Simulation of Multipass Hot Rolling for 7050 Aluminum Alloys

To determine the relations between rolling passes, mechanical behaviours and microstructure evolution of AA7050 aluminum alloys, finite element modeling of a multipass hot rolling process is developed and employed to investigate thermo-mechanical evolution during this processing. Through parametric studies, the distribution of local strain and temperature across thickness during the hot rolling process are numerically determined. These results are used to determine the subgrain size and thus the microstructure evolution during the hot rolling process are estimated

sj-pdf-1-het-10.1177_09603271211065972 – Supplemental Material for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis

Supplemental Material, sj-pdf-1-het-10.1177_09603271211065972 for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis by Qing Chang, Jun Wu, Yang An, Haiyan Liu and Yang Sun in Human & Experimental Toxicology</p

sj-xlsx-1-het-10.1177_09603271211065972 – Supplemental Material for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis

Supplemental Material, sj-xlsx-1-het-10.1177_09603271211065972 for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis by Qing Chang, Jun Wu, Yang An, Haiyan Liu and Yang Sun in Human & Experimental Toxicology</p

Representative results of qPCR disassociation curves during screening.

<p>This figure was snipped from our results obtained using ABI qPCR analysis software SDS 2.4 standalone, and shows representative results of disassociation curves generated from the first step of 3S3DBC DNA screening. Positive DNA template (10 ng of <i>Dugesia japonica</i> genomic DNA) was used to detect the qPCR specificity of the respective primer set of DjPiwiB (a) and DJF-033N19 (b). Each primer set could also generate the specific qPCR product after qPCR in a real library super pool (c and d). No nonspecific amplification was detected in the negative super pool or in the H₂O negative template control, using either DjPiwiB or DJF-033N19 multiplex primer sets (e and f). The expected qPCR disassociation curve (double peak shows two qPCR products) was detected in the positive library super pool using DjPiwiB and DJF-033N19 multiplex primer sets (g), and the dissociation temperature values of the two peaks corresponded to the same values obtained in the positive template control and positive super pool results. All figures from (a) to (g) were merged to produce (h).</p

Iron-Catalyzed α‑Arylation of Deoxybenzoins with Arenes through an Oxidative Dehydrogenative Approach

A novel α-arylation of deoxybenzoins with non-prefunctionalized arenes is developed through an iron-catalyzed oxidative dehydrogenative approach. The reaction shows broad substrate scope and functional group tolerance and thus provides efficient access to synthetically useful 1,2,2-triarylethanones. A reasonable mechanism is also proposed

Lats2 inhibits Wnt signaling.

<p>(A) The protein levels of p-DVL2, β-catenin and Wnt signaling targets decrease during adipocyte differentiation. Total cell lysates were prepared from differentiating 3T3L1 cells. (B) Lats2-mediated decrease in β-catenin level. Western blot shows that the levels of DVL2 phosphorylation and β-catenin protein are both reduced by Lats2. (C) Lats2 suppresses the Wnt3a-induced activity of the TOPflash reporter. TOPflash and pRL-TK plasmids were co-transfected into Lats2-transfected cells and the two control (Vector and Control) cells, which were then treated with or without Wnt3a (50 ng/ml). The pRL-TK plasmid (<i>pRenilla</i>) was used as an internal control. The FOPflash assay was used as a negative control. NM, normal medium. (D) and (E) Lats2 inhibits Wnt target gene expression. Quantitative RT-PCR results from Lats2-transfected cells indicate that Pref-1, LEF1, cyclin D1, and c-Myc mRNA levels are reduced by Lats2. The data shown are the means+S.D. of three independent experiments. Western blot shows that the protein levels of cyclin D1, c-Myc, LEF1 and Axin are also reduced by Lats2. In (C) and (D), <i>P</i>-values were calculated using the Student’s t-test (*, <i>P</i><0.05; **, <i>P</i><0.01).</p

Iron-Catalyzed α‑Arylation of Deoxybenzoins with Arenes through an Oxidative Dehydrogenative Approach

A novel α-arylation of deoxybenzoins with non-prefunctionalized arenes is developed through an iron-catalyzed oxidative dehydrogenative approach. The reaction shows broad substrate scope and functional group tolerance and thus provides efficient access to synthetically useful 1,2,2-triarylethanones. A reasonable mechanism is also proposed

Iron-Catalyzed α‑Arylation of Deoxybenzoins with Arenes through an Oxidative Dehydrogenative Approach

A novel α-arylation of deoxybenzoins with non-prefunctionalized arenes is developed through an iron-catalyzed oxidative dehydrogenative approach. The reaction shows broad substrate scope and functional group tolerance and thus provides efficient access to synthetically useful 1,2,2-triarylethanones. A reasonable mechanism is also proposed

A Colony Multiplex Quantitative PCR-Based 3S3DBC Method and Variations of It for Screening DNA Libraries

<div><p>A DNA library is a collection of DNA fragments cloned into vectors and stored individually in host cells, and is a valuable resource for molecular cloning, gene physical mapping, and genome sequencing projects. To take the best advantage of a DNA library, a good screening method is needed. After describing pooling strategies and issues that should be considered in DNA library screening, here we report an efficient colony multiplex quantitative PCR-based 3-step, 3-dimension, and binary-code (3S3DBC) method we used to screen genes from a planarian genomic DNA fosmid library. This method requires only 3 rounds of PCR reactions and only around 6 hours to distinguish one or more desired clones from a large DNA library. According to the particular situations in different research labs, this method can be further modified and simplified to suit their requirements.</p></div

Iron-Catalyzed α‑Arylation of Deoxybenzoins with Arenes through an Oxidative Dehydrogenative Approach

A novel α-arylation of deoxybenzoins with non-prefunctionalized arenes is developed through an iron-catalyzed oxidative dehydrogenative approach. The reaction shows broad substrate scope and functional group tolerance and thus provides efficient access to synthetically useful 1,2,2-triarylethanones. A reasonable mechanism is also proposed