590 research outputs found

    THE APPLICATION MODE OF ATHLETES IN THE CALCULATION OF CLOUD MOTION CAPTURE TECHNOLOGY IN FOOTBALL

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    ABSTRACT Motion capture is a common problem in sports. It is mainly used to measure and capture running distance in football matches. Use of cloud computing motion capture algorithm in football running distance test, for lack of cloud computing algorithm using motion capture in the application. Data are obtained by experiment to verify the effectiveness and feasibility of the improved cloud computing motion capture algorithm in running distance in football. The final conclusion is that, compared with the original cloud computing motion capture algorithm, the optimized cloud computing motion capture algorithm can significantly reduce the number of cycles in the test of football running distance.</div

    Presentation_1_Population Genomics and Haplotype Analysis in Bread Wheat Identify a Gene Regulating Glume Pubescence.PPTX

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    Glume hairiness or pubescence is an important morphological trait with high heritability to distinguish/characterize wheat and is related to the resistance to biotic and abiotic stresses. Hg1 (formerly named Hg) on chromosome arm 1AS controlled glume hairiness in wheat. Its genetic analysis and mapping have been widely studied, yet more useful and accurate information for fine mapping of Hg1 and identification of its candidate gene is lacking. The cloning of this gene has not yet been reported for the large complex wheat genome. Here, we performed a GWAS between SNP markers and glume pubescence (Gp) in a wheat population with 352 lines and further demonstrated the gene expression and haplotype analysis approach for isolating the Hg1 gene. One gene, TraesCSU02G143200 (TaELD1-1A), encoding glycosyltransferase-like ELD1/KOBITO 1, was identified as the most promising candidate gene of Hg1. The gene annotation, expression pattern, function SNP variation, haplotype analysis, and co-expression analysis in floral organ (spike) development indicated that it is likely to be involved in the regulation of glume pubescence. Our study demonstrates the importance of high-quality reference genomes and annotation information, as well as bioinformatics analysis, for gene cloning in wheat.</p

    Presentation_2_Population Genomics and Haplotype Analysis in Bread Wheat Identify a Gene Regulating Glume Pubescence.PPTX

    No full text
    Glume hairiness or pubescence is an important morphological trait with high heritability to distinguish/characterize wheat and is related to the resistance to biotic and abiotic stresses. Hg1 (formerly named Hg) on chromosome arm 1AS controlled glume hairiness in wheat. Its genetic analysis and mapping have been widely studied, yet more useful and accurate information for fine mapping of Hg1 and identification of its candidate gene is lacking. The cloning of this gene has not yet been reported for the large complex wheat genome. Here, we performed a GWAS between SNP markers and glume pubescence (Gp) in a wheat population with 352 lines and further demonstrated the gene expression and haplotype analysis approach for isolating the Hg1 gene. One gene, TraesCSU02G143200 (TaELD1-1A), encoding glycosyltransferase-like ELD1/KOBITO 1, was identified as the most promising candidate gene of Hg1. The gene annotation, expression pattern, function SNP variation, haplotype analysis, and co-expression analysis in floral organ (spike) development indicated that it is likely to be involved in the regulation of glume pubescence. Our study demonstrates the importance of high-quality reference genomes and annotation information, as well as bioinformatics analysis, for gene cloning in wheat.</p

    Data_Sheet_1_Population Genomics and Haplotype Analysis in Bread Wheat Identify a Gene Regulating Glume Pubescence.XLSX

    No full text
    Glume hairiness or pubescence is an important morphological trait with high heritability to distinguish/characterize wheat and is related to the resistance to biotic and abiotic stresses. Hg1 (formerly named Hg) on chromosome arm 1AS controlled glume hairiness in wheat. Its genetic analysis and mapping have been widely studied, yet more useful and accurate information for fine mapping of Hg1 and identification of its candidate gene is lacking. The cloning of this gene has not yet been reported for the large complex wheat genome. Here, we performed a GWAS between SNP markers and glume pubescence (Gp) in a wheat population with 352 lines and further demonstrated the gene expression and haplotype analysis approach for isolating the Hg1 gene. One gene, TraesCSU02G143200 (TaELD1-1A), encoding glycosyltransferase-like ELD1/KOBITO 1, was identified as the most promising candidate gene of Hg1. The gene annotation, expression pattern, function SNP variation, haplotype analysis, and co-expression analysis in floral organ (spike) development indicated that it is likely to be involved in the regulation of glume pubescence. Our study demonstrates the importance of high-quality reference genomes and annotation information, as well as bioinformatics analysis, for gene cloning in wheat.</p

    sj-pdf-1-chc-10.1177_1367493520933458 – Association of healthy child-rearing practices and children’s receipt of care in patient-centered medical homes

