45 research outputs found

    Relationship between left main and left anterior descending arteries bifurcation angle and coronary artery calcium score in chronic kidney disease: A 3-dimensional analysis of coronary computed tomography - Fig 1

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    <p><b>A.</b> Schematic representation of the LM-LAD arteries angle measurements. We identified the lines that delimited the LM-LAD angle, using the centre lines of the LM and LAD arteries on the volume rendering image. <b>B, C.</b> Representative example of mild coronary calcifications in a 69-year-old man. The LM–LAD arteries angle measured 23.9° and the CACS in the LM-LAD arteries was 14.7. <b>D, E.</b> Representative example of severe coronary calcifications in a 63-year-old man. The LM–LAD arteries angle measured 37.1° and the CACS in the LM-LAD arteries was 783. LM = left main artery; LAD = left anterior descending artery; LCX = left circumflex artery.</p

    Behavioral and Proteomic Analysis of Stress Response in Zebrafish (<i>Danio rerio</i>)

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    The purpose of this study is to determine the behavioral and proteomic consequences of shock-induced stress in zebrafish (<i>Danio rerio</i>) as a vertebrate model. Here we describe the behavioral effects of exposure to predictable and unpredictable electric shock, together with quantitative tandem mass tag isobaric labeling workflow to detect altered protein candidates in response to shock exposure. Behavioral results demonstrate a hyperactivity response to electric shock and a suppression of activity to a stimulus predicting shock. On the basis of the quantitative changes in protein abundance following shock exposure, eight proteins were significantly up-regulated (HADHB, hspa8, hspa5, actb1, mych4, atp2a1, zgc:86709, and zgc:86725). These proteins contribute crucially in catalytic activities, stress response, cation transport, and motor activities. This behavioral proteomic driven study clearly showed that besides the rapid induction of heat shock proteins, other catalytic enzymes and cation transporters were rapidly elevated as a mechanism to counteract oxidative stress conditions resulting from elevated fear/anxiety levels

    Relationship between left main and left anterior descending arteries bifurcation angle and coronary artery calcium score in chronic kidney disease: A 3-dimensional analysis of coronary computed tomography

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    <div><p>Background</p><p>A high coronary artery calcium score (CACS) predicts a poor prognosis in patients with coronary artery disease. We examined the relationship between the bifurcation angle and the CACS of the left main (LM) and left anterior descending (LAD) arteries in patients suffering from chronic kidney disease (CKD).</p><p>Methods</p><p>We analyzed the data of 121 patients who underwent coronary computed tomography between October 2014 and June 2015 and whose estimated glomerular filtration rate (eGFR) was <60 ml/min/1.73 m<sup>2</sup>. The LM-LAD bifurcation angle was measured by 3-dimensional coronary computed tomography. The CACS of the LM-LAD arteries was also calculated. We excluded stent recipients and patient who had undergone coronary artery bypass graft surgery.</p><p>Results</p><p>In the overall sample, the mean ± standard deviation (range) LM-LAD bifurcation angle was 35.9 ± 11.4° (6.8–79.4°) and mean CACS was 227 ± 351 (0 to 1,695). The mean LM-LAD arteries angle was 40.3° ± 10.0° in 39 patients whose CACS was ≥200, versus 33.8° ± 11.6° in 82 patients with CACS <200 (<i>p</i> = 0.003). A weak, but positive correlation (r = 0.269, <i>p</i> = 0.003) was observed between the LM-LAD arteries angle and CACS of the LM-LAD arteries. By multiple variable analysis, hemoglobin A1c, triglycerides, eGFR and the LM-LAD arteries angle were independent predictors of a high CACS of the LM-LAD arteries.</p><p>Conclusion</p><p>In patients with CKD, a wide LM-LAD arteries angle was associated with a high CACS of the LM-LAD arteries. The prognostic value of this observation warrants further evaluation.</p></div

    Behavioral and Proteomic Analysis of Stress Response in Zebrafish (<i>Danio rerio</i>)

    No full text
    The purpose of this study is to determine the behavioral and proteomic consequences of shock-induced stress in zebrafish (<i>Danio rerio</i>) as a vertebrate model. Here we describe the behavioral effects of exposure to predictable and unpredictable electric shock, together with quantitative tandem mass tag isobaric labeling workflow to detect altered protein candidates in response to shock exposure. Behavioral results demonstrate a hyperactivity response to electric shock and a suppression of activity to a stimulus predicting shock. On the basis of the quantitative changes in protein abundance following shock exposure, eight proteins were significantly up-regulated (HADHB, hspa8, hspa5, actb1, mych4, atp2a1, zgc:86709, and zgc:86725). These proteins contribute crucially in catalytic activities, stress response, cation transport, and motor activities. This behavioral proteomic driven study clearly showed that besides the rapid induction of heat shock proteins, other catalytic enzymes and cation transporters were rapidly elevated as a mechanism to counteract oxidative stress conditions resulting from elevated fear/anxiety levels

    Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS

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    Large-scale proteomics often employs two orthogonal separation methods to fractionate complex peptide mixtures. Fractionation can involve ion exchange separation coupled to reversed-phase separation or, more recently, two reversed-phase separations performed at different pH values. When multidimensional separations are combined with tandem mass spectrometry for protein identification, the strategy is often referred to as multidimensional protein identification technology (MudPIT). MudPIT has been used in either an automated (online) or manual (offline) format. In this study, we evaluated the performance of different MudPIT strategies by both label-free and tandem mass tag (TMT) isobaric tagging. Our findings revealed that online MudPIT provided more peptide/protein identifications and higher sequence coverage than offline platforms. When employing an off-line fractionation method with direct loading of samples onto the column from an eppendorf tube via a high-pressure device, a 5.3% loss in protein identifications is observed. When off-line fractionated samples are loaded via an autosampler, a 44.5% loss in protein identifications is observed compared with direct loading of samples onto a triphasic capillary column. Moreover, peptide recovery was significantly lower after offline fractionation than in online fractionation. Signal-to-noise (S/N) ratio, however, was not significantly altered between experimental groups. It is likely that offline sample collection results in stochastic peptide loss due to noncovalent adsorption to solid surfaces. Therefore, the use of the offline approaches should be considered carefully when processing minute quantities of valuable samples

    A weak positive correlation between the LM-LAD arteries angle and the CACS of the LM-LAD arteries (r = 0.269; <i>p</i> = 0.003).

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    <p>A weak positive correlation between the LM-LAD arteries angle and the CACS of the LM-LAD arteries (r = 0.269; <i>p</i> = 0.003).</p

    Baseline characteristics of 82 patients with <200 versus 39 patients with ≥200 coronary artery calcium score (CACS) of the left main (LM)-left anterior descending (LAD) arteries.

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    <p>Baseline characteristics of 82 patients with <200 versus 39 patients with ≥200 coronary artery calcium score (CACS) of the left main (LM)-left anterior descending (LAD) arteries.</p

    Comparison of diagnostic characteristics in model 1 (hemoglobin A1c, triglyceride, eGFR, LM-LAD arteries angle) versus model 2 (hemoglobin A1c, triglyceride, eGFR) to predict severe calcifications of the LM-LAD arteries.

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    <p>The accuracy of the C statistics in the prediction of a high CACS in the LM-LAD arteries was increased by adding the measurement of the LM-LAD arteries angle (area under the curve = 0.816 in model 1 versus 0.769 in model 2).</p

    Outcomes of single and multiple variable logistic regression analyses of correlates of CACS of the LM-LAD arteries <200 versus ≥200.

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    <p>Outcomes of single and multiple variable logistic regression analyses of correlates of CACS of the LM-LAD arteries <200 versus ≥200.</p

    Classification of all protein-coding genes based on their expression in human urinary bladder tissue.

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    <p>(A) Scatterplot showing the FPKM values of all 20,344 protein-coding genes in the two urinary bladder samples, with each gene colored according to category. (B) Pie chart showing the distribution of all protein-coding genes into five categories based on transcript abundance and number of detected tissues, including expression in all 32 tissues (blue), mixed expression with genes expressed in a varying number of tissue (green), genes with elevated expression in urinary bladder (pink), not detected in urinary bladder (light grey), and not detected in any tissue (dark grey). The genes with elevated expression in urinary bladder are further subdivided depending on the degree of specificity as tissue enriched genes, group enriched genes and tissue enhanced genes in urinary bladder (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145301#pone.0145301.t001" target="_blank">Table 1</a>). (C) Pie chart showing the distribution of the fraction of expressed mRNA molecules, i.e. the sum of all FPKM values for each of the categories for the genes expressed in urinary bladder, using the same color codes. (D) Network plot of the urinary bladder enriched gene (red) and group enriched genes (orange). Orange circle nodes represent a shared group of expressed genes and are connected to the respective enriched tissues (grey circles). The size of each orange node is related to the square root of the number of genes enriched in a particular combination of tissues.</p
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