Visualization 1: New method to measure liquid diffusivity by analyzing an instantaneous diffusion image

A dynamic process recorded by CCD when the chemical EG diffusing in pure water. Originally published in Optics Express on 07 September 2015 (oe-23-18-23155

Visualization 1: Asymmetric liquid-core cylindrical lens used to measure liquid diffusion coefficient

The dynamic diffusion process for triethylene glycol diffusing in water. Originally published in Applied Optics on 10 March 2016 (ao-55-8-2011

Copper-Catalyzed Enantioselective C–H Arylation between 2‑Arylindoles and Hypervalent Iodine Reagents

The copper-catalyzed enantioselective C–H arylation between 2-arylindoles and hypervalent iodine reagents has been successfully developed, which provides a convenient and economical route to the highly atroposelective synthesis of axially chiral indole derivatives with a 2-aryl structure (up to 99% ee). Density functional theory calculations and wave function analysis show that the key “sandwich” intermediate leads to high enantioselectivity of the reaction

Comparison of real-time resistance measurement and culture identification.

*<p>There is no statistical difference between the two methods (P>0.05).</p

Primers for <i>V. parahaemolyticus.</i>

<p>Primers for <i>V. parahaemolyticus.</i></p

Specificity analysis of the real-time resistance measurement.

<p>A. The real-time resistance curve of <i>V. parahaemolyticus</i> and other interfering bacteria. B. End-point resistance of <i>V. parahaemolyticus</i> and other interfering bacteria after a 60-min LAMP assay. C. Derivative analysis of the real-time resistance measurement.</p

Sensitivity and regression analyses of the real-time resistance measurement.

<p>A. The real-time resistance curve of <i>V. parahaemolyticus</i> in a concentration gradient. B. The derivative analysis of the real-time resistance measurement of <i>V. parahaemolyticus</i>. C. The regression analysis of the real-time resistance measurement of <i>V. parahaemolyticus.</i> Three samples of the same bacterial concentration were measured twice and all values were recorded as mean(n = 6).</p

Scheme of the real-time resistance measurement for <i>V. parahaemolyticus</i>.

<p>Briefly, the lecithin-dependent hemolysin (LDH) gene of <i>V. parahaemolyticus</i> was amplified by LAMP at first. The subsequent two products, DNA and pyrophosphate, both negative ions, were combined with a positive dye (Crystal violet) and positive ions (Mg2+), leading to an increase in the reaction liquid resistance. This resistance was measured in real-time using a electrode, and <i>V. parahaemolyticus</i> concentration was quantitatively detected through a derivative analysis.</p

GLIPR-2 Overexpression in HK-2 Cells Promotes Cell EMT and Migration through ERK1/2 Activation

<div><p>The epithelial-to-mesenchymal transition (EMT) of tubular epithelial cells in the adult kidney is one of the key events in renal interstitial fibrosis. Glioma pathogenesis related-2 (GLIPR-2) has been shown to be up-regulated in proximal tubular cells (PTCs) in the fibrotic kidney. However, the biological function of GLIPR-2 remains unknown. In this study, we found that GLIPR-2 expression is elevated in the kidney tissue samples of patients with diabetic nephropathy (DN). Human proximal renal tubular epithelial cells (HK-2 cells) were transfected with pcDNA3.0-GLIPR-2 and selected with G418. To identify the biological function of GLIPR-2, an epithelial-to-mesenchymal transition (EMT) PCR array analysis was performed, and genes that had statistically significantly altered expression levels with more than a two-fold difference compared with the pcDNA3.0-transfected HK-2 cells were considered. Key elements of the EMT process, such as E-cadherin and vimentin, were transcriptionally activated in the pcDNA3.0-GLIPR-2-transfected sublines. In addition, α-SMA gene expression, which is a marker of myofibroblasts, increased in the pcDNA3.0-GLIPR-2-transfected HK-2 cells. The cell migration assay demonstrated that the transfection of HK-2 with GLIPR-2 promoted cell migration following an EMT. Additionally, consistent with the effects of increased EGFR expression levels, we found that the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) was highly elevated in the pcDNA3.0-GLIPR-2-transfected group. Our study demonstrates that GLIPR-2 overexpression in HK-2 cells can potentiate EMT-like processes in this cell type through the ERK1/2 signaling pathway. GLIPR-2 may be responsible for the development of renal fibrosis by increasing the accumulation of interstitial fibroblasts.</p> </div

Genes that are up- and down-regulated by the overexpression of GLIPR-2.

<p>Genes that are up- and down-regulated by the overexpression of GLIPR-2.</p