Additional file 4: Figure S1. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

miR-326 and ARRB1 share a common promoter upstream to ARRB1. Figure S2. Flowchart for the shortlisting of transcription factors regulated by the PI3 kinase pathway. (PPTX 303 kb

Additional file 7: Table S6. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

Gene regulated by miR-326 overexpression at 72 h interval. (XLSX 22 kb

Additional file 6: Table S5. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

Gene regulated by miR-326 overexpression at 48 h interval. (XLSX 44 kb

Additional file 5: Table S4. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

List of transcription factors regulated by PI3 kinase pathway that can potentially regulate ARRB1 locus. (XLSX 13 kb

Additional file 8: Table S7. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

List of genes downregulated upon miR-326 overexpression, upregulated in GBM and bearing binding sites for miR-326. (XLSX 12 kb

Inhibition of MAPK/PI3K pathways and glioma cell migration by miR-219-5p are mediated by EGFR.

<p>U87 parental (par) (<b>A</b>), U87 (wt-EGFR) and U87 (ΔEGFR) (<b>B</b>) cells were transfected with pre-miR-219-5p (P-219) or negative control pre-miRs (P-neg). After 72 hrs of pre-miR transfection, cell lysate was prepared and subjected to the western blotting for EGFR, phosphor-ERK1/2 (Tyr202/Tyr204) and total ERK1/2; phosphor-Akt (Ser473), total Akt. <b>C.</b> U87 parental (par), U87 wt-EGFR cells and U87 (ΔEGFR) were transfected with negative control pre-miRs (P-neg) or pre-miRs for miR-219-5p (P-219). After 72 hrs of transfection, cells were counted and plated for matrigel migration assay and after 18 hrs, cells were fixed, stained with crystal violet, photographed. <b>D.</b> Quantification of <b>C.</b> Average cell number ± SD was plotted. p value was calculated by Students' T test in MS excel. NS represents Non-Significant p value.</p

Additional file 2: Table S2. of PI3 kinase pathway regulated miRNome in glioblastoma: identification of miR-326 as a tumour suppressor miRNA

List of miRNAs regulated upon PI3 kinase pathway inhibition and their regulation in GBM. (XLSX 12 kb

SUPPLEMENT_CLINICAL – Supplemental material for L1CAM Immunopositivity in Anaplastic Supratentorial Ependymomas: Correlation With Clinical and Histological Parameters

Supplemental material, SUPPLEMENT_CLINICAL for L1CAM Immunopositivity in Anaplastic Supratentorial Ependymomas: Correlation With Clinical and Histological Parameters by Pooja Chavali, Shilpa Rao, Sravya Palavalasa, Nandeesh Bevinahalli, Yasha T. C. Muthane, Nishanth Sadashiva and Vani Santosh in International Journal of Surgical Pathology</p

Levels of 18 discriminatory cytokines in normal and GBM sera of the training set.

<p>^<b>a</b>Cytokine profiling (n = 48) was carried out using bead array method. The levels of 18 discriminatory cytokines of the training set was converted to differential log 2 ratio by dividing the individual sample value with mean of all normal samples for a given cytokine. In this table, median of differential log 2 ratio for a given cytokine and standard error are shown.</p><p>^#The abundance of cytokines in GBM sera when compared to normal sera. “High” refers to cytokine present in elevated levels and “Low” refers to cytokine present in lower levels in GBM sera when compared to normal sera.</p><p>^<b>b</b>The TCGA microarray data which is publically available was used to check the transcript levels of 18 differentially abundant cytokines. Non-parametric t-test was conducted with FDR correction using log 2 ratio of normal brain tissue and GBM tumor tissue to identify significant differentially regulated cytokines at transcript level. The regulation, p value and log 2 fold change are provided in the table. “Up” refers to up-regulated and “Down” refers to down-regulated in GBM when compared to normal brain.</p><p>NS refers to non-significant.</p><p>Levels of 18 discriminatory cytokines in normal and GBM sera of the training set.</p

The diagnostic accuracy, sensitivity and specificity of 18-cytokine signature in different sample sets.

<p>^<b>a</b>Sensitivity = (the number of positive samples predicted)/(the number of true positives)</p><p>^<b>b</b>Specificity = (the number of negative samples predicted)/(the number of true negatives)</p><p><b>Note:</b> Values within the parentheses represents (number of samples predicted correctly)/(total number of samples)</p><p>The diagnostic accuracy, sensitivity and specificity of 18-cytokine signature in different sample sets.</p