Pierre Loti'nin yüzüncü doğum yıldönümü

Taha Toros Arşivi, Dosya Adı: Pierre Lotiİstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033

Substrate variety and host preference of the epizoic limpet <i>Lottia tenuisculpta</i> (Patellogastropoda: Lottiidae)

Substrate variety and host preference of the epizoic limpet Lottia tenuisculpta were investigated based on specimen and field observations. Specimen observation revealed the presence of the limpet on 33 species of Mollusca and two species of Crustacea, and it predominantly used Tegulidae and Muricidae throughout its geographic range. Some of the substrate molluscs had radula traces and home scars probably engraved by L. tenuisculpta. Field observations conducted on the rocky shore of Shirahama, Wakayama, Japan, revealed the presence of L. tenuisculpta on 12 species of molluscs. The tegulid Omphalius nigerrimus and the muricid Reishia clavigera were its major substrates in the locality. The results of both sets of observations suggest that L. tenuisculpta is a host-general facultative epizoite and its host preference is family-specific. The choice of host species by L. tenuisculpta might be determined by the abundance and morphological and biological restrictions of the preferred substrates in each locality. The host-general and facultative nature of the limpet allows it to show geographical host conversion and contribute to favouring epizoic behaviour. Home scars of L. tenuisculpta on the hosts suggest that the limpet inhabits the same individual of the substrate species for an extended period.</p

Bayesian maximum credibility tree (100 x 10⁶ generations trees sampled every 1000 generations) in the evolution of the Patellogastropoda.

<p>Support values and posterior probabilities are marked in each node MP/ML/BI. Scale at the x-axis represents estimated age in million years and grey bars represent 95% highest posterior density intervals. * indicates the separation of the major clades (A, B, and C) within <i>Cellana</i> and ** indicates the separation between clades A and B.</p

Long-distance dispersal events in the biogeography of <i>Cellana</i>.

<p>A) red line, colonization of Hawaii (Haw) during the Miocene from a North-Western Pacific source (N-WP1); B) blue line, colonization of Juan Fernández Archipelago (JFI) during the Mio-Pliocene from a New Zealand source (NZ), yellow arrow head, colonization of New Zealand sub-Antarctic Islands (SAI) during the Plio-Pleistocene from a New Zealand South Island source (NZSI). Whether the colonization of French Polynesia represents a long-distance dispersal event or a stepping-stone mediated process is still uncertain (?).</p

Current distribution of <i>Cellana</i> along the Indo-Pacific showing sampling localities of <i>Cellana</i>, <i>Nacella</i> and Outgroups.

<p>Current distribution of <i>Cellana</i> along the Indo-Pacific showing sampling localities of <i>Cellana</i>, <i>Nacella</i> and Outgroups.</p

Bayesian maximum credibility tree (100 x 10⁶ generations trees sampled every 1000 generations) showing the recognized clades in the evolution of <i>Cellana</i>.

<p>Each clade was named according to the current distribution of their representatives.</p

Patellogastropod species, specimens, accession numbers, and sampling localities included in the study.

<p>Specimen identifications were based on Powell [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170103#pone.0170103.ref014" target="_blank">14</a>], Valdovinos and Rüth [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170103#pone.0170103.ref070" target="_blank">70</a>], Nakano and Ozawa [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170103#pone.0170103.ref017" target="_blank">17</a>], and González-Wevar et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170103#pone.0170103.ref018" target="_blank">18</a>]. New COI sequences obtained in this study are marked in bold. Other Accession Numbers and their respective sources are indicated.</p

DataSheet_1_Complete mitochondrial genomes of the “Acmaeidae” limpets provide new insights into the internal phylogeny of the Patellogastropoda (Mollusca: Gastropoda).docx

The subclass Patellogastropoda (called “true limpets”) is one of the most primitive groups of the Gastropoda and contains approximately 350 species worldwide. Within this subclass, internal phylogeny among family members, including relationships of the “Acmaeidae” with other patellogastropod families, remains incompletely clarified. Here, we newly determined two complete mitochondrial genome sequences of “Acmaeidae” (Acmaea mitra and Niveotectura pallida) and one sequence from Lottiidae species (Discurria insessa) and combined them with mitochondrial genome sequences of 20 other published limpet species for phylogenetic analysis of the sequence dataset (nucleotides and amino acids) of 13 protein-coding genes using maximum likelihood and Bayesian inference methods. The resulting phylogenetic trees showed monophyly of Patellogastropoda species that were subsequently subdivided into two clades [clade I (Nacellidae, Pectinodontidae, Acmaeidae, and Patellidae) and clade II (Eoacmaeidae and Lottiidae)]. The sister relationship between the Acmaeidae and Pectinodontidae species revealed by phylogenetic analysis was also supported by sharing their similar gene arrangement patterns, which differ substantially from those of clade II members including the Lottiidae species. The polyphyletic relationship between Acmaeidae (grouped with Pectinodontidae as a sister taxon in clade I) and Lottiidae species (grouped with Eoacmaeidae in clade II) corroborates that they are phylogenetically distinct from each other. This mitochondrial genome phylogeny contradicts previous morphology-based hypotheses, yet highlights that Acmaeidae and Pectinodontidae are the most closely related. Further in-depth analysis of the complete mitochondrial genome sequences based on a broad range of samples including those from relatively unstudied and/or underrepresented taxa is required to fully understand the mitochondrial genome evolution and a more comprehensive phylogeny among the major groups of the Patellogastropoda.</p

Raw data of eDNA metabarcoding of environmental waters from Misaki, Japan, and custom reference fasta files of 16S rRNA of metazoans and ophiuroids

A fasta files of  8,975,113 sequences (Supplementary material 1) of mitochondrial 16S rRNA gene for metazoans which was downloaded from INSD as of 17 December 2021 and was alligned by using MEGA7. </p

Raw data of eDNA metabarcoding of environmental waters from Misaki, Japan, and custom reference fasta files of 16S rRNA of metazoans and ophiuroids

The raw sequence data of eDNA metabacording obtained from aquarium tank, pier, Moroiso (bottom and surface), 80 m and 250-270 m.  </p