25 research outputs found

    Increased lncRNA-ROR in hypertrophic cardiomyocytes cultured in vitro.

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    <p>(A) Immunocytochemical staining with anti-α-actinin shows the enlargement of cultured cardiomyocytes after PE treatment. The mRNA (B) and protein (C) expression levels of ANP and BNP were increased in PE-induced hypertrophic cardiomyocytes as determined by quantitative RT-PCR and western blot, respectively. (D) Relative mRNA expression of lncRNA-ROR in the cultured cardiomyocytes after PE treatment. * p<0.05, ** p<0.01 as compared with the control.</p

    Increased lncRNA-ROR in hypertrophic heart in vivo.

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    <p>(A) Relative mRNA expression levels of ANP and BNP in sham group (Sham) and TAC group (TAC) determined by quantitative RT-PCR. (B) Western blot showing the protein expression of ANP and BNP in control and hypertrophic heart. GAPDH serves as loading control. (C) Relative mRNA expression of lncRNA-ROR in the mouse hearts of Sham group and TAC group.</p

    Knockdown of lncRNA-ROR reversed hypertrophic responses of cardiomyocytes.

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    <p>(A) After the transfection of ROR-specific siRNA, the mRNA expression of lncRNA-ROR was dramatically decreased compared with the control siRNA-transfected cells. (B) The enlarged cardiomyocytes induced by PE treatment was attenuated by the transfection of lncRNA-ROR. The mRNA (C) and protein (D) expression levels of ANP and BNP were decreased after the knockdown of lncRNA-ROR. * p<0.05, ** p<0.01 compared with control siRNA-treated cells.</p

    Overexpression of miR-133 attenuated hypertrophic response.

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    <p>(A) RT-PCR showed that the expression of lncRNA-ROR was decreased by overexpression of miR-133 in a concentration-dependent manner. Relative expression levels of mRNA (B) or protein (C) of ANP and BNP were decreased by overexpression of miR-133. NC represents negative control. * p<0.05, ** p<0.01.</p

    MicroRNA 133 negatively correlated with lncRNA-ROR.

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    <p>(A) Relative mRNA expression levels of miR-21, miR-208, and miR-449 were increased in cultured cardiomyocytes after PE treatment. (B) Relative mRNA expression levels of miR-1, miR-133, and miR-30 were decreased in cultured cardiomyocytes after PE treatment. Plotting the expression of miR-133 (C) or miR-208 (D) against that of lncRNA-ROR in the heart of mice model of cardiac hypertrophy showed that there was no correlation between miR-208 and lncRNA-ROR, however, miR-133 negatively correlated with lncRNA-ROR. * p<0.05, ** p<0.01 compared between control and PE-treated cells.</p

    Preparation and Evaluation of Self-Assembled Porous Microspheres–Fibers for Removal of Bisphenol A from Aqueous Solution

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    A novel design of PP-<i>g</i>-DMAEMA/PM composite fiber as an efficient adsorbent was demonstrated by combining graft polymerization of dimethylaminoethyl methacrylate (DMAEMA) with self-assembled modification of porous microspheres (PMs) on the surface of polypropylene (PP) fiber. The structure and composition of the adsorbent was characterized by BET, XPS, FTIR, DSC, FESEM, and water angle. The kinetics and isotherm data indicated that the adsorption of bisphenol A (BPA) could be well-fitted by a pseudo-second-order kinetic model and the Langmuir isotherm, respectively. The thermodynamic studies indicated that the adsorption reaction was a spontaneous and exothermic process. Because of the π–π interactions and hydrogen bonds between BPA and PP-<i>g</i>-DMAEMA/PM, the resulting fiber obtained a higher adsorption amount (44.43 mg/g) of BPA. The presence of NaCl in the solution could facilitate the adsorption process, whereas the strong acid or strong alkali conditions and higher temperature of the solution were unfavorable. Besides, the obtained fiber reusability without obvious deterioration in performance was demonstrated by at least seven repeated cycles

