26 research outputs found

    VP1-specific competition of polyomavirus seroresponses.

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    <p>Serial dilutions of four serum samples were preincubated with soluble recombinant fusion protein lysate containing GST.tag alone (black), GST-HPyV6 VP1.tag (orange), GST-HPyV7 VP1.tag (blue) or GST-TSPyV, GST-MCPyV, GST-BKPyV, GST-HPyV9 (grey). The HPyV6 (left part of the figure) and HPyV7 (right part of the figure) VP1 seroreactivity was measured by the Bio-Plex 100 analyzer. MFI stands for median fluorescent intensity.</p

    Age-specific seroreactivity against MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>The age-specific seroreactivity is measured amongst the respective seropositive individuals. The horizontal line in each boxplot represents the median MFI value. The box includes the 25%–75% MFI value range and the whiskers the 5%–95% MFI value range. Outliers are represented as a circle and extreme outliers as an asterisk.</p

    Seropositivity of MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>Seropositivity is measured for the total study population divided into 14 different age groups. Error bars indicate 95% confidence intervals.</p

    Seroresponses measured against VP1 of MCPyV, HPyV6, HPyV7, TSPyV, HPyV9 and BKPyV.

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    <p>Presented are seroresponses of 799 Australian immunocompetent individuals. Each circle corresponds to the mean fluorecence intensity (MFI) measured for an individual serum sample in the Bio-Plex 100 analyzer. The cut-off value calculated for each polyomavirus as explained in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081078#pone.0081078.s001" target="_blank">Figure S1</a></b> is depicted as a blue line.</p

    Toscana virus tree showing H.sapiens-wt/Italy/2014/Genotype-A_BB8 segments detected in a returned Australian traveller

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    Toscana virus (TOSV) is identified in sandflies, animals and humans around the Mediterranean Sea. TOSV has not been reported in Australia. During investigations of cerebrospinal fluid samples from patients with encephalitis, a TOSV was identified in a traveller returning to Australia from Europe. <br>TOSV should be considered, especially during May to October, in travellers to Australia who embarked in countries in and around the Mediterranean Sea and who subsequently present for medical care because of neurological symptoms.<br><div><p>LEGEND.</p><p>Phylogenetic representation of complete and near complete Toscana virus (TOSV) lineage L (1a), M (1b) and S (1c) gene nucleotide segments compared to a Sandfly fever Naples virus (SFNV). Lineage A and B strains are indicated by blue (solid outline) and green boxes (dashed outline), respectively. A circle indicates the TOSV strain H.sapiens-wt/Italy/2014/Genotype-A_BB8, described here. MEGA7 was used to construct Neighbor-Joining trees with distances calculated using the p-distance method (500 bootstraps; percentage replicate tree branching patterns are shown next to initial branches). </p></div

    HRV-QPM detections and association of co-morbidities and severity of respiratory illness upon discharge.

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    <p>HMPV-Human metapneumovirus, HCoV-229E or -NL63-Human coronaviruses, HBoV-Human bocavirus, WUPyV-WU polyomavirus. Labs-Myco/Pert- laboratory testing for <i>Mycoplasma spp.</i> or <i>Bordetella pertussis.</i><sup>1</sup>Steroids, <sup>2</sup>adrenalin or <sup>3</sup>bronchodilators administered, <sup>4</sup>Comparison of severity score using Wilcoxon rank-sum test for HRV-QPM cases with and without co-detection, p = 0.88. <sup>5</sup>Recorded as ‘zero’ due to the patient being admitted for cardiac surgery and all criteria were in relation to the surgery and not the ARTI. <sup>*</sup> Not admitted. CF-cystic fibrosis. NC-No chart could be obtained for review, CHD-congenital heart disease. NN-No notes were available from presentation during the preliminary study.</p

    Criteria used to determine the severity[60] of respiratory illness upon discharge, in patients presenting to Queensland hospitals who tested positive for HRV-QPM.

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    1<p>Respiratory support by oxygen therapy and/or mechanical ventilation, <sup>2</sup>Supplementary fluids delivered by either intravenous and/or nasogastric route, <sup>3</sup><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001847#pone.0001847-Wainwright1" target="_blank">[61]</a>.</p
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