13 research outputs found

    Myofibrillar adenosine triphosphatase (mATPase) staining

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with mATPase (pH10.7). Lightly and darkly stained muscle fibers were classified as type I and type II muscle fibers, respectively. Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Dihydropyridine receptor alpha 1 (DHPRα1) localization

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with anti-DHPRα1 antibody. Asterisks indicate normal immunoreactivity (IR) in the cytoplasm and subsarcolemma of DHPRα1 (a-d, i-p). Arrows indicate muscle fiber with ectopic DHPRα1 IR (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Sarcoplasmic reticulum calcium ion ATPase 2a (SERCA2a) localization

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with anti-SERCA2a antibody. Asterisks indicate normal immunoreactivity (IR) of SERCA2a in the cytoplasm (a-d, i-p). Arrows indicate muscle fiber with ectopic IR of SERCA2a (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Junctophilin 1 (JPH1) localization

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with anti-JPH1 antibody. Asterisks indicate normal immunoreactivity (IR) to JPH1 in the cytoplasm and subsarcolemma (a-d, i-p). Arrows indicate muscle fiber with ectopic JPH1 IR (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Hematoxylin and eosin (HE) staining

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with HE. Arrowheads indicate spikes and lakes in cytoplasm (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Sarcoplasmic reticulum calcium ion ATPase 1 (SERCA1) localization

    No full text
    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with anti-SERCA1 antibody. Asterisks indicate normal immunoreactivity (IR) of SERCA1 in the cytoplasm (a-d, i-p). Arrows indicate muscle fiber with ectopic IR of SERCA1 (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Ryanodine receptor 1 (RyR1) localization

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with anti-RyR1 antibody. Asterisks indicate normal immunoreactivity (IR) in the cytoplasm and subsarcolemma of RyR1 (a-d, i-p). Arrows indicate muscle fiber with ectopic RyR1 IR (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p

    Modified Gomori’s trichrome staining

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    Cross-sections of the gastrocnemius (a-h) and soleus (i-p) muscles were stained with modified Gomori’s trichrome. Arrowheads indicate red materials in cytoplasm (e-h). Scale bar = 50 μm. GAS, gastrocnemius muscle; SOL, soleus muscle; 3M, 3-month-old mice; 24M, 24-month-old mice.</p
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