sj-docx-1-mrx-10.1177_01979183231162623 - Supplemental material for Expected Occupational Contexts and Postgraduate Pursuit among Skilled Immigrants and Natives in the United States

Supplemental material, sj-docx-1-mrx-10.1177_01979183231162623 for Expected Occupational Contexts and Postgraduate Pursuit among Skilled Immigrants and Natives in the United States by Xiao Yu in International Migration Review</p

Nanoparticle-Based Biocompatible and Targeted Drug Delivery: Characterization and in Vitro Studies

Paclitaxel nanoparticles (PAX NPs) prepared with the size of 110 ± 10 nm and ζ potential of −40 ± 3 mV were encapsulated in synthetic/biomacromolecule shell chitosan, dextran-sulfate using a layer-by-layer self-assembly technique. Zeta potential measurements, analysis of X-ray photoelectron spectroscopy, and scanning electron microscopy confirmed the successful adsorption of each layer. Surface modifications of these core–shell NPs were performed by covalently conjugating with poly(ethylene glycol) (H2N-PEG-carboxymethyl, Mw 3400) and fluorescence labeled wheat germ agglutinin (F-WGA) to build a biocompatible and targeted drug delivery system. 32% of PAX was released from four bilayers of biomacromolecule assembled NPs within 8 h as compared with >85% of the drug released from the bare NPs. Moreover, high cell viability with PEG conjugation and high binding capacity of WGA-modified NPs with Caco-2 cells were observed. This biocompatible and targeted NP-based drug delivery system, therefore, may be considered as a potential candidate for the treatment of colonic cancer and other diseases

Photocatalytic Metal–Organic Frameworks for Selective 2,2,2-Trifluoroethylation of Styrenes

Synthesis of CF₃-containing compounds is of great interest because of their broad use in the pharmaceutical and agrochemical industries. Herein, selective 2,2,2-trifluoroethylation of styrenes was catalyzed by Zr­(IV)-based MOFs bearing visible-light photocatalysts in the form of Ir­(III) polypyridyl complexes. When compared to the homogeneous Ir­(III) catalyst, the MOF-based catalyst suppressed the dimerization of benzyl radicals, thus enhancing the selectivity of the desired hydroxy­trifluoroethyl compounds

The complete chloroplast genome of <i>Cynoglossum amabile</i> Stapf & J. R. Drumm., 1906 (Boraginaceae), a traditional Chinese herbal medicine

Cynoglossum amabile Stapf & J. R. Drumm., 1906 is a traditional Chinese herbal medicine from southwest China. To better determine its phylogenetic relatedness to other Boraginaceae species, the chloroplast (cp) genome of C. amabile was sequenced. The complete cp genome of C. amabile is 151,532 bp in length, containing a small single-copy (SSC) region with a length of 17,366 bp, a large single-copy (LSC) region with a length of 82,902 bp, and a pair of inverted repeats (IRs) regions each with a length of 25,632 bp. The overall GC content of the cp genome is 37.4%. The maximum-likelihood phylogenetic tree showed that Bothriospermum zeylanicum (J. Jacq.) Druce, 1917 was closely related to C. amabile.</p

The complete chloroplast genome of <i>Crataegus scabrifolia</i> (Franch.) Rehd (Rosaceae), a medicinal and edible plant in Southwest China

Crataegus scabrifolia (Franch.) Rehd is a medicinal and edible plant in Southwest China. The chloroplast genome of C. scabrifolia was analyzed by high-throughput sequencing technology, and its genetic relationship to related species was discussed. The chloroplast genome is 159,637 bp long, with two inverted repeat (IR) regions (26,384 bp each) that separate a large single-copy (LSC) region (87,730 bp) and a small single-copy (SSC) region (19,139 bp). A total of 127 genes were annotated, including 83 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. The phylogenetic tree shows that C. hupehensis is closely related to C. scabrifolia with strong bootstrap support.</p

The complete chloroplast genome of <i>Elsholtzia fruticosa</i> (D. Don) Rehd. (Labiatae), an ornamental plant with high medicinal value

