170 research outputs found
Students from the Medieval Hungarian Kingdom at Italian Universities
Classification accuracy of features combination on different mRNASeq datasets. (PDF 61Ă‚Â kb
Control of cell cycle entry and exiting from the second mitotic wave in the developing eye-2
Utant clones (D-F). No Dap-HB reporter activity (red in G-I) was observed in mutant clones. Although increased Dap-HB reporter expression was detected in the single mutant clones (Fig. 2D-F), no increased reporter activity was observed in , double mutant clones (red in J-L). Mutant cells are marked by the absence of GFP.<p><b>Copyright information:</b></p><p>Taken from "Control of cell cycle entry and exiting from the second mitotic wave in the developing eye"</p><p>http://www.biomedcentral.com/1471-213X/8/7</p><p>BMC Developmental Biology 2008;8():7-7.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267170.</p><p></p
Control of cell cycle entry and exiting from the second mitotic wave in the developing eye-3
) Quantification of BrdU positive cells in and mutant eye discs. BrdU positive cells within an area of 150 ÎĽm Ă— 50 ÎĽm along the SMW from 5 eye discs were counted. (G) Quantification of BrdU positive cells within a 30 ÎĽm Ă— 30 ÎĽm area in mutant clones or adjacent WT tissues. 10 independent mutant clones and adjacent WT tissues were analyzed.<p><b>Copyright information:</b></p><p>Taken from "Control of cell cycle entry and exiting from the second mitotic wave in the developing eye"</p><p>http://www.biomedcentral.com/1471-213X/8/7</p><p>BMC Developmental Biology 2008;8():7-7.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267170.</p><p></p
Control of cell cycle entry and exiting from the second mitotic wave in the developing eye-1
Ncer activity (red) is also upregulated in mutant clones spanning SMW. (G-I) In mutant background there is a significant number of mutant cells incorporating BrdU near SMW (red). Mutant clones are mark by absence of GFP and the arrows mark some BrdU cells in , double mutant clone. (J) Quantification of the number of mutant cells incorporating BrdU in the WT () or mutant background (, ). The average number of BrdU incorporating cells is normalized to the unit area (10pixels) and Con 1 and Con 2 indicate the average number of BrdU incorporating cells in the SMW of WT or eye discs adjacent to the mutant clones.<p><b>Copyright information:</b></p><p>Taken from "Control of cell cycle entry and exiting from the second mitotic wave in the developing eye"</p><p>http://www.biomedcentral.com/1471-213X/8/7</p><p>BMC Developmental Biology 2008;8():7-7.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267170.</p><p></p
Control of cell cycle entry and exiting from the second mitotic wave in the developing eye-5
Cyclin E level (red) is up-regulated. (G-I) In mutant cells, BrdU (red) is not incorporated in the SMW. (J-L) mutant cells have high level of Cyclin E protein (red). Mutant clones were marked by the absence of GFP (A-L). In all discs, anterior is to the left. White arrowheads in these and subsequent figures indicate the position of the SMW. The over-expression of Cyclin E (M) or Cdk2 (N) alone is not able to induce ectopic BrdU incorporation (red) in mutant clones. (O) Simultaneous over-expression of Cyclin E and Cdk2 induces large number of BrdU incorporation in mutant cells. MARCM clones were marked by the GFP (M-O).<p><b>Copyright information:</b></p><p>Taken from "Control of cell cycle entry and exiting from the second mitotic wave in the developing eye"</p><p>http://www.biomedcentral.com/1471-213X/8/7</p><p>BMC Developmental Biology 2008;8():7-7.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267170.</p><p></p
Control of cell cycle entry and exiting from the second mitotic wave in the developing eye-0
Cyclin E level (red) is up-regulated. (G-I) In mutant cells, BrdU (red) is not incorporated in the SMW. (J-L) mutant cells have high level of Cyclin E protein (red). Mutant clones were marked by the absence of GFP (A-L). In all discs, anterior is to the left. White arrowheads in these and subsequent figures indicate the position of the SMW. The over-expression of Cyclin E (M) or Cdk2 (N) alone is not able to induce ectopic BrdU incorporation (red) in mutant clones. (O) Simultaneous over-expression of Cyclin E and Cdk2 induces large number of BrdU incorporation in mutant cells. MARCM clones were marked by the GFP (M-O).<p><b>Copyright information:</b></p><p>Taken from "Control of cell cycle entry and exiting from the second mitotic wave in the developing eye"</p><p>http://www.biomedcentral.com/1471-213X/8/7</p><p>BMC Developmental Biology 2008;8():7-7.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267170.</p><p></p
