materials Simultaneously Exhibiting Ultraviolet Transparence and a Large Birefringence

For phosphate nonlinear optical (NLO) materials, how to improve their small birefringence is confronted with a great contradiction of their weak optical anisotropy of tetrahedral PO4 groups. Herein, by introducing La3+ with a closed-shell structure and Cd2+ with a d10 electronic configuration, two NLO materials with a large birefringence, namely, La­(PO3)3 (0.040@1064 nm) and β-Cd­(PO3)2 (0.059@1064 nm), have been synthesized by a high-temperature solution method. In particular, for β-Cd­(PO3)2, it possesses the largest birefringence among the known deep-ultraviolet (DUV) phosphates to date, which is attributed to cooperative effects of strong covalence of Cd–O groups and P–O pseudolayers similar to those of a plane. Meanwhile, β-Cd­(PO3)2 exhibits the shortest cutoff edge (<190 nm) among the reported Cd-based inorganic compounds and realizes a balance between DUV transparence and a large birefringence. This insight provides a new opportunity to design high-performance NLO materials using metal phosphates

Growth retardation of <i>Cul4b</i> heterozygous mice during embryonic development.

<p>(A) Bodyweights of <i>Cul4b</i> heterozygous mice and littermate wild-type females after birth. Data were presented as mean±SD. N = 8, *: p<0.05; **: p<0.01; ***: p<0.001. (B–E) Representative photographs of <i>Cul4b</i> heterozygous embryos and littermate wild-type controls at 9.5 (B), 10.5 (C), 12.5 (D) and 14.5 (E) dpc. The bar represents 1 mm in (B–C) and 2 mm in (D) and (E), respectively.</p

Distribution of <i>Cul4b</i> genotypes in progeny of <i>Cul4b</i>^+/flox/<i>Cul4b</i>^+/null;<i>EIIa-Cre</i>^+/− females.

<p>Litters were dissected at the times shown and genotyped by PCR as described in Materials and Methods.</p>a<p>ND indicates that the <i>Cul4b</i> genotype could not be determined by PCR.</p

Characterization of X chromosome inactivation by Cul4b expression in heterozygous mice.

<p>(A–C) Percentages of cells positive for Cul4b of <i>Cul4b</i> heterozygous mice and littermate wild-type female controls at 4 months (A), 3 weeks (B) and newborn (C). More than 2,000 cells of each tissue were scored. Hi, hippocampus; Ki, kidney; Li, liver; Lu, lung. Data were presented as mean±SD. *: p<0.05; **: p<0.01; ***: p<0.001. (D–E) Representative images of liver (D) and hippocampus (E) at 3 weeks stained with an antibody against Cul4b. Sections were counterstained with haematoxylin. Lower panels are the higher magnification of the upper panels. (F) Immunohistochemistry of paraffin sections of <i>Cul4b</i> heterozygous embryos at 7.5 dpc with an anti-Cul4b antibody. Embryos at 7.5 dpc were paraffin embedded and cross sectioned together with their surrounding deciduas. Middle and right panels are the higher magnification of the left panel.</p

Morphology and histology of placentas of wild-type, <i>Cul4b</i> heterozygous and absorbed embryos at 14.5 dpc.

<p>(A) Representative photographs of placentas of wild-type, <i>Cul4b</i> heterozygous and absorbed embryos at 14.5 dpc. (B) H&E staining of radial sections of placentas. sp, spongiotrophoblast layer; la, labyrinthine layer. Lower panels are the higher magnification of the upper panels. (C) Immunohistochemisty of radial sections of placentas with an antibody to PECAM, an angiogenesis marker. Middle panels are the higher magnification of the upper panels, and lower panels are the higher magnification of the middle panels.</p