Paths to Positivity: Relational Trajectories and Interaction in Positive Stepparent-Stepchild Dyads

Stepfamilies are inherently complex family systems, marked by change, flexible boundaries, and early conflict. But the developmental pathways by which long-term stepparent relationships become positive require more study. We interviewed 38 stepchildren who had reached adulthood, to understand how their relationships with a stepparent became positive. Four relational trajectories defined these positive relationships: punctuated, consistent positive, progressive incline, and modulated turbulent. Distinctive communicative practices were associated with each trajectory, such as communicating assurances, “siding,” or revelations of character. In addition, the trajectories shared three common processes: responsiveness to stepchild vulnerability, stepparent “adding value” to the family, and maturation/ reframing of the past. Findings support the existence of multiple pathways to positivity and suggest that major fluctuations are experienced along the way. Findings are interpreted in light of existing research on stepfamily development and Afifi’s theory of resilience and relational load. Recommendations are offered for stepfamilies and professionals who serve them

Discourses of forgiveness and resilience in stepchild–stepparent relationships

Challenges and conflicts experienced by stepfamilies are well documented, but researchers are increasingly focused on communication processes that facilitate resilience in these relationships. In other contexts, communicating forgiveness has been linked to relational healing after transgressions or adversity. In the current study, the researchers sought to understand how stepchildren talk about the role of forgiveness in the development of positive adult stepchild–stepparent relationships. Data were drawn from interviews with adult stepchildren who have a positive relationship with a stepparent. Following an interpretive analysis, the researchers identified five themes representing the ways forgiveness was conceptualized and enacted in these positive stepchild–stepparent relationships: forgiveness as (a) healing family connections, (b) explicit negotiation, (c) maturation and acceptance, (d) a response to vulnerability and compassion, and (e) evidence of relational growth. Theoretical and practical applications for understanding and fostering resilient stepfamilies and the role of forgiveness are discussed

Therapeutic Targeting of Proteostasis in Amyotrophic Lateral Sclerosis—a Systematic Review and Meta-Analysis of Preclinical Research

Funding This work was supported by AMS: 210JMG 3102 R45620 and CSO and MNDS: 217ARF R45951. Medical Research Council (MRC UK; MR/L016400/1).Peer reviewedPublisher PD

Gravity, Two Times, Tractors, Weyl Invariance and Six Dimensional Quantum Mechanics

Fefferman and Graham showed some time ago that four dimensional conformal geometries could be analyzed in terms of six dimensional, ambient, Riemannian geometries admitting a closed homothety. Recently it was shown how conformal geometry provides a description of physics manifestly invariant under local choices of unit systems. Strikingly, Einstein's equations are then equivalent to the existence of a parallel scale tractor (a six component vector subject to a certain first order covariant constancy condition at every point in four dimensional spacetime). These results suggest a six dimensional description of four dimensional physics, a viewpoint promulgated by the two times physics program of Bars. The Fefferman--Graham construction relies on a triplet of operators corresponding, respectively to a curved six dimensional light cone, the dilation generator and the Laplacian. These form an sp(2) algebra which Bars employs as a first class algebra of constraints in a six-dimensional gauge theory. In this article four dimensional gravity is recast in terms of six dimensional quantum mechanics by melding the two times and tractor approaches. This "parent" formulation of gravity is built from an infinite set of six dimensional fields. Successively integrating out these fields yields various novel descriptions of gravity including a new four dimensional one built from a scalar doublet, a tractor vector multiplet and a conformal class of metrics.Comment: 27 pages, LaTe

Protector and friend: Turning points and discursive constructions of the stepparent role

Objective: To understand turning points (TPs) in the development of positive stepparent–stepchild communication and relationships. Background: Scholars stress the importance of communication in co-constructing healthy stepparent–stepchild relationships. The researchers focused on positive stepparenting via understanding transformational turning point (TP) events across time. Research questions explored how stepparents with an overall positive relationship with a stepchild characterize TPs and the discursive constructions of the stepparent role. Method: The team analyzed 877 pages of data from 37 in-depth interviews with stepparents who described self-identified TP events, reflected in visual graphs of 279 TPs. Results: Data were coded into 11 TP types, focused on structural and role changes for stepparents, co-constructed over time. The top three TP types were changes in household composition, communicating support through offering protection and being present/available, and role change, most frequently by functioning as a parent versus friend. All the TPs highlight discursive work to forge positive stepparenting roles. Conclusions: The findings extend earlier studies of stepchildren’s experiences and communication practices that ground resilience to manage relational resources through investments of quality time and enactment of social support. Implications: Applications suggest support for stepparents to have quality interactions with stepchildren and training to develop healthy communication practices and facilitate resilience

“Feeling Warmth and Close to Her”: Communication and Resilience Reflected in Turning Points in Positive Adult Stepchild–Stepparent Relationships

With the goal of understanding the development of positive stepchild–stepparent relationships, the researchers focused on turning points characterizing the interaction of adult stepchildren who have a positive bond with a stepparent. Engaging a relational turning points perspective, 38 stepchildren (males and females, ages 25 to 52 years old) who reported a positive stepparent relationship were interviewed, generating 269 turning points which were categorized into 15 turning point types and coded by valence. Turning points occurring most frequently were: prosocial actions, quality time, conflict/ disagreement, changes in household/family composition, and rituals. Findings are discussed, including implications for developing and enacting resilient and positive stepchild–stepparent relationships and future directions for researchers wanting to focus on positive family interaction