DataSheet_1_The construction of a hypoxia-based signature identified CA12 as a risk gene affecting uveal melanoma cell malignant phenotypes and immune checkpoint expression.zip
Uveal melanoma (UM) is a deadly intraocular neoplasm in the adult population and harbors limited therapeutic effects from the current treatment. Here, we aimed to investigate the role of hypoxia in UM progress. We adopted the Cancer Genome Atlas data set as a training cohort and Gene Expression Omnibus data sets as validating cohorts. We first used consensus clustering to identify hypoxia-related subtypes, and the C1 subtype predicted an unfavorable prognosis and exhibited high infiltration of immunocytes and globally elevated immune checkpoint expression. Besides this, the patients with the C1 subtype were predicted to respond to the PD-1 treatment. By the least absolute shrinkage and selection operator algorithm, we constructed a hypoxia risk score based on the hypoxia genes and identified 10 genes. The risk score predicted patient survival with high performance, and the high-risk group also harbored high immunocyte infiltration and immune checkpoint expression. Furthermore, we confirmed that the risk genes were upregulated under hypoxia, and knockdown of CA12 inhibited the epithelial–mesenchymal transition process, clone formation ability, and G1/S phase transformation of the UM cells. The CD276 was also downregulated when CA12 knockdown was performed. These results validate the prognostic role of the hypoxia signature in UM and demonstrate that CA12 is a critical factor for UM cell progression as well as a target to improve immunotherapeutic effects. We believe our study contributes to the understanding of hypoxia’s roles in UM and provides a novel target that will benefit future therapeutic strategy development.</p
Enhanced Stability and Performance of Immobilized Lipase Using Hydrophobically Modified Single-Crystalline Ordered Macro–Microporous CuBTC as a Carrier Material
Single-crystalline
ordered macro–microporous CuBTC
(SOM–CuBTC)
is a promising carrier for lipase immobilization due to enhanced mass
transfer and stability toward fatty acids. However, the low yield
per mass of the template during the preparation process and the water
instability of the SOM–CuBTC carrier have posed significant
limitations on its practical applications. In this study, we addressed
these challenges by introducing a novel dual-solvent system consisting
of dimethyl sulfoxide (DMSO) and ethanol to obtain a stable precursor
solution with a concentration approximately 10 times higher than that
in previous literature, yielding 21.4 mg of SOM–CuBTC per gram
of the polystyrene template. However, the decomposition of SOM–CuBTC
in an aqueous system of lipase immobilization was observed. We explored
chemical vapor deposition and sol–gel methods for hydrophobic
modification on SOM–CuBTC. SOM–CuBTC coated by hydrophobic
polydimethylsiloxane (PDMS) via the sol–gel method possessed
excellent chemical stability and exhibited great potential for lipase
immobilization with a significant increase by 98.7% in the specific
activity. The obtained immobilized lipase not only showed improved
thermal stability and pH tolerance but also displayed excellent catalytic
performance in the synthesis process of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol
(OPL) by acidolysis. This work reveals the great potential of SOM–CuBTC
and provides new insights into the rational design of metal–organic
frameworks for enzyme immobilization in extensive applications
Construction of Furan Derivatives with a Trifluoromethyl Stereogenic Center: Enantioselective Friedel–Crafts Alkylations via Formal Trienamine Catalysis
An asymmetric Friedel–Crafts
alkylation reaction of 2-furfuryl
ketones with β-trifluoromethyl enones has been developed via
formal trienamine catalysis of a bifunctional primary amine-thiourea
substance derived from <i>L</i>-<i>tert</i>-leucine,
delivering the furan derivatives incorporating a stereogenic trifluoromethyl
(CF<sub>3</sub>) group in good to high yields with excellent enantioselectivity
Construction of Furan Derivatives with a Trifluoromethyl Stereogenic Center: Enantioselective Friedel–Crafts Alkylations via Formal Trienamine Catalysis
An asymmetric Friedel–Crafts
alkylation reaction of 2-furfuryl
ketones with β-trifluoromethyl enones has been developed via
formal trienamine catalysis of a bifunctional primary amine-thiourea
substance derived from <i>L</i>-<i>tert</i>-leucine,
delivering the furan derivatives incorporating a stereogenic trifluoromethyl
(CF<sub>3</sub>) group in good to high yields with excellent enantioselectivity
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