2 research outputs found

    Exploring S-Nitrosoglutathione reductase function in the non-vascular plant, Marchantia polymorpha

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    A key feature of plant immunity is the rapid engagement of a burst of nitric oxide (NO) following pathogen recognition. In addition to direct antimicrobial activity NO also orchestrates a plethora of signalling functions to control the deployment of plant immune responses. The predominant route for NO signalling is S-nitrosylation, a key redox-based, post-translational modification involving the addition of a NO moiety to a protein cysteine (Cys) thiol, forming an S-nitrosothiol (SNO). To explore a potential role for S-nitrosylation in the immune system of early land plants, we identified a single-copy S-nitrosoglutathione reductase 1 (GSNOR1) gene in Marchantia polymorpha (Mp) and introduced loss-of-function mutations using the CRISPR/Cas9 gene editing method to explore the potential function of GSNOR in this early land plant. We observed distinct morphological changes in Mp resulting from the absence of GSNOR1 function, providing new insights into the structural adaptations of this plant. In addition, we found GSNOR1 plays a central role in the immune system of Mp. This research contributes to a broader understanding of plant-microbe interactions. It offers a fundamental understanding of the function of NO and GSNOR1 in Mp development and immunity and also provides insights into evolutionary plant biology

    Data from: An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor

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    Plants use autophagy to safeguard against infectious diseases. However, how plant pathogens interfere with autophagy related processes is unknown. Here we show that PexRD54, an effector from the Irish potato famine pathogen Phytophthora infestans, binds host autophagy protein ATG8CL to stimulate autophagosome formation. PexRD54 depletes the autophagy cargo receptor Joka2 out of ATG8CL complexes and interferes with Joka2's positive effect on pathogen defense. Thus a plant pathogen effector has evolved to antagonize a host autophagy cargo receptor in order to counteract host defenses