The practice of a mixed methods research strategy: personal, professional and project considerations

Effect of DE value on starch solubility of kudzu root starch

MOESM1 of Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli

Additional file 1: Figure S1. Gene function annotations of R12 genome in GO, COG and KEGG databases. Figure S2. The antiSMASH analysis results of R12 genome. Figure S3. The orientation and distribution of the carotenoid biosynthetic genes from the R1 and R12. Table S1. Bioinformatic analysis of key enzymes of carotenoid biosynthesis in R12. Table S2. Percentages of sequence identity of proteins in carotenoid biosynthesis between D. wulumuqiensis R12 and other Deinococcus spp

Preoperative socieodemographic, clinical, CLVQOL and self-rated satisfaction data, and primary surgery data of 140 eligible RRD patients^*.

<p>*CLVQOL: Chinese-version Low Vision Quality of Life Questionnaire, RRD: rhegmatogenous retinal detachment, BCVA: best corrected visual acuity, SD: standard deviation.</p

Preoperative and postoperative total CLVQOL composite scores [median (range)] in 32 macula-on and 60 macula-off RRD patients^*.

<p>*CLVQOL: Chinese-version Low Vision Quality of Life Questionnaire, RRD: rhegmatogenous retinal detachment.</p

Additional file 1: Figure S1. of Optimization of bioconversion process for trehalose production from enzymatic hydrolysis of kudzu root starch using a visualization method

Principles of visualization method

Preoperative and postoperative logMAR BCVA, CLVQOL scores and self-rated satisfaction degrees in 92 RRD patients^*.

<p>*CLVQOL: Chinese-version Low Vision Quality of Life Questionnaire, RRD: rhegmatogenous retinal detachment, BCVA: best corrected visual acuity, SD: standard deviation.</p

Mean number of hydrogen bonds between different molecules in different solutions ^a.

<p>Mean number of hydrogen bonds between different molecules in different solutions <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152275#t002fn001" target="_blank">^a</a>.</p

Interfacial Bioorthogonal Cross-Linking

Described herein is interfacial bioorthogonal cross-linking, the use of bioorthogonal chemistry to create and pattern biomaterials through diffusion-controlled gelation at the liquid-gel interface. The basis is a rapid (<i>k</i>₂ 284000 M^–1 s^–1) reaction between strained <i>trans</i>-cyclooctene (TCO) and tetrazine (Tz) derivatives. Syringe delivery of Tz-functionalized hyaluronic acid (HA-Tz) to a bath of bis-TCO cross-linker instantly creates microspheres with a cross-linked shell through which bis-TCO diffuses freely to introduce further cross-linking at the interface. Tags can be introduced with 3D resolution without external triggers or templates. Water-filled hydrogel channels were prepared by simply reversing the order of addition. Prostate cancer cells encapsulated in the microspheres have 99% viability, proliferate readily, and form aggregated clusters. This process is projected to be useful in the fabrication of cell-instructive matrices for in vitro tissue models

RMSF in simulations of CRL.

<p>The data were indicated the flexibility of CRL calculated from the last 6 ns of simulations in pure water (black), in binary DMF solution (blue), and in ternary DMF/trehalose solution (red).</p

Radial distribution functions.

<p>RDFs for water oxygen, DMF carbon, and trehalose hydroxyl oxygen around the center of mass (COM) of the protein as a function of the distance <i>r</i> from COM of the protein in binary DMF solution (A) and a ternary DMF/trehalose solution (B) over the last 6 ns of the simulation.</p