Cell immunofluorescence for the observation of surface protein expression.

<p>Both vocal fold fibroblasts (VFFs) (A) and differentiated adipose-derived mesenchymal stem cells (dADSCs) (B) can express vimentin and fibronectin. Scale bar  =  50μm.</p

The cell morphology of vocal fold fibroblasts (VFFs).

<p>(A) and differentiated adipose-derived mesenchymal stem cells (dADSCs) (B). Both showed a spindle shape. Scale bar  =  100μm.</p

Diagram of the layered structure of the vocal fold (Hoechst staining).

<p>The canine vocal fold includes the epithelium (E), lamina propria (LP) and muscle (M). Scale bar  =  100μm.</p

DataSheet_1_Pomelo seed oil: Natural insecticide against cowpea aphid.docx

Cowpea aphid (Aphis craccivora Koch) is a plant pest that causes serious damage to vegetable crops. Extensive use of synthetic chemical pesticides causes deleterious effects on consumers as well as the environment. Hence, the search for environmentally friendly insecticides in the management of cowpea aphids is required. The present work aims to investigate the aphicidal activity of pomelo seed oil (PSO) on cowpea aphids, the possible insecticidal mechanisms, its chemical constituent profile, as well as the toxicity of its primary compounds. The results of the toxicity assay showed that PSO had significant insecticidal activity against aphids with a 72-hour LC50 value of 0.09 μg/aphid and 3.96 mg/mL in the contact and residual toxicity assay, respectively. The enzymatic activity of both glutathione S-transferase (GST) and acetyl cholinesterase (AChE) significantly decreased, as well as the total protein content, after PSO treatment, which suggested that the reduction of AChE, GST, and the total protein content in aphids treated with PSO might be responsible for the mortality of A. craccivora. The GC-MS analysis revealed that PSO contained limonene (22.86%), (9Z,12Z)-9,12-octadecadienoic acid (20.21%), n-hexadecanoic acid (15.79%), (2E,4E)-2,4-decadienal (12.40%), and (2E,4Z)-2,4-decadienal (7.77%) as its five major compounds. Furthermore, (9Z,12Z)-9,12-octadecadienoic acid showed higher toxicity to aphids than both PSO and thiamethoxam (positive control). This study emphasized the potential of PSO as a natural plant-derived insecticide in controlling cowpea aphids and also provided a novel approach for the value-added utilization of pomelo seed.</p

Co Nanoparticles Embedded in N‑Doped Carbon Nanotubes for Broadband Microwave Absorption

Herein, an efficient one-dimensional (1D) N-doped Co/C nanotube absorber was designed by a cobalt source and solvent ratio comodulation strategy. First, we explored the influences of different cobalt sources (CoCl2, Co(OAc)2, Co(NO3)2, and CoSO4) on the morphology and microwave absorption (MA) property, which showed that CoCl2 facilitated the formation of nanotubes with a relatively uniform diameter and length. Notably, the Co/C nanotubes displayed an ultrawide effective absorption bandwidth (EAB) of 6.885 GHz at 2.2 mm when the filling ratio was only 8 wt %. Then, by adjusting the solvent ratio of isopropanol (IPA) and water, Co/C showed a strong absorption of −44.51 dB with an EAB of 5.44 GHz at 2.35 mm. The outstanding MA performance is ascribed to the synergistic effect of dielectric and magnetic components, which is conducive to optimizing the impedance matching, thus broadening the EAB. In addition, the 1D nanostructure is good for constructing three-dimensional (3D) conductive networks and enhancing conductive loss. Since Co nanoparticles are wrapped in carbon nanotubes (CNTs), the confinement effect can effectively prevent the agglomeration of ferromagnetic nanoparticles, adjusting the MA performance. It is worth mentioning that the maximum Radar cross section (RCS) value of Co/C can be reduced by 28.8 dB m2. Furthermore, the RCS values are all lower than −10 dB m2 from −90 to 90° and the minimum RCS value can reach −46.8 dB m2. This work provides a strategy for designing lightweight MA absorbers with strong RL and ultrawide EAB

Stable Thermochromic Hydrogel for a Flexible and Wearable Zinc-Ion Yarn Battery with High-Temperature Warning Function

Hydrogel electrolytes are considered to be excellent substitutes for liquid electrolytes due to their safety and portability and are widely used in flexible and wearable electronics. In this work, we constructed hydrogels based on polyacrylamide (PAM), cotton cellulose nanofibers (CNFs), and carboxymethyl cellulose IV (CMC) with a semi-interpenetrating cross-linked network structure with excellent mechanical properties and high ionic conductivity for a thermochromic aqueous zinc-ion yarn battery, which exhibits excellent thermochromic cycling stability and outstanding electrochemical performance. It exhibits excellent thermochromic features during repeated cycling from 25 to 60 °C, delivers a high capacity of 383 mAh g–1 at 0.5 A g–1, and shows excellent cycle stability with 98% and 81% of the initial capacity retained after 1000 and 3500 cycles, respectively. More importantly, it has a very fast response speed to high temperature, which can intuitively provide the wearer with a high-temperature warning and effectively avoid low temperature scalding while meeting the energy supply. It offers great potential for practical wearable applications in high-temperature warning. Therefore, this work provides an approach and application for developing reliable, durable, and wearable smart batteries

Discovery of KLS-13019, a Cannabidiol-Derived Neuroprotective Agent, with Improved Potency, Safety, and Permeability

Cannabidiol is the nonpsychoactive natural component of <i>C. sativa</i> that has been shown to be neuroprotective in multiple animal models. Our interest is to advance a therapeutic candidate for the orphan indication hepatic encephalopathy (HE). HE is a serious neurological disorder that occurs in patients with cirrhosis or liver failure. Although cannabidiol is effective in models of HE, it has limitations in terms of safety and oral bioavailability. Herein, we describe a series of side chain modified resorcinols that were designed for greater hydrophilicity and “drug likeness”, while varying hydrogen bond donors, acceptors, architecture, basicity, neutrality, acidity, and polar surface area within the pendent group. Our primary screen evaluated the ability of the test agents to prevent damage to hippocampal neurons induced by ammonium acetate and ethanol at clinically relevant concentrations. Notably, KLS-13019 was 50-fold more potent and >400-fold safer than cannabidiol and exhibited an in vitro profile consistent with improved oral bioavailability