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    Supplemental Material, sj-pdf-1-chc-10.1177_1367493520933458 Association of healthy child-rearing practices and children’s receipt of care in patient-centered medical homes by Victoria L Phillips and Xin Hu in Journal of Child Health Care</p

    sj-pdf-2-chc-10.1177_1367493520933458 – Association of healthy child-rearing practices and children’s receipt of care in patient-centered medical homes

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    Supplemental Material, sj-pdf-2-chc-10.1177_1367493520933458 Association of healthy child-rearing practices and children’s receipt of care in patient-centered medical homes by Victoria L Phillips and Xin Hu in Journal of Child Health Care</p

    In vitro ingestion and inhalation bioaccessibility of soilborne lead, cadmium, arsenic and chromium near a chemical industrial park for health risk assessment

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    Surface soils were collected near a chemical industrial park in the present study and in vitro inhalation and ingestion bioaccessibility of soilborne lead, cadmium, arsenic and chromium was evaluated using various in vitro bioaccessibility procedures including simulated lung fluid (SLF), artificial lysosomal fluid (ALF), modified Gamble solution (MGS), Solubility/Bioaccessibility Research Consortium (SBRC) procedure, and in vitro gastrointestinal (IVG) procedure. The in vitro inhalation and ingestion bioaccessibility of toxic elements showed elemental dependence and differed greatly among SBRC, IVG, SLF, ALF and MGS. The non-carcinogenic and lifetime carcinogenic risks of soilborne toxic elements based on the bioaccessible contents via ingestion and inhalation exposure were within the acceptable level. The present study reveals that the in vitro bioaccessibility of toxic elements was influenced greatly by the current in vitro bioaccessibility procedures, which resulted in great differences on the risk-based assessment via inhalation and oral ingestion exposure.</p

    Targeting Plague Virulence Factors:  A Combined Machine Learning Method and Multiple Conformational Virtual Screening for the Discovery of <i>Yersinia</i> Protein Kinase A Inhibitors

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    Yersinia spp. is currently an antibiotic resistance concern and a re-emerging disease. The essential virulence factor Yersinia protein kinase A (YpkA) contains a Ser/Thr kinase domain whose activity modulates pathogenicity. Here, we present an approach integrating a machine learning method, homology modeling, and multiple conformational high-throughput docking for the discovery of YpkA inhibitors. These first reported inhibitors of YpkA may facilitate studies of the pathogenic mechanism of YpkA and serve as a starting point for development of anti-plague drugs

    Dynamic Assembly by Electrokinetic Microfluidics

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    Dynamic assembly induced by electrokinetic flows was investigated in a two-dimensional tapered microchannel. Vortices formed both at the outlet of the small end and inside the channel. These vortices led to electrolyte deposition on oppositely charged channel surfaces during the two-stage assembly process. The primary assembly occurred in the stagnation region of a double vortex, which was formed by a nonuniform surface ζ-potential distributed along the tapered channel. This was followed by a slower secondary assembly resulting from circulation of the vortex inside the channel. Since electrolyte deposition kept changing the surface charge distribution, the double vortex migrated in the channel. Consequently, the assembly started from the small end and gradually extended toward the large end. Continuous feeding of diluted electrolytes resulted in a uniform assembly on the channel surface, useful for grafting functional molecules and nanoparticles in long micro- and nanoscale channels. When multiple-dosage feeding was applied, an asymmetric assembly occurred, forming a gradient of surface property, which is useful for combinatorial techniques and the formation of multifunctional micro-/nanoconstructs. This new method provides a useful tool to overcome the limitation of the current self-assembly methods

    Interaction of the Disordered Yersinia Effector Protein YopE with Its Cognate Chaperone SycE

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    We describe an efficient approach to model the binding interaction of the disordered effector protein to its cognate chaperone in the type III secretion system (T3SS). Starting from de novo models, we generated ensembles of unfolded conformations of the Yersinia effector YopE using REMD simulations and docked them to the chaperone SycE using a multistep protein docking strategy. The predicted YopE/SycE complex was in good agreement with the experimental structure. The ability of our computational protocol to mimic the structural transition upon chaperone binding opens up the possibility of studying the underlying specificity of chaperone/effector interactions and devising strategies for interfering with T3SS transport
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