Additional file 8 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 8: Fig. S8. Comparison of WM score and EMT score on hazard ratio, C-index, and 1-year, 3-year and 5-year AUC. CI, confidence interval. AUC, area under ROC curve

Additional file 1 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 1: Fig. S1. Enriched pathways of significantly mutated genes evaluated by PathScore

Additional file 3 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 3: Fig. S3. The top 10 significantly enriched BP terms (A) and MF (B) terms of clusters 1 and 2. FDR < 0.05

Additional file 9 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 9: Table S1. Clinical information of TCGA-STAD dataset

Additional file 2 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 2: Fig. S2. IHC results of ADARB1 and RBM15B in gastric cancer tissues. (A-B) IHC of ADARB1 in early gastric cancer tissue. (C–D) IHC of ADARB1 in advanced gastric cancer tissue. (E–F) IHC of RBM15B in early gastric cancer tissue. (G–H) IHC of RBM15B in advanced gastric cancer tissue. (I–J) Sections were semi-quantitatively scored for ADARB1 or RBM15B staining patterns as follows: the staining extent in each core was scored as 1+ ( 75% staining of tumor cells). Additionally, the staining intensity was quantified as 0 (negative), 1+ (weak), 2+ (intermediate), or 3+ (strong). The final immunoreaction score was obtained by multiplying the intensity and extension values (range 0–12) and the samples were grouped as 1+ (score 0–2), 2+ (score 3–4), 3+ (score 6–8) and 4+ (score 9–12) staining. Meanwhile, for statistical purposes, scores of 3+ and 4+ was defined as high expression and the other final scores were considered as low expression, and then chi-squared test was used to compare the differences between high-expression and low-expression groups

Additional file 6 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 6: Fig. S6. Functional analysis of 194 RNA modification related genes and survival analysis of 300 samples in GSE62254 dataset. (A-C) GO analysis of 194 genes on molecular function (A), cellular component (B) and biological process (C). (D) Kaplan-Meier survival curve of cluster A and cluster B in GSE62254 dataset. Log-rank test was performed

Additional file 5 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 5: Fig. S5. Evaluation of immune infiltration of two clusters by Timer. ***p < 0.001

Additional file 4 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 4: Fig. S4. The correlation between 26 RNA modification writers and immune infiltration. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Additional file 10 of Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer

Additional file 10: Table S2. Clinical information of GSE62254 dataset

Roll-to-Roll Scalable Production of Ordered Microdomains through Nonvolatile Additive Solvent Annealing of Block Copolymers

A new method, “nonvolatile solvent vapor annealing” (NVASA), has been developed to anneal block copolymers during film deposition by controlling the solvent drying process. Precise amounts of high boiling point additive added to the polymer solution briefly remain in the polymer film after casting, leaving the film in a swollen state, increasing its chain mobility, and ultimately improving domain order. We demonstrated the effectiveness of NVASA on several block copolymer systems and used in situ grazing incidence small-angle X-ray scattering (GISAXS) to validate the ordering process during the self-assembly. The simplicity and reproducibility of the method is attractive for implementation in large-scale manufacturing processes such as roll-to-roll printing as swell ratio is easily controlled by the amount of additive used and separate annealing steps are not needed. This work potentially introduces a new way to quickly and cost effectively anneal block copolymers