“Feeling Warmth and Close to Her”: Communication and Resilience Reflected in Turning Points in Positive Adult Stepchild–Stepparent Relationships

With the goal of understanding the development of positive stepchild–stepparent relationships, the researchers focused on turning points characterizing the interaction of adult stepchildren who have a positive bond with a stepparent. Engaging a relational turning points perspective, 38 stepchildren (males and females, ages 25 to 52 years old) who reported a positive stepparent relationship were interviewed, generating 269 turning points which were categorized into 15 turning point types and coded by valence. Turning points occurring most frequently were: prosocial actions, quality time, conflict/ disagreement, changes in household/family composition, and rituals. Findings are discussed, including implications for developing and enacting resilient and positive stepchild–stepparent relationships and future directions for researchers wanting to focus on positive family interaction

Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

<p>Abstract</p> <p>Background</p> <p>MicroRNAs (miRs) are non-coding RNA molecules involved in post-transcriptional regulation, with diverse functions in tissue development, differentiation, cell proliferation and apoptosis. miRs may be less prone to degradation during formalin fixation, facilitating miR expression studies in formalin-fixed paraffin-embedded (FFPE) tissue.</p> <p>Results</p> <p>Our study demonstrates that the TaqMan Human MicroRNA Array v1.0 (Early Access) platform is suitable for miR expression analysis in FFPE tissue with a high reproducibility (correlation coefficients of 0.95 between duplicates, p < 0.00001) and outlines the optimal performance conditions of this platform using clinical FFPE samples. We also outline a method of data analysis looking at differences in miR abundance between FFPE and fresh-frozen samples. By dividing the profiled miR into abundance strata of high (Ct<30), medium (30≤Ct≤35), and low (Ct>35), we show that reproducibility between technical replicates, equivalent dilutions, and FFPE <it>vs</it>. frozen samples is best in the high abundance stratum. We also demonstrate that the miR expression profiles of FFPE samples are comparable to those of fresh-frozen samples, with a correlation of up to 0.87 (p < 0.001), when examining all miRs, regardless of RNA extraction method used. Examining correlation coefficients between FFPE and fresh-frozen samples in terms of miR abundance reveals correlation coefficients of up to 0.32 (low abundance), 0.70 (medium abundance) and up to 0.97 (high abundance).</p> <p>Conclusion</p> <p>Our study thus demonstrates the utility, reproducibility, and optimization steps needed in miR expression studies using FFPE samples on a high-throughput quantitative PCR-based miR platform, opening up a realm of research possibilities for retrospective studies.</p

Weyl's Gauge Invariance: Conformal Geometry, Spinors, Supersymmetry, and Interactions

We extend our program, of coupling theories to scale in order to make their Weyl invariance manifest, to include interacting theories, fermions and supersymmetric theories. The results produce mass terms coinciding with the standard ones for universes that are Einstein, but are novel in general backgrounds. They are generalizations of the gravitational couplings of a conformally improved scalar to fields with general scaling and tensor properties. The couplings we find are more general than just trivial ones following from the conformal compensating mechanisms. In particular, in the setting where a scale gauge field (or dilaton) is included, masses correspond to Weyl weights of fields organized in ``tractor'' multiplets. Breitenlohner--Freedman bounds follow directly from reality of these weights. Moreover, massive, massless and partially massless theories are handled in a uniform framework. Also, bona fide Weyl invariant theories (invariant without coupling to scale) can be directly derived in this approach. The results are based on the tractor calculus approach to conformal geometry, in particular we show how to handle fermi fields, supersymmetry and Killing spinors using tractor techniques. Another useful consequence of the construction is that it automatically produces the (anti) de Sitter theories obtained by log-radial reduction of Minkowski theories in one higher dimension. Theories presented in detail include interacting scalars, spinors, Rarita--Schwinger fields, and the interacting Wess--Zumino model.Comment: 31 pages, LaTe

Local Unit Invariance, Back-Reacting Tractors and the Cosmological Constant Problem

When physics is expressed in a way that is independent of local choices of unit systems, Riemannian geometry is replaced by conformal geometry. Moreover masses become geometric, appearing as Weyl weights of tractors (conformal multiplets of fields necessary to keep local unit invariance manifest). The relationship between these weights and masses is through the scalar curvature. As a consequence mass terms are spacetime dependent for off-shell gravitational backgrounds, but happily constant for physical, Einstein manifolds. Unfortunately this introduces a naturalness problem because the scalar curvature is proportional to the cosmological constant. By writing down tractor stress tensors (multiplets built from the standard stress tensor and its first and second derivatives), we show how back-reaction solves this naturalness problem. We also show that classical back-reaction generates an interesting potential for scalar fields. We speculate that a proper description of how physical systems couple to scale, could improve our understanding of naturalness problems caused by the disparity between the particle physics and observed, cosmological constants. We further give some ideas how an ambient description of tractor calculus could lead to a Ricci-flat/CFT correspondence which generalizes the AdS side of Maldacena's duality to a Ricci-flat space of one higher dimension.Comment: 20 pages, 2 figure