Physiological and protein responses in leaves of <i>Nitraria billardieri</i> seedlings to moderate salt stress

Salt stress is a major environmental factor affecting plant growth and geographical distribution. Halophytes are considered valuable resources for investigating plant tolerance mechanisms. The halophyte Nitraria billardieri is widely distributed in saline soil in Australia and China. To investigate salt stress-induced changes at the physiological and molecular levels in N. billardieri, three-month-old seedlings were subjected to salt stress treatments. The physiological and biochemical analyses showed that N. billardieri seedlings could adapt to and strongly tolerate salt stress by accumulating soluble sugars and proline as organic osmolytes and increasing the activities of antioxidative enzymes. Comparative proteomic and metabolic pathway analyses revealed 130 differentially expressed proteins, which displayed various response patterns under salt stress. A protein interaction analysis found that the interaction consisting of amino acid and carbohydrate metabolism coupled with redox homeostasis and protein synthesis may play an essential role in response to salt stress in N. billardieri seedlings. Overall, salt stress treatment disrupted a cascade of normal metabolic programing and subsequently caused oxidative and osmotic stress by altering protein fates, signal transduction and redox homeostasis.</p

Motif distributions of the angiosperm PIN1 sequences.

<p>A schematic representation of motifs obtained using MEME within the sequences is displayed. Different motifs are highlighted by different colored boxes, Details concerning individual motifs are given in Figure S3.</p

Additional file 2 of Integrated metabolome and transcriptome analysis unveils novel pathway involved in the fruit coloration of Nitraria tangutorum Bobr.

Additional file 2: Supplementary Table 1. The raw data for the detected metabolic species in our manuscript. Supplementary Table 2. Differential metabolic species among the JC and NT. Supplementary Table 3. Summary of transcriptome sequencing data transcriptome assembly. Supplementary Table 4. Details of all the gene annotation. Supplementary Table 5. KOG function annotation of all unigenes. Supplementary Table 6. KEGG pathway annotation of all unigenes. Supplementary Table 7. Details of all the different expression genes in JC and NT.  Supplementary Table 8. KEGG pathways in which differentially expressed genes are involved. Supplementary Table 9. All DEGs of anthocyanin biosynthesis pathway. The different expression coding genes of key enzyme anthocyanin biosynthesis pathway after WGCNA. Supplementary Table 10. Statistical table of TFs in JC and NT. Supplementary Table 11. Primers used in qRT-PCR in this study

Additional file 2 of Genome-wide identification of the NAC gene family and its functional analysis in Liriodendron

Additional file 2: Table S2. FPKM values of LcNACs under heat, drought and cold stress

Additional file 3 of Genome-wide identification of the NAC gene family and its functional analysis in Liriodendron

Additional file 3: Table S3. The specific primers used in the qRT-PCR analysis for the selected LcNACs

It's Christmas Carol (2006)

1. Poster, 2. Photo, 3. Photo, 4. Photo, 5. Photo, 6. Photo, 7. Photo, 8. Photo, 9. Photo, 10. Photo, 11. Photo, 12. Photo, 13. Photo, 14. Photo, 15. Program, 16. Press Release English, 17. Press Release FrenchArchival file for the Glendon College production of It's Christmas Carol, written and directed by Nicole Toogood. The play was performed November 30th - December 9th, 2006

Site model (M7 vs. M8) test for each family PIN1 genes.

<p>lnL: the log-likelihood difference between the two models; 2Δl: twice the log-likelihood difference between the two models.</p><p>*: In the analysis of Poaceae, the P-value is less than the significance level 0.05, indicating that the M8 model fitted the data better than M7 model. However, the estimate of ω in M8 was less than (99% sites) or equal to 1 (1% sites), indicating no positive selection.</p

Additional file 5 of Genome-wide identification of the NAC gene family and its functional analysis in Liriodendron

Additional file 5: Table S5. The conserved motifs of the genes in the LcNAC gene family are determined by MEME

Additional file 1 of Integrated metabolome and transcriptome analysis unveils novel pathway involved in the fruit coloration of Nitraria tangutorum Bobr.

Additional file 1: Figure S1. The two principal components of YT VS RT (A) and YM VS RM B. Figure S2. Total ion flow diagram of QC samples and the multi-peak graphs of MRM metabolites. A Total ion flow diagram of QC samples (Positive ions). B Total ion flow diagram of QC samples (Negative ions). C The multi-peak graphs of MRM metabolites (Positive ions). D The multi-peakgraphs of MRM metabolites (Negative ions). Figure S3. Annotate all of the assembled genes in accordance with the public databases GO, Swiss-Prot, NR, COG/KOG, Trembl and KEGG. Figure S4. Unigenes were classified in the KOG database. Figure S5. The statistics of the number of differentially expressed genes and cluster analysis of transcription factors. A Statistics of the number of upregulated and downregulated DEG in the four groups of samples. The Abscissa represents the comparison between samples (groups), and the ordinate indicates the significant differentially expressed genes detected. B-C The sunburst chart of transcription factors associated with anthocyanin biosynthesis. The quantitative distribution of differentially expressed TFs in YM VS RM (B) and YT VS RT C. Figure S6. Top 20 of differential genes for KEGG enrichment. The ordinate is Pathway. The horizontal axis is the enrichment factor (the number of differences in this Pathway divided by all Numbers). The size is the quantity, the redder the color, the smaller the P/Q value

Additional file 1 of Genome-wide identification of the NAC gene family and its functional analysis in Liriodendron

Additional file 1: Table S1. The Fragments Per Kilobase per million mapped fragments (FPKM) data of NAC genes at different stages of somatic embryos