Predicted posterior median CD4 counts trajectory by covariate strata for the slope of CD4 count model, with 95% prediction intervals.

A. baseline CD4 count (cells/μL), B. sex, C. baseline age (years), D. baseline log10 VL (copies/mL). Median predicted CD4 counts from model 1, is plotted. The red, black or blue dots represent predicted median CD4 count at different time points, and the bars are the whiskers.</p

The effect of changing from a Gaussian to a skew-normal on the estimated regression coefficients, with 95% credible intervals, in the slope model.

The effect of changing from a Gaussian to a skew-normal on the estimated regression coefficients, with 95% credible intervals, in the slope model.</p

Posterior mean odds ratios and 95% credible intervals of the regression coefficients for the binary longitudinal models with response: CD4 counts ≥500 cells/μL.

Posterior mean odds ratios and 95% credible intervals of the regression coefficients for the binary longitudinal models with response: CD4 counts ≥500 cells/μL.</p

Posterior means and 95% credible intervals of the regression coefficients for the slope of CD4 count models.

Posterior means and 95% credible intervals of the regression coefficients for the slope of CD4 count models.</p

Predicted posterior median probability of having ≥500 cells/μL CD4 counts by covariate strata, with 95% prediction intervals.

A) baseline CD4 count (cells/μL), B) sex, C) Baseline age (years), D) baseline log10 viral load (copies/mL). Median predicted probability of having CD4 count ≥500 cells/μL from model 6. The red, black or blue dots represent predicted probability of having CD4 count ≥500 cells/μL at different time points, and the bars are the whiskers.</p

Estimating informative priors used for slope of CD4 count model.

Estimating informative priors used for slope of CD4 count model.</p

The effect of changing from a normal to a skew-normal distribution on the random-effects on the regression coefficients, with 95% credible intervals, in the asymptote model.

The effect of changing from a normal to a skew-normal distribution on the random-effects on the regression coefficients, with 95% credible intervals, in the asymptote model.</p

DataSheet_1_Comparison of platelet-and endothelial-associated biomarkers of disease activity in people hospitalized with Covid-19 with and without HIV co-infection.docx

IntroductionSARS-CoV-2 elicits a hyper-inflammatory response that contributes to increased morbidity and mortality in patients with COVID-19. In the case of HIV infection, despite effective anti-retroviral therapy, people living with HIV (PLWH) experience chronic systemic immune activation, which renders them particularly vulnerable to the life-threatening pulmonary, cardiovascular and other complications of SARS-CoV-2 co-infection. The focus of the study was a comparison of the concentrations of systemic indicators o\f innate immune dysfunction in SARS-CoV-2-PCR-positive patients (n=174) admitted with COVID-19, 37 of whom were co-infected with HIV.MethodsParticipants were recruited from May 2020 to November 2021. Biomarkers included platelet-associated cytokines, chemokines, and growth factors (IL-1β, IL-6, IL-8, MIP-1α, RANTES, PDGF-BB, TGF-β1 and TNF-α) and endothelial associated markers (IL-1β, IL-1Ra, ICAM-1 and VEGF).ResultsPLWH were significantly younger (p=0.002) and more likely to be female (p=0.001); median CD4+ T-cell count was 256 (IQR 115 -388) cells/μL and the median HIV viral load (VL) was 20 (IQR 20 -12,980) copies/mL. Fractional inspired oxygen (FiO2) was high in both groups, but higher in patients without HIV infection (p=0.0165), reflecting a greater need for oxygen supplementation. With the exception of PDGF-BB, the levels of all the biomarkers of innate immune activation were increased in SARS-CoV-2/HIV-co-infected and SARS-CoV-2/HIV-uninfected sub-groups relative to those of a control group of healthy participants. The magnitudes of the increases in the levels of these biomarkers were comparable between the SARS-CoV-2 -infected sub-groups, the one exception being RANTES, which was significantly higher in the sub-group without HIV. After adjusting for age, sex, and diabetes in the multivariable model, only the association between HIV status and VEGF was statistically significant (p=0.034). VEGF was significantly higher in PLWH with a CD4+ T-cell count >200 cells/μL (p=0.040) and those with a suppressed VL (p=0.0077).DiscussionThese findings suggest that HIV co-infection is not associated with increased intensity of the systemic innate inflammatory response during SARS-CoV-2 co-infection, which may underpin the equivalent durations of hospital stay, outcome and mortality rates in the SARS-CoV-2/HIV-infected and -uninfected sub-groups investigated in the current study. The apparent association of increased levels of plasma VEGF with SARS-CoV-2/HIV co-infection does, however, merit further investigation.</p

DataSheet_1_Despite delayed kinetics, people living with HIV achieve equivalent antibody function after SARS-CoV-2 infection or vaccination.docx

The kinetics of Fc-mediated functions following SARS-CoV-2 infection or vaccination in people living with HIV (PLWH) are not known. We compared SARS-CoV-2 spike-specific Fc functions, binding, and neutralization in PLWH and people without HIV (PWOH) during acute infection (without prior vaccination) with either the D614G or Beta variants of SARS-CoV-2, or vaccination with ChAdOx1 nCoV-19. Antiretroviral treatment (ART)–naïve PLWH had significantly lower levels of IgG binding, neutralization, and antibody-dependent cellular phagocytosis (ADCP) compared with PLWH on ART. The magnitude of antibody-dependent cellular cytotoxicity (ADCC), complement deposition (ADCD), and cellular trogocytosis (ADCT) was differentially triggered by D614G and Beta. The kinetics of spike IgG-binding antibodies, ADCC, and ADCD were similar, irrespective of the infecting variant between PWOH and PLWH overall. However, compared with PWOH, PLWH infected with D614G had delayed neutralization and ADCP. Furthermore, Beta infection resulted in delayed ADCT, regardless of HIV status. Despite these delays, we observed improved coordination between binding and neutralizing responses and Fc functions in PLWH. In contrast to D614G infection, binding responses in PLWH following ChAdOx-1 nCoV-19 vaccination were delayed, while neutralization and ADCP had similar timing of onset, but lower magnitude, and ADCC was significantly higher than in PWOH. Overall, despite delayed and differential kinetics, PLWH on ART develop comparable responses to PWOH, supporting the prioritization of ART rollout and SARS-CoV-2 vaccination in PLWH.</p