Principle of OI array analysis.

<p>Eight probes (four each for sense and antisense strands) associated with every queried site were used. Each probe consisted of a 25-base oligonucleotide, while the 13th base was different among the probes to cover all potential nucleotide mutations.</p

Development of a High-Throughput Resequencing Array for the Detection of Pathogenic Mutations in Osteogenesis Imperfecta

<div><p>Objective</p><p>Osteogenesis imperfecta (OI) is a rare inherited skeletal disease, characterized by bone fragility and low bone density. The mutations in this disorder have been widely reported to be on various exonal hotspots of the candidate genes, including <i>COL1A1</i>, <i>COL1A2</i>, <i>CRTAP</i>, <i>LEPRE1</i>, and <i>FKBP10</i>, thus creating a great demand for precise genetic tests. However, large genome sizes make the process daunting and the analyses, inefficient and expensive. Therefore, we aimed at developing a fast, accurate, efficient, and cheaper sequencing platform for OI diagnosis; and to this end, use of an advanced array-based technique was proposed.</p><p>Method</p><p>A CustomSeq Affymetrix Resequencing Array was established for high-throughput sequencing of five genes simultaneously. Genomic DNA extraction from 13 OI patients and 85 normal controls and amplification using long-range PCR (LR-PCR) were followed by DNA fragmentation and chip hybridization, according to standard Affymetrix protocols. Hybridization signals were determined using GeneChip Sequence Analysis Software (GSEQ). To examine the feasibility, the outcome from new resequencing approach was validated by conventional capillary sequencing method.</p><p>Result</p><p>Overall call rates using resequencing array was 96–98% and the agreement between microarray and capillary sequencing was 99.99%. 11 out of 13 OI patients with pathogenic mutations were successfully detected by the chip analysis without adjustment, and one mutation could also be identified using manual visual inspection.</p><p>Conclusion</p><p>A high-throughput resequencing array was developed that detects the disease-associated mutations in OI, providing a potential tool to facilitate large-scale genetic screening for OI patients. Through this method, a novel mutation was also found.</p></div

It is a reverse sequencing for the insertion GAT by direct sequencing.

<p>It is a reverse sequencing for the insertion GAT by direct sequencing.</p

The insertion GAT verified by direct sequencing in patient 8^#.

<p>It is a reverse sequencing, and the frame shifted to move right with the duplication GAT.</p

Additional file 1 of Perceptions and attitudes of dental practitioners towards impacts of Covid 19 pandemic on clinical dentistry: a cross-sectional study

Additional file 1: Supplementary Table 1. Bivariate analysis results

Mutations identified in OI patients.

<p>* new mutation found in the present study</p><p>Mutations identified in OI patients.</p

Variants identification, selection, filtering.

<p>(A)The name of the fragment, the position of the altered nucleotides and the reference nucleotides are signed on the left side. The altered nucleotides of the samples are itemized. The sign N (blue) corresponds to the intensity of the signal which did not allow for a specific base call. The signs y, r, k, etc. (orange) indicate a nucleotide change in the heterozygous state. Signs a, t, c or g (green) denote a nucleotide change to homozygous A, T, C or G. (B) Sequence output files of samples. Part of the sequence containing nucleotide alterations is shown. Reference sequence and positions of nucleotides are shown in red at the top. The signs are identical to those in image (A).</p

Facile Fabrication of Superhydrophobic Sponge with Selective Absorption and Collection of Oil from Water

A simple vapor-phase deposition process has been developed to fabricate a superhydrophobic and superoleophilic sponge using ordinary commercial polyurethane sponges. The simultaneous properties of superhydrophobicity and superoleophilicity enable the sponge to float on the water surface and selectively absorb oil from water. Its uptake capacities of different oils (motor oil, lubricating oil, pump oil, silicone oil, and soybean oil) in the oil–water mixtures were all above 20 g/g. The absorbed oil could be collected by squeezing the sponge, and the recovered sponge could be reused in oil–water separation for many cycles while still maintaining a high capacity. This is helpful for realizing the proper disposal of the oil and avoiding secondary pollution. A similar experiment was performed using the as-prepared sponge to remove petroleum from contaminated water. The results suggest that our material might find practical applications in the cleanup of oil spills and the removal of organic pollutants from water surfaces

Facile Fabrication of Superhydrophobic Sponge with Selective Absorption and Collection of Oil from Water

A simple vapor-phase deposition process has been developed to fabricate a superhydrophobic and superoleophilic sponge using ordinary commercial polyurethane sponges. The simultaneous properties of superhydrophobicity and superoleophilicity enable the sponge to float on the water surface and selectively absorb oil from water. Its uptake capacities of different oils (motor oil, lubricating oil, pump oil, silicone oil, and soybean oil) in the oil–water mixtures were all above 20 g/g. The absorbed oil could be collected by squeezing the sponge, and the recovered sponge could be reused in oil–water separation for many cycles while still maintaining a high capacity. This is helpful for realizing the proper disposal of the oil and avoiding secondary pollution. A similar experiment was performed using the as-prepared sponge to remove petroleum from contaminated water. The results suggest that our material might find practical applications in the cleanup of oil spills and the removal of organic pollutants from water surfaces

A Highly Selective Fluorescent Sensor for Distinguishing Cadmium from Zinc Ions Based on a Quinoline Platform

A fluorescent sensor, <i>N</i>-(quinolin-8-yl)-2-(quinolin-8-yloxy)­acetamide (<b>HL</b>), based on 8-aminoquinoline and 8-hydroxyquinoline platforms has been synthesized. This sensor displays high selectivity and sensitive fluorescence enhancement to Cd²⁺ in ethanol. Moreover, sensor <b>HL</b> can distinguish Cd²⁺ from Zn²⁺ via two different sensing mechanisms (photoinduced electron transfer for Cd²⁺; internal charge transfer for Zn²⁺). The composition of the complex Cd²⁺/<b>HL</b> or Zn²⁺/<b>L</b>^– has been found to be 1:1, based on the fluorescence/absorption titration and further confirmed by X-ray crystallography