    Adsorption of Bisphenol A Based on Synergy between Hydrogen Bonding and Hydrophobic Interaction

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    The study mainly investigated the synergetic adsorption of hydrogen bonding and hydrophobic interaction. To simplify the adsorption driving forces and binding sites, the hydrophilic and hydrophobic microdomain was introduced onto polypropylene (PP) nonwoven. The amphiphilic structure was constructed for the adsorption of bisphenol A (BPA). A solvent shielding experiment was conducted to calculate the contributions of diverse interactions. Also, a specific structure without hydrophilic microdomain was constructed as comparison to determine the adsorption rate and quantify the diffusion behaviors. On the basis of double-exponential model, the adsorption process can be distinctly divided into three stages, namely film diffusion stage, intralayer diffusion stage, and dynamic equilibrium stage. The adsorption rate was dramatically improved due to the influence of hydrophilic microdomain and participation of hydrogen bonding adsorption. Discussions on adsorption priority were also proposed. The results of surface energy heterogeneity revealed that the hydrophilic microdomain or the hydrogen bonding site was occupied preferentially

    Fabrication of Hydrophilic and Hydrophobic Sites on Polypropylene Nonwoven for Oil Spill Cleanup: Two Dilemmas Affecting Oil Sorption

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    This article mainly deals with the following dilemmas, which affect oil sorption and sorbent preparation: (1) hydrophobization could facilitate oil sorption but has adverse impacts on emulsion sorption; (2) micropores of conventional oil sorbent do not exhibit effective emulsion sorption. To solve the above contradictions, hydrophilic and hydrophobic sites were fabricated onto polypropylene (PP) nonwoven through electron beam radiation and subsequent ring-opening reaction. Further, a similar structure without a hydrophilic site was constructed as comparison to verify the dilemmas. An oil sorption and emulsion adsorption experiment revealed that the PP nonwoven with specific hydrophilic and hydrophobic sites is more suitable for oil cleanup. The hydrophobic site preserved its hydrophobicity and sorption capacity, and the hydrophilic site on PP surface effectively increased the affinity between the hydrophilic interface of emulsion and sorbent. The overlapped and intertwined structures could provide spaces large enough to accommodate oil and emulsion. In addition, the oil and emulsion sorption behaviors were systematically analyzed. The PP nonwoven fabricated in this study may find practical application in the cleanup of oil spills and the removal of organic pollutants from water surfaces

    Mouse primary acinar cells express Il-10.

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    <p>(<b>A</b>)<b>.</b> Morphology of primary acinar cells isolated from mouse pancreas. Magnification, 400×; red scale, 20 µm. (<b>B</b>) RT-PCR showed the expression of <i>Il-10</i> in mice acinar cells isolated from four different mice. Real time PCR showed the expression of <i>Il-10</i> in mice primary acinar cells treated with (<b>C</b>) different concentration of cerulein (0∼20 µM) and (<b>D</b>) different concentration of recombinant Shh (0∼0.8 µg/ml). <i>Gapdh</i> was used as an endogenous control (n = 4).</p

    Manipulation of LncRNA UCA1 expression changes breast cancer cell drug resistance.

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    <p>(A) qRT-PCR analysis shows the lncRNA UCA1 expression in the tamoxifen-resistant and their parental breast cancer cells. Normal, the parental MCF-7 or T47D cells; TAM-R, tamoxifen resistant MCF-7 or T47D cells. Overexpression of lncRNA UCA1 using lentivirus (Lv-empty vector or Lv-UCA1) in MCF-7 cells (B) or T47D cells (C) promotes the proliferation of breast cancer cells detected with MTT assay. Knockdown of lncRNA UCA1 using small interfering RNA targeting UCA1 (si-UCA1) decreases the proliferation of tamoxifen-resistant MCF-7 (D) or T47D (E) breast cancer cells, respectively. All data represents at least three independent experiments. *, <i>P</i><0.05; **, <i>P</i><0.01.</p
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