Elsholtzia fruticosa is an ornamental plant with high medicinal value. In this study, we sequenced and analyzed the complete chloroplast (cp) genome of the species. The complete cp sequence is 151,550 bp, including the large single-copy (LSC) region of 82,778 bp, the small single-copy (SSC) region of 17,492 bp, and a pair of invert repeats (IRs) regions of 25,640 bp. It encodes 132 unique genes in total, including 87 protein-coding genes, 37 transfer RNA genes (tRNAs), and eight ribosomal RNA genes (rRNAs). The comparative analysis of complete cp genomes showed that the genomic structure and gene order of E. fruticosa cps were conserved. The sequences of rps15, rps19, ycf1, ycf3, ycf15, psbL, psaI, trnG-UCC, trnS-GCU, trnR-UCU, trnL-UAG, trnP-UG, and trnL-UAA serve as hotspots for developing the DNA barcoding of Elsholtzia species. There are 49 SSR loci in the cp genome of E. fruticosa, among which the repeat numbers of mononucleotide, dinucleotide, trinucleotide, tetranucleotide, and pentanucleotides SSR are 37, 9, 3, 0, and 0, respectively. A total of 50 repeats were detected, including 15 forward repeats, seven reverse repeats, 26 palindromic repeats, and two complementary repeats. Phylogenetic analysis based on the complete cp genome and protein-coding DNA sequences of 26 plants indicates that E. fruticosa has a dose relationship with E. splendens and E. byeonsanensis.</p

Additional file 1 of High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China

Supplementary material 1

Efficient Electrochemical Nitrate Removal by Ordered Ultrasmall Intermetallic AuCu₃ via Enhancing Nitrate Adsorption

Developing a high-performance electrocatalyst for synthesizing ammonia from nitrate represents a promising solution for addressing wastewater pollution and achieving sustainable ammonia production. However, it remains a formidable challenge. Herein, an intermetallic AuCu3 electrocatalyst with high-density active sites is designed and prepared for an efficient nitrate electroreduction to generate ammonia. Remarkably, the Faraday efficiency and yield rate of ammonia at −0.9 V are 97.6% and 75.9 mg h–1 cm–2, respectively. More importantly, after 10 cycles of testing, the removal rate of nitrate can still reach 95.2%. Electrochemical in situ Fourier transform infrared analysis indicates that AuCu3 IM can promote the adsorption of nitrate and enhance ammonia production from nitrate. *NH3, *NO, and *NO2 have been proven to be active intermediates. Theoretical and experimental studies show that the Au site can provide a large amount of *H for nitrate reduction, and the Cu site is conducive to the reduction of nitrate to produce nitrogen-containing products. Meanwhile, AuCu3 intermetallic compounds (AuCu3 IM) can inhibit the dimerization of *H. The power density and ammonia yield of the assembled Zn-nitrate battery reached 2.17 mW cm–2 and 71.2 mg h–1 cm–2, respectively

Transcriptional factor 3 binds to sirtuin 1 to activate the Wnt/β-catenin signaling in cervical cancer

Transcriptional factor 3 (TCF3, also termed E2A), first reported to exert crucial functions during lymphocyte development, has been revealed to participate in the pathogenesis of human cancers. The aim of this work was to investigate the function of TCF3 in cervical cancer (CC) and the molecular interactions. The bioinformatics prediction suggested that TCF3 was highly expressed in CC and linked to poor prognosis. Increased TCF3 expression was identified in CC cell lines, and its downregulation reduced proliferation and migration of CC cells in vitro as well as growth of xenograft tumors in vivo. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the TCF-3-related genes and genes showed differential expression between CC and normal tissues were mainly enriched in the Wnt/β-catenin pathway. TCF3 bound to sirtuin 1 (SIRT1) promoter for transcriptional activation, and SIRT1 promoted deacetylation and nuclear translocation of β-catenin in CC. SIRT1 overexpression blocked the role of TCF3 silencing and restored cell proliferation in vitro and tumor growth in vivo. Treatment with XAV-939, a β-catenin inhibitor, significantly suppressed the cell proliferation and tumor growth induced by SIRT1 overexpression. In conclusion, this study demonstrates that TCF3 augments progression of CC by activating SIRT1-mediated β-catenin signaling.</p

Experimental investigation of air pressure affecting filtration performance of fibrous filter sheet

Understanding the effect of air pressure on their filtration performance is important for assessing the effectiveness of fibrous filters under different practical circumstances. The effectiveness of three classes of air filter sheets were investigated in laboratory-based measurements at a wide range of air pressures (60–130 KPa). The filtration efficiency was found most sensitive to the air pressure change at smaller particle sizes. As the air pressure increased from 60 to 130 KPa, significant decrease in filtration efficiency (up to 15%) and increase in pressure drop (up to 90 Pa) were observed. The filtration efficiency of the filter sheet with largest fiber diameter and smallest solid volume fraction was affected most, while the pressure drop of the filter sheet with smallest fiber diameter and largest solid volume fraction was affected most. The effect of air pressure on the filtration efficiency was slightly larger at greater filter face air velocity. However, the effect of air pressure on the pressure drop was negligible. The filtration efficiency and pressure drop were explicitly expressed as functions of the air pressure. Two coefficients were empirically derived and successfully accounted for the effects of air pressure on filtration efficiency and pressure drop.</p