MOESM1 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 1. Additional methods and results

MOESM4 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 4. Differentially expressed genes for M2 vs SPT15

MOESM3 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 3. RPKM values and fold changes of genes in the central carbon metabolic network

MOESM2 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 2. Logistic fitting results

MOESM5 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 5. GO enrichment of differentially expressed genes for M2 vs SPT15

MOESM6 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 6. KEGG enrichment of differentially expressed genes for M2 vs SPT15

L'Auto-vélo : automobilisme, cyclisme, athlétisme, yachting, aérostation, escrime, hippisme / dir. Henri Desgranges

13 janvier 19371937/01/13 (A38,N13176)

Revealing Potential Genes Affecting Flocculation and/or Viability of Saccharomyces pastorianus by Comparative Genomic Analysis

Yeast flocculation and viability are critical factors in beer production. Adequate flocculation of yeast at the end of fermentation helps to reduce off-flavors and cell separation, while high viability is beneficial for yeast reuse. In this study, we used comparative genomics to analyze the genome information on Saccharomyces pastorianus W01, and its spontaneous mutant W02 with appropriate weakened flocculation ability (better off-flavor reduction performance) and unwanted decreased viability, to investigate the effect of different gene expressions on yeast flocculation or/and viability. Our results indicate that knockout of CNE1, CIN5, SIN3, HP-3, YPR170W-B, and SCEPF1_0274000100 and overexpression of CNE1 and ALD2 significantly decreased the flocculation ability of W01, while knockout of EPL1 increased the flocculation ability of W01. Meanwhile, knockout of CIN5, YPR170W-B, OST5, SFT1, SCEPF1_0274000100, and EPL1 and overexpression of SWC3, ALD2, and HP-2 decreased the viability of W01. CIN5, EPL1, SCEPF1_0274000100, ALD2, and YPR170W-B have all been shown to affect yeast flocculation ability and viability